Cd138 apc 281 2

Manufactured by BD

CD138-APC (281-2) is a monoclonal antibody conjugated with the fluorescent dye Allophycocyanin (APC). It is designed for the identification and enumeration of CD138-positive cells using flow cytometry.

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Lab products found in correlation

Propidium iodide staining, by Merck Group (1 mentions) Cyan adp analyzer, by Beckman Coulter (1 mentions) Cd138 pe 281 2, by BD (1 mentions) B220 ra3 6b2 apc, by Thermo Fisher Scientific (1 mentions) Cd3 apc cy7 17a2, by BioLegend (1 mentions) B220 pe ra3 6b2, by BioLegend (1 mentions) Cd19 bv605 6d5, by BioLegend (1 mentions) Cd19 pe 6d5, by BioLegend (1 mentions) Cd4 apc cy7 gk1.5, by BioLegend (1 mentions) Cd8 percpcy5.5 53 6 7, by BioLegend (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Streptavidin bv605, by BioLegend (1 mentions) Protein labeling kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD138-APC (19 citations) CD138 (281-2, (6 citations) CD138-APC (clone 281–2; (2 citations) APC-conjugated anti- CD138 (281–2, (2 citations) APC-conjugated anti-CD138 (clone 281-2) (2 citations)

2 protocols using cd138 apc 281 2

Immunophenotyping and Proliferation Analysis

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Cells stimulated with ALD-DNA, LPS, or a combination of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience), CD86-PE (GL1, eBioscience), MHC class II-FITC (2G9, BD Biosciences), or CD138-PE (281-2, BD Biosciences). CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences). Appropriate isotype-matched antibodies were included as controls. Data were acquired on a CyAn ADP Analyzer and analyzed using Summit 5.2 software (both from Beckman-coulter). Dead cells and debris were excluded by forward scatter and side scatter (FSC/SSC) gating and propidium iodide staining (Sigma-Aldrich). For BrdU incorporation assay using a FITC BrdU Flow kit, cells were fixed, permeabilized, and treated with DNase prior to BrdU staining using a FITC-coupled anti-BrdU monoclonal antibody, as recommended by the manufacturer. The incorporated BrdU was measured in conjunction with DNA content by 7-AAD staining.

Lu Q., Wang J.Y., Wang L., Jiang X, & Chu Y. (2014). Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production. PLoS ONE, 9(10), e109095.

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Comprehensive Multiparameter Flow Cytometry

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The following anti-mouse antibodies were used for FACS: CD19-PE (6D5), CD19-BV605 (6D5), CD3-AlexaFluor700 (17A2), CD3-APCCY7 (17A2), CD4-APC-Cy7 (GK1.5), CD8-PerCP-Cy5.5 (53-6.7), CD8-FITC (5H10-1), Streptavidin BV605, PD-1-BV421 (29F.1A12), PDL-1-Pecy7 (10F.9G2), Bcl6-PE-Dazzle594 (7D1), ICOS-AlexaFluor488 (C398.4A), CD45.1- BV605 (A20), B220-APCCy7 (RA3-6B2), Live Dead Aqua 430 were from Biolegend; CD19-AlexaFluor700 (1D3), CD19-FITC (1D3), IgD-FITC (11-26c), CD8-PECy7 (53-6.7), TNF-APC (MP6-XT22), PD-L1-PE (MIH5) and were from eBioscience; CD138-BV605 (281-2), CD138-APC (281-2), CD138-PE (281-2), CD138- Biotin, CD38-BV421 (90/CD38), CXCR5-Biotin (2G8), IFN-γ-FITC (XMG1.2), Ki-67-AlexaFluor647 (B56), Blimp-1-PE-CF594 (5E7), and CD8-BUV805 (53-6.7) were from BD Biosciences.
The following anti-mouse antibodies were used for tissue immunofluorescence: B220-PE (RA3-6B2) and CD3 FITC anti-CD3 (145-2C11) from Biolegend and CD138-APC (281-2) from BD Biosciences.
HEL was conjugated with AlexaFluor647 with a protein labeling kit (Invitrogen).

Serrán M.G., Vernengo F.F., Almada L., Beccaria C.G., Gazzoni Y., Canete P.F., Roco J.A., Boari J.T., Ramello M.C., Wehrens E., Cai Y., Zuniga E.I., Montes C.L., Cockburn I.A., Rodriguez E.V., Vinuesa C.G, & Gruppi A. (2022). Extrafollicular Plasmablasts Present in the Acute Phase of Infections Express High Levels of PD-L1 and Are Able to Limit T Cell Response. Frontiers in Immunology, 13, 828734.

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