Fixed and saponin permeabilized SKM-GLUT4 cells where stained with either 1:500 anti-ATP7A (Lifespan Biosciences, LS-C209614) or 1:500 anti-TGN38 (Abcam ab16059) antibodies followed by 1:300 Cy3 goat anti-mouse IgG (115-165-062 Jackson Research).
Ab16059
Manufactured by Abcam
Ab16059 is a lab equipment product manufactured by Abcam. It is a highly specific and sensitive antibody designed for the detection of target proteins in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab24170, by Abcam (2 mentions)
Biotinylated anti mouse antibody, by Vector Laboratories (1 mentions)
Ab18211, by Abcam (1 mentions)
Ab21685, by Abcam (1 mentions)
Ab22595, by Abcam (1 mentions)
Ab21679, by Abcam (1 mentions)
Ab46540, by Abcam (1 mentions)
Ab52649, by Abcam (1 mentions)
Prb 435p, by Fortrea (1 mentions)
Na934v, by Cytiva (1 mentions)
Na931v, by Cytiva (1 mentions)
Ab12223, by Abcam (1 mentions)
Ab13523, by Abcam (1 mentions)
Ab8245, by Abcam (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Merck Group (1 mentions)
Ab137029, by Abcam (1 mentions)
Lamp1, by Abcam (1 mentions)
Ci m6pr, by Abcam (1 mentions)
A11008, by Thermo Fisher Scientific (1 mentions)
5 protocols using ab16059
1
Visualizing ATP7A and TGN38 in SKM-GLUT4 Cells
2
Comprehensive Immunofluorescence Labeling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following primary antibodies were used in the present study: mouse anti-Ttyh1 (1:200, #WH0057348M4, Sigma), rabbit anti-Synaptotagmin 1 (1:100, #105102, Synaptic Systems), rabbit anti-Synaptoporin (1:500, #102002, Synaptic Systems), rabbit anti-Neuronal Class III β-Tubulin (1:750, #PRB-435P, Covance), rabbit anti-LAMP1 (1:500, #ab24170, Abcam), rabbit anti-GRP78 (1:200, #ab21685, Abcam), rabbit anti-Calnexin (1:40, #ab22595, Abcam), rabbit anti-GM130 (1:200, #ab52649, Abcam), rabbit anti-TGN46 (1:500, #ab16059), rabbit anti-Clathrin (1:500, #ab21679, Abcam), rabbit anti-Rab5 (1:500, #ab18211, Abcam), rabbit anti-Rab11 (1:100, #71-5300, Invitrogen), rabbit anti-APPL2 (home made, [20 (link)]), rabbit anti-Olig2 (1:500, #AB9610, Chemicon Millipore), rabbit anti-Iba1 (1:1,000, #019-19741, Wako), mouse anti-GFAP-Cy3 (1:1,000, #C9205, Sigma). Anti-IgG mouse (1:200, #553447, BD Pharmingen) anti-IgG rabbit (1:200, #ab46540, Abcam) were used as controls to test specificity of immunostainings. The following secondary antibodies were used in the present study: goat anti-mouse conjugated with Alexa Fluor 488 (1:200, #A11001, Molecular Probes), goat anti-rabbit conjugated with Texas Red (1:200, #T2767, Molecular Probes), horse anti-mouse conjugated with Texas Red (1:200, #TI2000, Vector Laboratories), and anti-mouse biotinylated antibody (1:200, #BA2001, Vector Laboratories).
3
Generation and Characterization of Antibodies for RCMV Proteins
4
Antibodies for Western Blot and Immunocytochemistry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used for western blot: actin (Sigma, A3853, Ms, 1:1000), CatD (R&D Systems, AF1029, Gt, 1:1000), CD63 (Abcam, EPR21151-ab217345, Rb, 1:1000), ci-mannose 6-phosphate receptor (M6PR) (Cell Signaling Technology, 14364S, Rb, 1:500), GAPDH (Sigma, G8795, Ms, 1:40 000), LAMP1 (DSHB, 1D4B, Rt, 1:1000), RAB5 (Cell Signaling Technology, 3547, Rb, 1:1000), and RAB7 (Sigma, R8779, Ms, 1:1000). The following antibodies were used for immunocytochemistry experiments: ci-M6PR (Abcam, 2G11-ab2733, Ms, 1:1000), Hoechst-33342 (Thermo Fisher Scientific, H1399, 1:1600), LAMP1 (Abcam, ab24170, Rb, 1:500 for BODIPY-pepstatin A, 1:1000 for M6PR), lysobisphosphatidic acid (LBPA, Echelon Biosciences, Ms, 1:100), MAP2 (Abcam, ab5392, Ch, 1:5000), RAB5 (Cell Signaling Technology, 3547, Rb, 1:500), RAB7 (Abcam, ab137029, Rb, 1:100 for BODIPY-pepstatin A, 1:500 for M6PR and 3D imaging), and TGN46 (Abcam, ab16059, Rb, 1:1000). The following secondary antibodies were used at a 1:800 dilution: anti-mouse AlexaFluor-488, anti-chicken AlexaFluor-594, anti-rabbit AlexaFluor-594, anti-mouse AlexaFluor-647, anti-rabbit AlexaFluor-647, and anti-rat AlexaFluor-647.
5
Immunostaining of Proteins and Lipids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunostaining of proteins and lipids was used in fixed cells with 4% (wt/vol) paraformaldehyde. For surface proteins, nonpermeabilized cells were directly blocked with goat serum and then incubated with the appropriate primary antibody. For internal proteins and lipids, cells were permeabilized with 0.1% (vol/vol) Triton X-100 before blocking and incubation with the primary antibody. Control experiments were performed to make sure that chemical fixation did not induce permeabilization (see Fig. S1 B ). For surface GluA1 analysis, isolated primary dendrites close to the soma of the neuron were selected. Rabbit anti-GluA1 (1:30; PC246) was from Calbiochem. Mouse anti-PI(4)P (1:100; Z-P004) was from Echelon. Mouse anti-TGN38 (1:100; NB300-575) was from Novus Biologicals. Rabbit anti-TGN46 (1:100; ab16059) and chicken anti-GFP (1:1,000; ab13970) were from Abcam. Rabbit anti-SAC1 (1:100; 13033–1-AP) was from Proteintech. Incubation with the appropriate Alexa Fluor–conjugated secondary antibodies was also performed. Anti-mouse Alexa633 (1:100; A21052), anti-rabbit Alexa488 (1:300; A11008), anti-chicken Alexa488 (1:1,000; A11039), and anti-mouse Alexa568 (1:150; A11011) were from Invitrogen. Coverslips were mounted with Fluoromount Aqueous Mounting Medium (F4680; Sigma-Aldrich).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!