Pd l1 mih1

Manufactured by BD

Sourced in United States

The PD-L1 (MIH1) is a laboratory equipment product designed to detect and analyze the expression of the PD-L1 protein. It serves as a tool for researchers and scientists to study immune checkpoint pathways and their role in various biological and disease processes.

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Lab products found in correlation

Lsrfortessa, by BD (3 mentions) Cd80 l307.4, by BD (2 mentions) Cd25 2a3, by BD (2 mentions) Cd209 dcn46, by BD (2 mentions) Pd 1 eh12.2h7, by BioLegend (1 mentions) Tigit mbsa43, by Thermo Fisher Scientific (1 mentions) Facscanto 2 flow cytometer, by BioLegend (1 mentions) Nkg2d bat221, by Miltenyi Biotec (1 mentions) Nkg2a rea110, by Miltenyi Biotec (1 mentions) Live dead dye, by Thermo Fisher Scientific (1 mentions) Cd45 hi30, by BioLegend (1 mentions) Hla dr l243, by Thermo Fisher Scientific (1 mentions) Version 9, by FlowJo (1 mentions) 7 aminoactinomycin d 7aad live dead dye, by Thermo Fisher Scientific (1 mentions) Cd206, by BioLegend (1 mentions) Fc block, by BD (1 mentions) Live dead aqua staining, by Thermo Fisher Scientific (1 mentions) Version 10, by FlowJo (1 mentions)

Spelling variants of this product

PD-L1 (44 citations) PD-L1 (clone MIH1, (4 citations) Anti-PD-L1 (MIH1, (3 citations) PD-L1-BV421 (clone MIH1, (2 citations)

4 protocols using pd l1 mih1

Characterization of Engineered NK-92 Cells

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NK-92, NK-92/hu14.18.28.z, and NK-92/hu14.18.28.z_RD-IL15 cells were incubated at 37 °C with K562 and EL4 target cells at an initial E/T ratio of 1:2, with fresh target cells again added to the effector cells after 24, 48, and 72 h. One day later, surface expression of marker proteins was analyzed by flow cytometry with fluorochrome-conjugated antibodies specific for NKp30 (p30-15; BD Biosciences), NKp44 (p44-8; BD Biosciences), NKp46 (9E2; Miltenyi Biotec, Bergisch Gladbach, Germany), NKG2D (BAT221; Miltenyi Biotec), NKG2A (REA110; Miltenyi Biotec), KIR2D (NKVFS1; Miltenyi Biotec), CD96 (NK92.39; Invitrogen), PD-1 (EH12.2H7; BioLegend), PD-L1 (MIH1; BD Biosciences), LAG-3 (7H2C65; BioLegend), TIGIT (MBSA43; Invitrogen), and TIM-3 (F38-2E2; BioLegend) using a FACSCanto II flow cytometer. Parental and CAR-engineered NK-92 cells cultured without target cells served as controls. Data were analyzed using FACSDiva software.

Bodden M., Häcker A., Röder J., Kiefer A., Zhang C., Bhatti A., Pfeifer Serrahima J., Ullrich E., Kühnel I, & Wels W.S. (2023). Co-Expression of an IL-15 Superagonist Facilitates Self-Enrichment of GD2-Targeted CAR-NK Cells and Mediates Potent Cell Killing in the Absence of IL-2. Cancers, 15(17), 4310.

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Characterization of Dermal APCs upon Plasmodium Stimulation

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Lysed single cell skin suspensions were counted and plated out in 48 well plates at 2*10⁵ cells per well. Cells were stimulated with mCherry expressing Pb or Wild-type Pf SPZ for 1 hour (Pb uptake experiments) or 24 hours (Pb and Pf stimulated dermal APC phenotyping). Cells were stained with 7AAD live/dead dye (uptake) or with Aqua fixable live/dead dye (Thermo Fischer Scientific; phenotyping), CD45 (HI30; Biolegend), HLA-DR (L243; eBioscience), CD11c (Bu15, Biolegend), PD-L1 (MIH1; BD Biosiences), CD80 (L307.4; BD Biosciences) and analyzed by Flow Cytometry using an LSRFortessa (BD Biosciences). Data was analyzed in FlowJo version 9.9.6 (FlowJo LLC). Gates were set using ‘fluorescence minus one’ (FMO) stained control samples.

Winkel B.M., Pelgrom L.R., van Schuijlenburg R., Baalbergen E., Ganesh M.S., Gerritsma H., de Korne C.M., Duszenko N., Langenberg M.C., Chevalley-Maurel S.C., Smits H.H., de Jong E.C., Everts B., Franke-Fayard B, & Roestenberg M. (2020). Plasmodium sporozoites induce regulatory macrophages. PLoS Pathogens, 16(9), e1008799.

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Immunophenotyping of Pf Sporozoite-Exposed MoDCs and MoMϕ

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On day 7, rested immature MoDCs and M0 MoMϕ were exposed for 24 hours to recombinant pf circumsporozoite protein (recCSP; Alpha Diagnostics, San Antonio, TX, USA catalognr: CSPF17-R-10), 20.000 Plasmodium falciparum (Pf) SPZ, a matched volume of salivary gland extract (SGE) of uninfected control mosquitoes or medium as a true negative control and ultrapure lipopolysaccharide (LPS; 100ng/ml; Escherichia coli 0111 B4 strain, InvivoGen, San Diego, CA, USA) as an inflammatory control. When different well sizes were used, the same 1:5 sporozoite:cell ratio was maintained. Importantly, SGE was used as a matched control as SPZ preparations do contain traces of salivary gland material. After 24 hour, cells were harvested, stained with 7-aminoactinomycin D (7AAD) live/dead dye (Invitrogen, Waltham, MA, USA), and antibodies against CD80 (L307.4; BD Biosciences), CD86 (N331 FUN-1; BD Biosciences), CD25 (2A3; BD Biosciences), CD197 (3D12; BD Biosciences), CD206 (15–2; Biolegend), CD209 (DCN46; BD Biosciences), PD-L1 (MIH1; BD Biosciences), PD-L2 (MIH18; eBiosience) and Immunoglobulin-like transcript 3 (ILT3; zm4.1; Biolegend) and analyzed by Flow Cytometry using a BD LSR Fortessa (BD Biosciences, San Jose, CA, USA) and analyzed in FlowJo version 9.9.6 (FlowJo LLC, Ashland, OR, USA).

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Phenotypic Characterization of Monocyte-Derived Macrophages

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In addition, the phenotype of MoMɸs was assessed by flow cytometry and cytokine measurements. For this purpose, MoMɸs were stimulated with 1:1 ratio SPZ, salivary gland extract (SGE) as a negative control, medium as background or 100 ng/mL lipopolysaccharide (LPS) as a positive control for 24 h at 37 °C + 5% CO₂. After 24 h supernatant was collected and the cells were harvested, washed with PBS and stained for CD209 (DCN46; BD Bioscience), CD163 (9332, BD Bioscience), PD-L1 (MIH1; BD Bioscience) ILT3 (zm4.1; Biolegend), CD206 (15-2; Biolegend), CD80 (L307.3; BD Bioscience), CD25 (2A3; BD Bioscience), aqua live/dead staining (Thermofisher), Fc-block (BD Bioscience) in PBS for 30 min at 4 °C and measured by flow cytometry using LSR Fortessa (BD bioscience, San Jose, CA, USA). The data was analysed by FlowJo version 10.8 (FlowJo LLC, Ashland, OR, USA). After 24 h stimulation of MoMɸs with SPZ from day 14, 17 and 20 post blood meal, and controls (LPS, SGE, medium) the supernatants were diluted 1:5 (IL6) or 1:2 (IL10 and TNF) and analysed by standard ELISA (Sanquin, The Netherlands).

van Schuijlenburg R., Azargoshasb S., de Korne C.M., Sijtsma J.C., Bezemer S., van der Ham A.J., Baalbergen E., Geurten F., de Bes-Roeleveld L.M., Chevalley-Maurel S.C., van Oosterom M.N., van Leeuwen F.W., Franke-Fayard B, & Roestenberg M. (2024). Ageing of Plasmodium falciparum malaria sporozoites alters their motility, infectivity and reduces immune activation in vitro. Malaria Journal, 23, 111.

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