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13 protocols using v 770 uv vis nir spectrophotometer

1

Film Blends Release Kinetics

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Rectangular samples of film blends (24 mm2, 6.5 mg, 120–150 µm) containing ILs at concentrations of 0.5, 1, and 5 wt% were immersed in 3 mL of PBS and kept at 37 °C for 24 h. A Jasco V-770 UV–vis–NIR spectrophotometer (Jasco Europe S.R.L., Cremella, LC, Italy) was used to determine the release kinetics. Aliquots were collected at specific interval times (1–24 h), and the optical absorption at 210 nm was measured. Experiments were performed in triplicates, and data are presented as the mean ± S.D.
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2

Characterization of Perovskite Films and Devices

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Absorption spectra of perovskite films were tested using a V-770 UV/VIS/NIR spectrophotometer (Jasco, Japan). Use a fluorescence spectrophotometer model Hitachi F-7000 (Tokyo, Japan) to test the photoluminescence (PL) spectrum of perovskite films. X-ray diffraction (XRD) patterns of perovskite films were tested using a PANalytical X’Pert PRO MRD diffractometer (Almelo, The Netherlands) using CuKα (λ = 1.5418 Å) radiation source. The surface morphologies and high-resolution transmission electron microscopy (TEM) image of the perovskite films and PeQDs were observed using a ZEISS Sigma field emission scanning electron microscope (FESEM) instrument (ZEISS, Germany) and a JEM-2100F transmission electron microscope instrument (JEOL, Japan), respectively. The photocurrent–voltage (J–V) curves of the PeQDs/triple-cation PeSCs were obtained by using a MFS-PV-Basic solar simulator (Hong-Ming Technology Co., Ltd., Taiwan) with a Keithley 2420 source meter under illumination of AM 1.5G simulated sunlight at 100 mW cm−2, calibrated by a NREL PVM-894 standard silicon reference cell (PV Measurements Inc., USA). External quantum efficiency (EQE) was tested by LSQE-R QE system (LiveStrong Optoelectronics Co., Ltd., Taiwan) equipped with a LAMBDA 35 UV–VIS-NIR spectrophotometer (PerkinElmer, USA) and a XES-204S 150 W xenon lamp (San-Ei Electric Co., Ltd., Japan) as a light source.
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3

Determining Optical Properties of Glasses

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The thickness and refractive indices of the prepared glasses play an important role in the estimation of luminescence parameters. So, the refractive indices (n) were determined by the Abbe's refractometer using sodium vapour lamp as a source. The optical absorption spectra were recorded using Jasco V-770 UV-VIS-NIR spectrophotometer. The luminescence and decay measurements were performed using FLS-980 fluorescence spectrometer with xenon flash lamp as a source. All the spectral measurements were carried out at room temperature.
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4

Characterization of Biomolecular Interactions

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Absorption measurements were performed using
a JASCO V-770 UV–vis–NIR spectrophotometer. NMR measurements
were performed using a Bruker 400 MHz NEO 5 mm BBFO probe. ITC experiments
were performed using a NanoITC calorimeter (TA Instruments) equipped
with a 1 mL Hastelloy sample and reference cells with a 250 μL
syringe. Thermogravimetric analysis (TGA) measurements were performed
using a TGA-5500 (TA Instruments). Attenuated total reflection Fourier
transform infrared (ATR-FTIR) measurements were performed using a
Nicolet iS50 FT-IR spectrometer with Smart iTX accessory (Thermo Scientific).
ITC curves fitting and conformational entropy calculations were done
using MATLAB (The MathWorks Inc.).
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5

Adsorption Studies of CeO2 for Congo Red Removal

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Adsorption studies were carried out on all four synthesized CeO2 samples (1 g L−1) using Congo red as the model pollutant at a concentration of 20 mg L−1. The adsorption experiments were carried out in magnetically stirred glass vessels at the ambient pH of the Congo red solution. At regular contact intervals, samples were withdrawn, centrifuged and analysed using a Jasco V-770 UV-vis-NIR spectrophotometer. To evaluate the selectivity, adsorption analyses were performed with methylene blue and methyl orange also. The effect of dye concentration and pH on adsorption activity was evaluated by varying the initial dye concentrations (10, 15, 20, 25 and 30 mg L−1) and by carrying out adsorption studies under 3 different pH conditions (3, 6.5 (ambient pH) and 10). Besides this, the pH of the point of zero charge (pHPZC) of the adsorbent material was determined using the pH drift method.45 (link)
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6

Optical Characterization of PbS/CdS Quantum Dots

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QDs were precipitated with hexane:ethanol (1:3) and resuspended in TCE for optical characterization; TCE was chosen to minimize solvent absorbance in the SWIR.13 (link),45 (link) Absorbance spectra of the QDs were measured with a Jasco V-770 UV-Vis-NIR spectrophotometer. Photoluminescence (PL) spectra of the core/shell QDs were recorded with a FLS1000 photoluminescence spectrometer (Edinburgh Instruments, UK) equipped with a near infrared photomultiplier tube (NIR-PMT) with sensitivity up to 1700 nm. The same spectrometer was also used to measure the absolute quantum yield (QY) values of the PbS/CdS QDs. Cuvettes containing dilute QD samples (~0.5 abs at excitation) in TCE were placed in an integrating sphere and excited with a Xenon arc lamp excitation source with 10 nm slitwidth at 808 nm, selected to match the excitation wavelength used in the imaging studies. With pure TCE in the same sample holder used in the background scan, the absolute QY was calculated with Fluoracle® (Edinburgh Instruments, UK) software.
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7

Measuring Absorption Spectra of Compounds

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Absorption spectra were acquired on a JASCO V-770 UV-VIS/NIR spectrophotometer with a 2,000 nm/min scan rate after blanking with the appropriate solvent in quartz cuvettes (10 mm for measurements in EtOH, water, FBS, and micelles in water, 0.2 mm for measurements in whole blood).
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8

Tracking Gelatin-Coated Gold Nanoparticles

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The gelatin@Au-NRs were dispersed in complete cell culture medium DMEM (Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum containing 1% L-glutamine-penicillin-streptomycin and 0.5% Geneticin G418) (Sigma, Kanagawa, Japan) and PBS (Ca2+ and Mg2+ free, pH 7.4) (Sigma, Japan) in a cuvette holder. The changes in absorption spectra with time were monitored using a JASCO V-770 UV-Vis-NIR spectrophotometer.
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9

Optical Characterization of Thin Films

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A DXR micro-Raman spectrometer was used for a backscattering geometry of the sample from ThermoFisher Scientific with a diode laser of 532 nm (2.33 eV). Ultraviolet-visible (UV-vis) absorption measurements of the thin films were recorded using a single monochromator V-770 UV-vis-NIR spectrophotometer from Jasco in the wavelength range of 200–800 nm. Room temperature photoluminescence (RT-PL) measurements were performed with a Perkin Elmer Spectrofluorometer LS55 (Xenon source).
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10

Comprehensive Characterization of Novel Materials

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Powder diffractograms
were recorded on a Bruker AXS D8 ADVANCE diffractometer in the Bragg–Brentano
geometry, with Cu Kα1 radiation (0.15406 nm) in a 2Θ range
from 6 to 60°. TEM analysis was performed with a Hitachi HT7700
transmission electron microscope, at 100 kV accelerating voltage.
The FT-IR spectra were recorded for the samples mixed with KBr and
pressed into disks using a JASCO FT/IR-4200 spectrometer in the range
from 4000 to 400 cm–1. Luminescence measurements
were performed with a Hitachi F-7000 spectrofluorometer at ambient
conditions, and the spectra were corrected for the apparatus response.
The optical absorption spectra of all the samples were recorded in
a diffuse reflectance mode with a JASCO V770 UV–vis–NIR
spectrophotometer equipped with a spherical integrator (150 mm in
diameter). DLS measurements were performed using a Malvern Zetasizer
Nano ZS Instrument.
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