To confirm depletion of basophil IL-18R (Supplemental Fig. 1 ), we collected spleens from basoIL-18R (−) mice (n = 3) and basoIL-18R (+) mice (n = 3) at 4 d PI for flow cytometry. Spleens were prepared as described previously (9 (link)). In brief, 107 cells per spleen were incubated with anti-mouse CD49b (PerCP) (eBioscience), anti-mouse FCεR1α (allophycocyanin) (eBioscience), and anti-mouse IL-18R (PE) (eBioscience) at the manufacturer’s recommended concentrations for 1 h at room temperature, protected from light, then stained with DAPI (Invitrogen, Waltham, MA). Cells were counted using a CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA). Dead cells were excluded based on DAPI staining, and basophils within this population were defined as expressing both CD49b and FCεR1α (19 (link), 20 (link)). Data were analyzed with CytExpert Software (Beckman Coulter).
Anti mouse il 18r pe
Manufactured by Thermo Fisher Scientific
Anti-mouse IL-18R (PE) is a fluorescently labeled antibody specific for the mouse interleukin-18 receptor. It is designed for use in flow cytometry applications to detect and analyze cells expressing the IL-18 receptor.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Cytexpert software, by Beckman Coulter (2 mentions)
Cytoflex flow cytometer, by Beckman Coulter (2 mentions)
Anti mouse cd49b percp, by Thermo Fisher Scientific (2 mentions)
Anti mouse fcεr1α allophycocyanin, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti mouse il 18r pe
1
Basophil IL-18R Depletion Quantification
2
Basophil IL-18R Depletion Quantification
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To confirm depletion of basophil IL-18R (Supplemental Fig. 1 ), we collected spleens from basoIL-18R (−) mice (n = 3) and basoIL-18R (+) mice (n = 3) at 4 d PI for flow cytometry. Spleens were prepared as described previously (9 (link)). In brief, 107 cells per spleen were incubated with anti-mouse CD49b (PerCP) (eBioscience), anti-mouse FCεR1α (allophycocyanin) (eBioscience), and anti-mouse IL-18R (PE) (eBioscience) at the manufacturer’s recommended concentrations for 1 h at room temperature, protected from light, then stained with DAPI (Invitrogen, Waltham, MA). Cells were counted using a CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA). Dead cells were excluded based on DAPI staining, and basophils within this population were defined as expressing both CD49b and FCεR1α (19 (link), 20 (link)). Data were analyzed with CytExpert Software (Beckman Coulter).
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