Anti dbf4

Manufactured by Abcam

Anti-Dbf4 is a primary antibody that recognizes the Dbf4 protein. Dbf4 is a regulatory subunit of the Cdc7 kinase, which is essential for the initiation of DNA replication. The antibody can be used to detect and study the expression and localization of Dbf4 in various cell and tissue samples.

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Lab products found in correlation

Anti human cd3, by BD (1 mentions) Anti mouse cd4, by BD (1 mentions) Anti mouse cd4, by BioLegend (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti erk, by BD (1 mentions) Anti human cd8, by Thermo Fisher Scientific (1 mentions) Anti mouse cd4, by Thermo Fisher Scientific (1 mentions) Anti human cd8, by BD (1 mentions) Anti rna polymerase 2, by Abcam (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions) Anti mouse cd8, by BD (1 mentions) Anti mouse cd25, by BD (1 mentions) Anti mouse tcrβ, by BD (1 mentions) Anti mouse ifn γ, by BioLegend (1 mentions) Anti human cd11a, by BioLegend (1 mentions) Anti mouse cd69, by Thermo Fisher Scientific (1 mentions) Anti mouse il 2, by Thermo Fisher Scientific (1 mentions) Anti rna polymerase 2 phospho s2, by Abcam (1 mentions) Anti gapdh, by BioLegend (1 mentions) Anti pplcγ, by Cell Signaling Technology (1 mentions)

2 protocols using anti dbf4

Detailed Immunological Antibody Protocols

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The antibodies used in this study for flow cytometry include: anti-mouse CD8 (#563786), anti-mouse CD25 (#558642), anti-mouse TCRβ (#553174), anti-human CD3 (#563798), anti-human CD8 (#563795), and anti-human TCRαβ (#563826) from BD Biosciences; anti-mouse CD4 (#100544), anti-mouse IFN-γ (#505835), anti-human LFA-1 (#363404), anti-human CD11a (#301208), anti-human CD18 (#302114), anti-human CD25 (#302625), and anti-human CD69 (#310920) from BioLegend; anti-mouse CD4 (#17-0042-83), anti-mouse CD4 (#17-0041-83), anti-mouse CD69 (#25-0691-82), anti-mouse TNF (#17-7321-82), anti-mouse IL-2 (#12-7021-82), anti-human CD4 (#17-0048-2), and anti-human CD8 (#11-0088-41) from eBioscience.
The antibodies used for immunoprecipitation and immunoblotting include: anti-Cdc7 (#ab10535), anti-Dbf4 (#ab124707), anti-RNA polymerase II (#ab817) and anti-RNA polymerase II (phospho S2, # ab193468) from Abcam; anti-PLCγ (#610027) and anti-ERK (#610031) from BD Biosciences; anti-LAT (#641102) from BioLegend; anti-GAPDH (#2118), anti-pPLCγ (#2821), anti-ZAP70 (#2709), anti-pZAP70 (#2717), anti-pERK (#4370), anti-pLAT (#3584), anti-pLck (#6943), and anti-NF-κB (#3035) from Cell Signaling; anti pMCM2 S40 (#GTX62847) from GeneTex; anti-MCM2 (#MA5-15895) from Pierce; and anti-Lck (#sc-433) from Santa Cruz.

Chen E.W., Tay N.Q., Brzostek J., Gascoigne N.R, & Rybakin V. (2019). A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Frontiers in Immunology, 10, 1718.

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Immunoblotting of Cell Signaling Proteins

Cited 1 time

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Immunoblotting was performed as described previously (44 (link)). The following antibodies were used at 0.1 to 0.5 μg/ml: anti-pMCM2 (3378-1; Epitomics Inc.), anti-MCM2 (sc-9839; Santa Cruz Biotechnology), pChk1 (#2348; Cell Signaling Technology), anti-Chk1 (#2345; Cell Signaling Technology), anti-pCDK1 (#9111; Cell Signaling Technology), anti-CDK1 (sc-954; Santa Cruz Biotechnology), anti-pBRCA1 (#9009; Cell Signaling Technology), anti-pp53 (#9286; Cell Signaling Technology), anti-pATM (2152-1; Epitomics Inc.), anti-p21 (#2947; Cell Signaling Technology), anti-gH2AX (#2577; Cell Signaling Technology), anti-p53 (sc-126; Santa Cruz Biotechnology), anti-PARP1 (#9542; Cell Signaling Technology), anti-BRCA1 (sc-6954; Santa Cruz Biotechnology), anti-CDC7 (sc-56274; Santa Cruz Biotechnology), anti-DBF4 (ab124707; Abcam), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (MAB374; Chemicon). Immunoblotted proteins were visualized using chemiluminescence.

Iwai K., Nambu T., Kashima Y., Yu J., Eng K., Miyamoto K., Kakoi K., Gotou M., Takeuchi T., Kogame A., Sappal J., Murai S., Haeno H., Kageyama S.I., Kurasawa O., Niu H., Kannan K, & Ohashi A. (2021). A CDC7 inhibitor sensitizes DNA-damaging chemotherapies by suppressing homologous recombination repair to delay DNA damage recovery. Science Advances, 7(21), eabf0197.

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