Nupage novex 4 12 bis tris precast polyacrylamide gels

Manufactured by Thermo Fisher Scientific

Sourced in United States

The NuPAGE Novex 4–12% Bis-Tris precast polyacrylamide gels are laboratory equipment used for protein separation and analysis in electrophoresis applications. They are pre-cast gels that provide consistent and reliable results.

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Lab products found in correlation

Phospho akt ser505, by Cell Signaling Technology (1 mentions) Lds loading buffer, by Thermo Fisher Scientific (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Image lab software, by Bio-Rad (1 mentions) Phosstop phosphatase inhibitor cocktail, by Roche (1 mentions) Iblot transfer stack, by Thermo Fisher Scientific (1 mentions) Iblot gel transfer device, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

NuPAGE Novex 4-12% Bis-Tris precast gels NuPAGE Novex 4–12% Bis-Tris polyacrylamide gels Novex Bis-Tris precast gel 4%–12%; NuPage precast 4–12% Bis-Tris gels NuPage 4–12% Bis-Tris precast gels NuPAGE Novex 4–12% Bis-Tris gels NuPAGE 4–12% Bis-Tris polyacrylamide gels NuPAGE Novex Bis-Tris precast gel NuPAGE Bis-Tris precast polyacrylamide gels NuPAGE Novex Bis-Tris polyacrylamide gels

2 protocols using nupage novex 4 12 bis tris precast polyacrylamide gels

Western Blot Analysis of Fly Protein

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Antibodies for Akt (#9272), phospho-Akt (Ser505) (#4054), non-phospho-4E-BP1 (Thr46) (#4923), phospho-4E-BP1 (Thr37/46) (#2855) and β-actin (#4967) were purchased from Cell Signaling Technology (Danvers, MA, USA). Ten flies were homogenized in 100 μl RIPA buffer with protease inhibitor cocktail (Sigma, St. Louis, MO, USA) and PhosSTOP phosphatase inhibitor cocktail (Roche, Nutley, NJ, USA). Supernatant was incubated with LDS loading buffer (Invitrogen, Grand Island, NY, USA) at 70°C for 10 min. Thirty micrograms of denatured protein was separated on 10% NuPAGE Novex 4–12% Bis-Tris precast polyacrylamide gels (Invitrogen, Grand Island, NY, USA) and transferred to PVDF membranes. Following incubation with primary and secondary antibodies, blots were visualized with Pierce ECL western blotting substrate (Thermo Fisher Scientific, Waltham, MA, USA). Three independent biological replicates were generated for all analyses, and samples for AKT and 4E-BP produced at different times. Band intensity was quantified with Image Lab software (Bio-Rad, Hercules, CA, USA).

Bai H., Post S., Kang P, & Tatar M. (2015). Drosophila Longevity Assurance Conferred by Reduced Insulin Receptor Substrate Chico Partially Requires d4eBP. PLoS ONE, 10(8), e0134415.

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Rapid Protein Transfer Optimization

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SDS–PAGE was performed on NuPAGE® Novex® 4-12% Bis-Tris pre-cast polyacrylamide gels (Invitrogen). We used iBlot® Transfer Stacks and the iBlot® Gel Transfer Device (Thermo Fisher Scientific) for protein transfer. The 7-min transfer programme efficiently transferred most of the proteins.

Galvan L., Francelle L., Gaillard M.C., de Longprez L., Carrillo-de Sauvage M.A., Liot G., Cambon K., Stimmer L., Luccantoni S., Flament J., Valette J., de Chaldée M., Auregan G., Guillermier M., Joséphine C., Petit F., Jan C., Jarrige M., Dufour N., Bonvento G., Humbert S., Saudou F., Hantraye P., Merienne K., Bemelmans A.P., Perrier A.L., Déglon N, & Brouillet E. (2018). The striatal kinase DCLK3 produces neuroprotection against mutant huntingtin. Brain, 141(5), 1434-1454.

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