Ia ie bv421

Manufactured by BioLegend

Sourced in United States

The IA/IE-BV421 is a fluorescently labeled antibody product from BioLegend. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd11c percp cy5, by BioLegend (2 mentions) Cd45 bv510 30 f11, by BioLegend (2 mentions) Ly6c pe hk1.4, by BioLegend (2 mentions) Cd8 percpcy5.5 53 6 7, by BioLegend (2 mentions) Gr1 apc cy7 rb6 8c5, by BioLegend (2 mentions) Cd11b pe cy7, by BioLegend (2 mentions) Facscanto 2, by BD (2 mentions) Fc block, by BD (2 mentions) F4 80 fitc bm8, by BioLegend (1 mentions) Il 10 pe, by BD (1 mentions) Cd25 pe cy7, by BioLegend (1 mentions) Ifnγ bv421, by BioLegend (1 mentions) B220 ra3 6b2, by BD (1 mentions) Cd25 pe cy7 pc61, by BioLegend (1 mentions) B220 apc cy7 ra3 6b2, by BD (1 mentions) Cd3 apc 17a2, by BioLegend (1 mentions) Cd4 bv421 rm4 5, by BioLegend (1 mentions) F4 80 fitc, by BioLegend (1 mentions) Epcam apc cy7, by BioLegend (1 mentions) Cd8 bv711, by BD (1 mentions)

3 protocols using ia ie bv421

Multiparametric Flow Cytometry of Immune Cells

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Cells were incubated with Fc Block (1 µg/10⁶ cells; 2.4G2; BD Biosciences) for 15 min. Extracellular staining was performed for 30 min on ice using: CD4‐BV521 (RM4‐5; BioLegend, San Diego, CA, USA), CD45‐BV510 (30‐F11; BioLegend), CD3‐APC (17.A2, BioLegend), CD25‐PE‐Cy7 (PC61; BioLegend), CD8‐PerCPCy5.5 (53‐6.7; BioLegend), B220 (RA3‐6B2; BD Biosciences), CD11b‐PE‐Cy7 (M1/70; BioLegend), Ly6C‐PE (HK1.4; BioLegend), Gr1‐APC‐Cy7 (RB6‐8C5, BioLegend), IA/IE‐BV421 (M5/114.15.2, BioLegend), F4/80‐FITC (BM8, BioLegend), CD11c‐PerCPCy5.5 (N418, BioLegend).
After staining for extracellular proteins, cells were fixed in 4% PFA and permeabilised using 0.1% saponin buffer containing 0.1% bovine serum albumin. Intracellular cytokines were detected with IFNγ‐BV421 (XMG 1.2, BioLegend), IL‐10‐PE (54902, BD Bioscience), and IL‐17A‐AF647 (TC11‐18H10, BioLegend).
Flow cytometry was performed on a BD FACS Canto II (BD Biosciences) and analysed using FlowJo software version 10.6 (Treestar Inc., Ashland, OR, USA).

Denny L., Al Abadey A., Robichon K., Templeton N., Prisinzano T.E., Kivell B.M, & La Flamme A.C. (2021). Nalfurafine reduces neuroinflammation and drives remyelination in models of CNS demyelinating disease. Clinical & Translational Immunology, 10(1), e1234.

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Multiparametric Analysis of Immune Cells

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Cells were incubated with Fc Block (1 μg/10⁶ cells; 2.4G2; BD Biosciences) for 15 min. Extracellular staining was performed for 30 min on ice using the following antibodies: CD4-BV421 (RM4-5; BioLegend, San Diego, CA, USA), CD45-BV510 (30-F11; BioLegend), CD3-APC (17.A2, BioLegend), CD25-PE-Cy7 (PC61; BioLegend), CD8 PerCPCy5.5 (53-6.7; BioLegend), B220-APC-Cy7 (RA3-6B2; BD Biosciences), CD11b-PE-Cy7 (M1/70; BioLegend), Ly6C-PE (HK1.4; BioLegend), Gr1-APC-Cy7 (RB6-8C5; BioLegend), IA/IE-BV421 (M5/114.15.2; BioLegend), F4/80-FITC (BM8; BioLegend), CD11c-PerCP-Cy5.5 (N418; BioLegend).
After staining for extracellular proteins, cells were fixed in 4% paraformaldehyde (PFA, pH 7.4) and permeabilized using 0.1% saponin buffer containing 0.1% bovine serum albumin. For intracellular cytokine detection, interferon-γ (IFNγ)-BV421 (XMG 1.2, BioLegend), interleukin (IL)-10-PE (54902, BD Bioscience), and IL-17A-AF647 (TC11-18H10, BioLegend) antibodies were used.
Flow cytometry was performed on a BD FACS Canto II (BD Biosciences) and analyzed using FlowJo software version 10.1 (Treestar Inc., Ashland, OR, USA).

Paton K.F., Robichon K., Templeton N., Denny L., Al Abadey A., Luo D., Prisinzano T.E., La Flamme A.C, & Kivell B.M. (2021). The Salvinorin Analogue, Ethoxymethyl Ether Salvinorin B, Promotes Remyelination in Preclinical Models of Multiple Sclerosis. Frontiers in Neurology, 12, 782190.

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Multiparameter Flow Cytometry of Immune Cells

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Single cell suspensions from mouse experiments were stained with Live/dead Aqua (Molecular Probes, Inc.), CD4-PerCP, CD8-Pacific Blue, CD69-BV605, CD62E-PE, CD62E-BV421, CD62P-Alexa647, CD162-AlexaFlour647, HECA454-PE (BD Bioscience), CD25-APC, CD31-BV605, CD105-Pacblue, I/A-I/E-BV421, CD45-APCCy7, EpCAM-APC-Cy7, Podoplanin-PE, CD31-PECy7, EpCAM-FITC, CD64-BV711 (Biolegend), CD11c-PECy5.5 (Invitrogen) CXCR3-PECy7, CD3-Alexa700 and Ly6C-efluor450 (eBioscience). For subsequent detection of CXCL10 mRNA Primeflow^® RNA Assay (eBioscience) was used accordingly to manufacture protocol. Samples were acquired on an LSR-II flow cytometer (BD Biosciences) and analysed using FlowJo software (Tree Star Inc.).
Single cell suspension isolated from human tumors and unaffected colon tissue were stained with Live/dead Aqua (Molecular Probes, Inc.), CD31-Alexa700, (Biolegend), CD4-PerCP, CD8-BV711, CD105-APC, CD14-Alexa700, CD19-APCH7 and CD19-PE-CF594 (BD bioscience) followed by permeabilization with Fix & Perm kit (ADG Bio research GMBH) and staining with CXCL9-FITC (R&D) and CXCL10-PE (Biolegend), flow cytometry analyses were performed as above.

Akeus P., Szeponik L., Ahlmanner F., Sundström P., Alsén S., Gustavsson B., Sparwasser T., Raghavan S, & Quiding-Järbrink M. (2018). Regulatory T cells control endothelial chemokine production and migration of T cells into intestinal tumors of APCmin/+ mice. Cancer Immunology, Immunotherapy, 67(7), 1067-1077.

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