Ascend 3 hd 600 mhz spectrometer

Nuclear magnetic resonance experiments were performed on a Bruker Ascend III HD 600 MHz spectrometer equipped with a 5 mm cryoprobe -TCI with an effective resolution of 900 MHz (Universidad de Antioquia, Colombia). The mono and bidimensional experiments were stored using the standard pulse sequences of the equipment. Chemical shifts (δ) are expressed in ppm and the data analysis was carried out using MestReNova version 11.0 (Mestrelab Research, Santiago de Compostela, Spain). Mass spectra were obtained in a SQD2 detector coupled to a UPLC H-class chromatographic system in positive and negative modes and data analysis was performed using Waters MassLynx™ software Version 4.1 (Waters, Milford, MA, USA). The elemental composition and high-resolution measurement were carried out on a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).

All ¹H NMR experiments were recorded at 300.0 K (±0.05) using a Bruker Ascend III HD 600 MHz spectrometer coupled with a 5 mm TCI cryoprobe (Bruker-Biospin, Billerica, MA, USA). Using a randomized running order, 1D ₁H NMR spectra were acquired using a pulse sequence developed by Ravanbakhsh et al. [21 (link)]. More information can be found in the Supplementary Information.