Fatp2

Liver tissue samples and HepG2 cells were lysed with RIPA lysis buffer (Apply Gene, Beijing, China) containing 1% PMSF. The primary antibodies against CD36 (1:1,000, Abcam, Cambridge, United Kingdom), GAPDH (1:5,000, Proteintech, Chicago, United States), FATP2 (1:1,000, Proteintech, Chicago, United States), and FATP5 (Bioss, Beijing, China) were used. After incubating overnight at 4°C, the corresponding HRP-conjugated anti-rabbit IgG secondary antibodies (1:5,000, Proteintech, Chicago, IL, United States) were used.

Naringin and D (+)-glucose were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Rutin was from Shi Feng Biotechnology Co., Ltd. (Shanghai, China). Total cholesterol (TC), triglyceride (TG), and hematoxylin-eosin (H&E) reagent were all purchased from Nanjing Jiancheng Technology Co., Ltd. (Jiangsu, China). CPT1-α, FATP2, SCD-1, ACOX-1, FAS, HRP conjugated goat anti-rabbit IgG (H + L), and GAPDH antibodies were all purchased from Proteintech Group (Chicago, USA). HRP conjugated goat anti-mouse/rabbit IgG (PV-6001) was from Zhongshan Goldenbridge Biotechnology Co., Ltd. (Beijing, China). BCA protein concentration determination kit, RIPA lysate kit, and chemiluminescent assay kit were from Beyotime Biotechnology. (Shanghai, China). All triheptadecenoyl glycerol (T17 : 1 TAG) was purchased from Nu Chek, Inc. (Elysian, USA). All other reagents were at least analytical grade and purchased from Fisher Scientific (Pittsburgh, USA), Sigma-Aldrich Chemical Company (St. Louis, USA), or as specified.