Zetasizer nano ns model dts 1060

Commercial AgNPs formulation, Argovit^®, was obtained from Scientific-Production Centre Vector-Vita Ltd., Novosibirsk, Russia. Argovit^® is a water suspension of AgNPs with an average size of 38 ± 15 nm coated with polyvinylpyrrolidone (PVP). The supplier’s specifications indicate a metallic content of 12 mg/mL with 188 mg/mL of coating agent to generate a 20% AgNPs (200 mg/mL) suspension. AgNPs characterization was performed by Transmission Electron Microscopy (TEM, JEOL JEM-2010, Tokyo, Japan) and silver content determined by Inductively coupled plasma-optical emission spectroscopy (ICP-OES, Varian, Palo Alto, CA, USA) before using. Z-potential was determined in a Malvern Instruments Zetasizer Nano NS model DTS 1060 (Malvern Instruments, Worcestershire, UK) in triplicate.

Dynamic light scattering (DLS) (Malvern Instruments Zetasizer Nano NS model DTS1060; λ = 532 nm) was used to determine the hydrodynamic diameter and the zeta potential. Characterization of optical properties was done with the Cary 60 UV-vis spectrophotometer (Agilent Technologies) in the range of 200 to 900 nm. Morphology and size distribution were determined by HR-TEM using a JEOL JEM-2010 microscope. Also, lyophilized AgNPs were characterized by FTIR-ATR in a range of 400 to 4000 cm⁻¹ with a resolution of 2 cm⁻¹ on a universal diamond ATR Top Plate accessory (PerkinElmer). The sample spectrum was compared with that of standard solid PVP (MW 100 kDa). The silver content of Argovit® was determined by ICP-OES (Varian Vista-MPX CCD Simultaneous ICP-OES).