Lcq deca ion trap

Manufactured by Thermo Fisher Scientific

The LCQ Deca Ion-Trap is a mass spectrometry instrument designed for sensitive and accurate analysis of a wide range of molecules. It utilizes an ion-trap technology to capture, isolate, and detect ions, providing high-quality data for various applications.

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Lab products found in correlation

Ltq orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

LCQ-DECA ion trap mass spectrometer LCQ Deca XP plus ion trap LCQ Deca XP ion-trap mass spectrometer Finnigan LCQ DECA ion trap LCQ Deca XPplus ion trap mass spectrometer LCQ Deca

3 protocols using lcq deca ion trap

MALDI-MS Analysis of Folates in Bacteria

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Cell pellets normalized by weight from wild‐type and mutant L. reuteri strains were washed with ice‐cold PBS and water. Folates were extracted by washing with 50% acetonitrile/0.1% v/v TFA. The cell suspension was centrifuged for 10 min, 4000g at 4°C. Samples were prepared with α‐cyano‐4‐hydroxy‐cinnamic acid as MALDI matrix, spotted onto a sample plate, dried, and analyzed on a prOTOF2000 MALDI mass spectrometer (PerkinElmer/Sciex, Boston, MA) or on a SimulTOF Combo 200 MALDI mass spectrometer (Virgin Instruments Marlborough, MA). For MS/MS fragmentation analysis, the same MALDI sample plate was subsequently transferred into a self‐built MALDI quadrupole ion trap that was based on a modified LCQ DECA iontrap (Thermo Waltham, MA) (Krutchinsky et al. 2001). Tetrahydrofolate ions were fragmented with a 4 Da selection window, 30% collision energy, a fixed ion trap injection time of 200 msec, and a 2–10‐hz laser repetition rate.

Thomas C.M., Saulnier D.M., Spinler J.K., Hemarajata P., Gao C., Jones S.E., Grimm A., Balderas M.A., Burstein M.D., Morra C., Roeth D., Kalkum M, & Versalovic J. (2016). FolC2‐mediated folate metabolism contributes to suppression of inflammation by probiotic Lactobacillus reuteri. MicrobiologyOpen, 5(5), 802-818.

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Elucidation of Positive-Ion Precursors

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Formation of the positive-ion precursors, via protonation, was achieved by ESI from 1:1 acetonitrile/water by direct infusion (5 μL/min). Low resolving-power MS and CA (MS/MS and MS³) were with a Thermo LCQ Deca Ion-Trap (San Jose, CA) as survey experiments. Analogous high resolving-power experiments were performed with a Thermo LTQ-Orbitrap mass spectrometer operated in the positive-ion mode (RP for product ions was 30000 for both MS/MS and MS³). To track fragmentation, acidic H atoms were replaced with D, in a 1:1 D₂O/acetonitrile mixture and introduced by direct infusion, ionized by ESI, and analyzed.

Cyriac J., Paulose J., George M., Srinivas R., Giblin D, & Gross M.L. (2019). Protonation of Curcumin Triggers Sequential Double Cyclization in the Gas-Phase: An Electrospray Mass Spectrometry and DFT Study. International journal of mass spectrometry, 438, 107-114.

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Positive-ion Mode Mass Spectrometry Analysis

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ESI mass spectra of the compounds were obtained by direct infusion (5
μL/min) using a dilute solution of the amines in a 1:1 mixture of
acetonitrile/water. The low mass resolving-power MS and CAD (MS/MS) experiments
using helium as collision gas were performed with a Thermo LCQ Deca Ion-Trap
(San Jose, CA) mass spectrometer operated in the positive-ion mode. Dry nitrogen
gas at 90 ⁰C was used for desolvation. High mass resolving-power CAD
(collision gas – helium) experiments were performed with a Thermo
LTQ-Orbitrap mass spectrometer operated in the positive-ion mode (resolving
power for product ions was 30,000 for MS/MS). The collision energy was adjusted
to keep the abundances of the precursor ion and the most abundant product ion to
be similar.

Paulose J., Cyriac J., Mathai G., Giblin D, & Gross M.L. (2016). Protonated N-Alkyl-2-nitroanilines Undergo Intramolecular Oxidation of the Alkyl Chain upon Collisional Activation. International journal of mass spectrometry, 413, 75-80.

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