Fab170p

Manufactured by R&D Systems

The FAB170P is a high-quality laboratory centrifuge designed for general purpose applications. It features a maximum speed of 17,000 RPM and a maximum RCF of 24,600 x g. The centrifuge accommodates a variety of rotor options to handle different sample volumes and tube sizes. The FAB170P is a reliable and versatile laboratory equipment piece for researchers and scientists.

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Lab products found in correlation

Ic002p, by R&D Systems (2 mentions) Fab332a, by R&D Systems (1 mentions) Ic002a, by R&D Systems (1 mentions) Facscalibur platform, by BD (1 mentions) Ab34139, by Abcam (1 mentions) Ic002f, by R&D Systems (1 mentions) Gtx61796, by GeneTex (1 mentions) Gtx124222, by GeneTex (1 mentions) Fab357p, by R&D Systems (1 mentions) Ab1791, by Abcam (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

3 protocols using fab170p

Flow Cytometric Analysis of Stem Cell Markers

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For flow cytometric analysis, the cells were harvested and dissociated into single cells with 0.25% trypsin and then washed in PBS plus 0.5% BSA twice. After washing, the cells were stained with antibodies to CXCR4 (FAB170P; R&D system), c-Kit (FAB332A; R&D system) and DAZL (ab34139; abcam) for 40 min at 4°C. After washing by PBS twice, the cell pellet was re-suspended in 300 μl PBS for the final flow cytometric analysis of CXCR4 and c-Kit. Besides, the second antibody (ZF-0511, 1:400, Alexa Fluor 488-conjugated goat anti-rabbit IgG, ZSGB-BIO) was added into DAZL treatment for 40 min at 4°C. After that, the cells were washed twice with PBS and re-suspended in 300 μl of PBS for the final flow cytometric analysis. As a control for the analysis, the undifferentiated cells in a separate tube were treated with a mouse IgG₁ APC-conjugated antibody (IC002A; R&D system) and a mouse IgG₁ isotype control-PE (IC002P; R&D system) for c-Kit and CXCR4, respectively. For DAZL, the undifferentiated cells were only strained with the second antibody. Finally, cells analysis was performed on the Becton-Dickinson FACS Calibur platform.

Cheng T., Zhai K., Chang Y., Yao G., He J., Wang F., Kong H., Xin H., Wang H., Jin M., Gong B., Gu L., Yang Z., Wu Y., Ji G, & Sun Y. (2016). CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells. Oncotarget, 8(5), 7814-7826.

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Antibody Utilization for Western Blot, IF, and ChIP

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The following commercially available antibodies were used at the indicated concentrations for Western blot (WB) immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) analyses: JMJD3 (GTX124222, GeneTex, 1:250 IF, 1:500 WB); H3K27me3 (GTX121184, GeneTex, 1:1000 WB, 1–2 μg/ChIP); histone H3 (AB1791, Abcam, 1–2 μg/ChIP); β-actin (A2228, Sigma, 1:2000 WB); lamin A/C (GTX101126, GeneTex, 1:400 IF); γH2AX (GTX61796, GeneTex, 1:400 IF); and Alexa Fluor 488 Phalloidin (A12379, Molecular Probes, 1:40).
The following commercially available antibodies were used at the indicated dilutions for flow cytometry (FC) analyses: VEGFR-PE (FAB357P, R&D Systems, 1:50); IL-6Rα-PE (352803, BioLegend, 1:50); CXCR4-PE (FAB170P, R&D Systems, 1:50); uPAR-FITC (3936CJ, American Diagnostics, 1:50); CXCR1-Alexa647 (335201, BioLegend, 1:50); CXCR2-PE (32075, BioLegend, 1:50); CCR1-PE (335201, BioLegend, 1:50); CCR3-PE (310705, BioLegend, 1:50); CCR5-FITC (313705, BioLegend, 1:50); GM-CSFa-FITC (306906, BioLegend, 1:50); IgG-PE (IC002P, R&D Systems, 1:50); and IgG-fluorescein (IC002F, R&D Systems, 1:50).

Perrigue P.M., Silva M.E., Warden C.D., Feng N.L., Reid M.A., Mota D.J., Joseph L.P., Tian Y.I., Glackin C.A., Gutova M., Najbauer J., Aboody K.S, & Barish M.E. (2015). The Histone Demethylase Jumonji Coordinates Cellular Senescence Including Secretion of Neural Stem Cell-attracting Cytokines. Molecular cancer research : MCR, 13(4), 636-650.

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Quantifying CXCR4, CXCR7, and CXADR Expression

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The breast cancer cell lines were harvested with Versene and washed twice with cold PBS. Cells were incubated at 4°C for 30 minutes to 1 hour with PE-conjugated mouse anti-CXCR4 (FAB170P; R&D Biosystems, Minneapolis, MN) mouse anti-CXCR7 (FAB42271P; R&D Biosystems), or rabbit anti-CXADR (10799-R271-P; Sino Biological Inc, Chesterbrook, PA). PE-conjugated mouse IgG2A antibody (IC003P; R&D Biosystems) or monoclonal rabbit IgG (IC105P; R&D Biosystems) were used as isotype controls. After incubation, the cells were washed twice in cold PBS, then resuspended in 0.5 mL ice-cold PBS and placed on ice. Analysis of receptor expression was conducted via flow cytometry using a FACSCalibur flow cytometer (BD Biosciences).

O’Bryan S.M, & Mathis J.M. (2021). CXCL12 Retargeting of an Oncolytic Adenovirus Vector to the Chemokine CXCR4 and CXCR7 Receptors in Breast Cancer. Journal of cancer therapy, 12(6), 311-336.

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