Salidroside (Macklin, S817419, purity > 98%), Dimethyl sulfoxide (DMSO, sigma-Aldrich, D2650), Ferrostatin-1(Fer-1, Topscience, 347174-05-4) was dissolved in DMSO and freshly diluted to the final concentration (0.05% DMSO) in the study, Glutamate(Glu, sigma-Aldrich, G1626), Amyloid β Protein Fragment 1–42(Aβ1− 42, sigma-Aldrich, G1626), Cell Counting Kit-8 assay kit (CCK-8, bimake, B34304, USA), Lactate Dehydrogenase (LDH) detection kit (Best Bio, China, BB-4860-500T), Reactive Oxygen Species (ROS) assay kit (Nanjing jiancheng, E004-1-1), JC-1(Solarbio, J8030), C11 BODIPY 581/591(GLPBIO, 217075-36-0), FerroOrange Cell Ferrous Ion Fluorescence Probe (Dojindo, Japan, F374), Lipid Peroxidation MDA Assay Kit (Beyotime, S0131S), Total Superoxide Dismutase Assay Kit with WST-8 (Beyotime, S0101S), GSH and GSSG Assay Kit (Beyotime, S0053), LipoInsect™ Transfection Reagent (Beyotime, C0526-1.5mL), Opti-MEM™ I Reduced Serum Medium (Gibco, 31985070), Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H + L) (Beyotime, A0423), GPX4 Antibody (Affinity, DF6701), SLC7A11 Polyclonal Antibody (Proteintech, 26864-1-AP), HO1 Antibody (Affinity, AF5393), Nrf2 Polyclonal Antibody (Proteintech, 16396-1-AP), PCNA Polyclonal Antibody (Proteintech, 10205-2-AP), β-Actin (D6A8) Rabbit mAb (Cell Signaling Technology, #8457), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (Proteintech, SA00001-2).
Pcna polyclonal antibody
Manufactured by Proteintech
Sourced in Japan, China
The PCNA Polyclonal Antibody is a laboratory reagent used to detect the Proliferating Cell Nuclear Antigen (PCNA) protein. PCNA is a protein involved in cell cycle regulation and DNA replication.
Automatically generated - may contain errors
Lab products found in correlation
Glutamate glu, by Merck Group (1 mentions)
Salidroside, by Merck Group (1 mentions)
Ferrostatin 1, by Topscience (1 mentions)
Amyloid β protein fragment 1 42, by Merck Group (1 mentions)
Cell counting kit 8 assay kit, by Selleck Chemicals (1 mentions)
Lipoinsect transfection reagent, by Beyotime (1 mentions)
Nrf2 polyclonal antibody, by Proteintech (1 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
β actin, by Proteintech (1 mentions)
Total superoxide dismutase assay kit with wst 8, by Beyotime (1 mentions)
Gsh and gssg assay kit, by Beyotime (1 mentions)
Lipid peroxidation mda assay kit, by Beyotime (1 mentions)
Alexa fluor 488 labeled goat anti rabbit igg h l, by Beyotime (1 mentions)
Reactive oxygen species assay kit, by Nanjing Jiancheng (1 mentions)
Diaminobenzidine dab, by Beijing Strong Biotechnologies (1 mentions)
Biotin labeled goat anti rabbit igg, by Abcam (1 mentions)
Goat anti rabbit hrp conjugate, by Cell Signaling Technology (1 mentions)
Anti e cadherin antibody, by Abcam (1 mentions)
Vimentin polyclonal antibody, by Proteintech (1 mentions)
4 protocols using pcna polyclonal antibody
1
Salidroside Mitigates Amyloid-Beta Toxicity
2
Immunohistochemical Analysis of Proliferation and Smooth Muscle
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Paraffin-embedded sections of the CCA were cut for each group of rats, routinely dewaxed, hydrated, and heated in citrate antigen retrieval solution for antigen retrieval. The sections were blocked with 5% bovine serum albumin (BSA) and then incubated with an antiproliferating cell nuclear antigen (PCNA) polyclonal antibody (1 : 200; Proteintech, China) and anti-SMα-actin polyclonal antibody (1 : 200; CST, USA) overnight at 4°C followed by biotin-labeled goat anti-rabbit IgG (1 : 2000; Abcam, USA) at 37°C in the dark for 30 min. Then, diaminobenzidine (DAB; MXB, China) was used for color development, images were captured and saved with the ISCapture system, and Image-Pro Plus 6 image processing software was used for data analysis.
3
Immunohistochemical Analysis of Cancer Markers
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The paraffin tissue sections were subjected to dewaxing and rehydration procedures. After performing antigen retrieval, the slides were subjected to blocking using bovine serum albumin (Sango Biotech, Shanghai, China). Subsequently, the slides were incubated overnight at 4 °C with c-Fos Monoclonal antibody (Proteintech, 66590-1-Ig), Vimentin Polyclonal antibody (Proteintech, 10366-1-AP), Anti-E-cadherin antibody (Abcam, ab231303), PCNA Polyclonal antibody (Proteintech, 10205-2-AP), and HIF-1 alpha Polyclonal antibody (Proteintech, 20960-1-AP). Subsequently, the samples were incubated with a secondary antibody of goat anti-rabbit HRP conjugate (Cell Signaling Technology, Beverly, MA, USA) at 25 °C for 1 h. A DAB solution was employed to facilitate brown color development. The regions of interests (ROIs) were analyzed using IHC Profiler [20 (link)].
4
Immunohistochemical Analysis of Tumor Tissues
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Paraffin-embedded tumor tissues from patients and nude mice were sectioned to generate 5 μm sections, which were deparaffinized and rehydrated. After blocking endogenous avidin and biotin, antigen retrieval was performed using sodium citrate buffer at high temperature for 20 min. The antigens were blocked with BSA for 30 min, and sections were incubated with antibodies overnight at 4 °C. The antibodies used were as follows: RACGAP1 polyclonal antibody (Proteintech, cat no. 13739-1-AP, dilution 1:200), MCM3 polyclonal antibody (Proteintech, cat. no. 15597-1-AP, dilution 1:200), PCNA polyclonal antibody (Proteintech, cat. no. 10205-2-AP, dilution 1:100) and MKI67 (Proteintech, cat. no. 27309-1-AP, dilution 1:200). On the following day, the sections were incubated with secondary antibodies, washed three times, stained with DAB for 5 min, counterstained with hematoxylin for 5 min, dehydrated, and sealed with neutral resin. Images were acquired with a digital scanner, and the results were analyzed by ImageJ software.
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