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Tru nuclear transcription buffer set

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The Tru-nuclear Transcription Buffer set is a laboratory product designed for the extraction and purification of nuclear proteins, including transcription factors, from cell samples. The set includes reagents for cell lysis, nuclear protein extraction, and buffer preparation to facilitate downstream nuclear protein analysis and applications.

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Lab products found in correlation

Rabbit igg isotype control, by Thermo Fisher Scientific (2 mentions) Facsdiva v6, by BD (2 mentions) Cd279 pd 1, by BioLegend (2 mentions)

Spelling variants of this product

Tru-Nuclear Transcription Factor Buffer Set (2 citations)

2 protocols using tru nuclear transcription buffer set

1

Multiparametric Immunophenotyping of PBMCs

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PBMCs were isolated as described above and stained with the following fluorochrome-conjugated antibodies: CD19, CD27, CD38, IgD (BD Biosciences), CD11c, CD3, CXCR5, and CD279 (PD-1) (BioLegend) in the presence of 10% normal mouse serum. For intracellular staining, cells were fixed, permeabilized and stained using the Tru-nuclear Transcription Buffer set (BioLegend). Cells were blocked with 50 μg/ml Rabbit IgG and stained with 2 μg/ml rabbit anti-human ATF3 (USBIO) or Rabbit IgG isotype control (Invitrogen). Single color stained anti-mouse Ig beads (Bangs) were used as compensation controls. Flow cytometry were collected with BD FACSDiva v6.2 and data analysis was performed FlowJo (TreeStar) v10.4.
Scharer C.D., Blalock E.L., Mi T., Barwick B.G., Jenks S.A., Deguchi T., Cashman K.S., Neary B.E., Patterson D.G., Hicks S.L., Khosroshahi A., Lee F.E., Wei C., Sanz I, & Boss J.M. (2019). Epigenetic programming underpins B cell dysfunction in human SLE. Nature immunology, 20(8), 1071-1082.
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2

Multiparametric Immunophenotyping of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were isolated as described above and stained with the following fluorochrome-conjugated antibodies: CD19, CD27, CD38, IgD (BD Biosciences), CD11c, CD3, CXCR5, and CD279 (PD-1) (BioLegend) in the presence of 10% normal mouse serum. For intracellular staining, cells were fixed, permeabilized and stained using the Tru-nuclear Transcription Buffer set (BioLegend). Cells were blocked with 50 μg/ml Rabbit IgG and stained with 2 μg/ml rabbit anti-human ATF3 (USBIO) or Rabbit IgG isotype control (Invitrogen). Single color stained anti-mouse Ig beads (Bangs) were used as compensation controls. Flow cytometry were collected with BD FACSDiva v6.2 and data analysis was performed FlowJo (TreeStar) v10.4.
Scharer C.D., Blalock E.L., Mi T., Barwick B.G., Jenks S.A., Deguchi T., Cashman K.S., Neary B.E., Patterson D.G., Hicks S.L., Khosroshahi A., Lee F.E., Wei C., Sanz I, & Boss J.M. (2019). Epigenetic programming underpins B cell dysfunction in human SLE. Nature immunology, 20(8), 1071-1082.
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