Mice were fasted for 16 h prior to glucose measurement and plasma collection. Glucose concentrations were measured using glucose strips and a glucometer (Lifescan One Touch Ultra). Insulin levels were measured using a mouse ultrasensitive insulin ELISA kit (Crystal Chem, 90080). Serum triglycerides of fasted mice and liver triglycerides from fed mice were measured using a triglyceride assay kit (Cayman Chemical, 10010303). GTT was performed in 16-h-fasted mice using 1.5 g/kg glucose interperitoneal injection. Serum alanine aminotransferase measurements were performed by IDEXX BioResearch Laboratory.
Triglyceride assay kit
Manufactured by Cayman Chemical
Sourced in United States
The Triglyceride assay kit is a laboratory tool used to quantify the concentration of triglycerides in biological samples. It provides a standardized method for the enzymatic determination of triglyceride levels.
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Lab products found in correlation
Total cholesterol assay kit, by Cell Biolabs (3 mentions)
Amplex red cholesterol assay kit, by Thermo Fisher Scientific (3 mentions)
Mouse ultrasensitive insulin elisa kit, by Crystal Chem (1 mentions)
Onetouch ultra, by LifeScan (1 mentions)
Standard diluent, by Cayman Chemical (1 mentions)
Enzyme immunoassay kit, by Cayman Chemical (1 mentions)
Cholesterol fluorometric assay kit, by Cayman Chemical (1 mentions)
Au680 automatic analyzer, by Beckman Coulter (1 mentions)
Ldh assay kit, by Merck Group (1 mentions)
Cobas c111 analyser, by Roche (1 mentions)
Kx 21, by Sysmex (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Elisa microplate reader, by Harvard Bioscience (1 mentions)
Amplex red cholesterol assay, by Thermo Fisher Scientific (1 mentions)
Tandem superose 6 columns, by GE Healthcare (1 mentions)
Mouse insulin enzyme linked immunosorbent assay kit, by ALPCO (1 mentions)
Glucose assay kit, by Cayman Chemical (1 mentions)
Alt activity assay kit, by Cayman Chemical (1 mentions)
Rt pcr reagent, by Thermo Fisher Scientific (1 mentions)
29 protocols using triglyceride assay kit
1
Metabolic Profiling in Fasted Mice
2
Adipocyte Triglyceride Quantification
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Tumor cells were grown in transwell with adipocytes in the presence or absence of 10 μM Atglistatin. After 48 hours, adipocytes were collected as previously indicated and re-suspended in the Standard Diluent (provided in the Triglyceride assay kit; Cayman Chemical). Samples were sonicated and centrifuged. The supernatant was then used for the assay. All steps were performed according to manufacturer's protocol. Experiments were done in triplicate and reported as mean ± SD.
3
Enzymatic Plasma Lipid Quantification
4
Quantitative Assessment of Plasma Lipid Profile and Oxidative Stress Markers
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The plasma total triglyceride (TG) content was determined quantitatively by a colorimetric (at 570 nm) Triglyceride Assay kit (Cayman Chemical, Ann Arbor, MI) according to instructions. Plasma cholesterol levels were determined by a Cholesterol Fluorometric Assay kit (Cayman Chemical), and total cholesterol content for each sample was estimated on the basis of relative fluorescence (excitation 550 nm, emission 590) obtained from standards. Plasma 8-isoprostane levels at 18 weeks were determined by a Cayman Enzyme Immunoassay Kit as described [8 (link), 17 (link)–19 (link)]. RBC reduced (GSH) and oxidized (GSSG) glutathione levels were assessed enzymatically by the DTNB-GSSG reductase method [17 (link)–19 (link)]. Plasma 3-nitrotyrosine (3-NT) results, determined by the enzyme immunoassay components from Cayman Chemical, were derived from the concurrent study just published [8 (link)].
5
Liver Lipid Profiling in Mice
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Plasma and lipid analysis was performed as previously described [6] (link). Briefly, liver tissue samples were homogenized in Methanol:Water (1:1 v/v) and then lipids were extracted with Dichloromethane: Isopropanol: Methanol (25:10:65, v/v/v) and analyzed by mass spec against internal standards. Liver triglyceride levels in ACC-DKI mice were measured using a Triglyceride Assay Kit (Cayman Chemical) as previously described [23] (link).
6
Triglyceride Quantification in Liver Tissues
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Liver tissues were incubated on ice in 100 μL of saline solution (2 M NaCl, 2 mM EDTA, 50 mM sodium phosphate, pH 7.4). Tissue suspensions were assayed for TG content using a triglyceride assay kit (Cayman Chemical).
7
Comprehensive Liver Lipid Profiling
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Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL), low-density lipoprotein-cholesterol (LDL), and glucose were measured using an AU680 Automatic Analyzer (Beckman coulter, CA, USA), and lactate dehydrogenase (LDH) was measured using a commercial LDH assay kit (Sigma, St. Louis, MO, USA). Lipid in the liver tissue was extracted using modified methods, as previously described [37 (link)]. Briefly, stored liver tissues were homogenized in chloroform-methanol-distilled water (2 : 1 : 1, v/v/v) using a homogenizer for 1 min, and then centrifuged at 13,000 rpm for 15 min at 4°C. The solvent (chloroform) phase obtained from the extraction was dried and dissolved in methanol, and then the hepatic TG levels were measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI, USA).
8
Comprehensive Blood Analysis Protocol
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Blood samples were collected biweekly and at the final time points by retrobulbar sinus puncture. Red blood cell, white blood cell and blood platelet counts, as well as haemoglobin and haematocrit, were assessed with an automated cell counter (Sysmex KX-21, Sysmex Deutschland GmbH, Norderstedt, Germany). The activities of alanine aminotransferase (ALT) and GLDH in ethylenediaminetetraacetic acid (EDTA)-treated plasma were measured spectrophotometrically as indicators of hepatocellular disintegration and necrosis using a cobas c 111 analyser (Roche Diagnostics, Rotkreuz, Switzerland) according to the manufacturer’s instructions. Plasma triglycerides were measured using a triglyceride assay kit (10010303, Cayman Chemical Company, Ann Arbor, Michigan, USA) according to the manufacturer’s instructions.
9
Hepatic Triglyceride Quantification
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Triglyceride in liver was measured according to the manufacturer's instruction using the triglyceride assay kit from Cayman.
10
Cellular Cholesterol and Triglyceride Quantification
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