Nicolet 5700 ftir

Fourier transform infrared spectra of adsorbents were measured by Nicolet 5700 FTIR (Thermo Fisher Scientific, Madison, WI, USA). A 1.0 mg sample and KBr were mixed and pressed in the mass ratio of 1:100. The spectral analysis was carried out at the scanning frequency of 4000 to 400 cm⁻¹. Data were collected and analyzed by Ominic 7.2 software [18 (link)].

UV spectra were obtained on an HP 8453 UV-Vis spectrometer (Hewlett-Packard, Germany) in EtOH solutions (10⁻⁴ mol/L). IR spectra were obtained on a Nicolet 5700 (FT-IR, Thermo Fisher Scientific, Waltham MA, USA) in tablets with potassium bromide. The inorganic components were studied by inductively coupled plasma mass spectrometry using Agilent 7900 JP 14080159 (Agilent Technologies, Tokyo, Japan) using the Speedwave MWS TM-3+ microwave system for organic matrix decomposition. For X-ray diffraction analysis, a Bruker KAPPA APEX II diffractometer (Bruker AXS, Karlsruhe, Germany) equipped with a two-dimensional CCD detector (MoKα radiation with a graphite monochromator, ω-φ scanning) was used.

ECD and absorption spectra were measured in MeOH at room temperature on a J-815 spectropolarimeter (JASCO, Tokyo, Japan), using 1 cm cells. Optical rotations were measured in CHCl₃ on a JASCO P-1010 polarimeter; IR spectra were recorded as deposit glass film on a Thermo Electron Corporation Nicolet 5700 FT-IR (Thermo, Waltham, MA, USA) spectrometer and UV spectra were measured in MeOH on a JASCO V-530 spectrophotometer. ¹H- and ¹³C-NMR spectra were recorded at 400/100 MHz in CDCl₃ on Bruker (Karlsruhe, Germany) spectrometers. The residual peak of the solvent was used as internal standard in each case. Carbon multiplicities were determined by DEPT spectra [22 ]. DEPT, COSY-45, HSQC, HMBC, and NOESY experiments were performed using the Bruker software programs. HRESIMS and ESI spectra were recorded on Shimadzu (Kyoto, Japan) LCMS-IT-TOF Mass Spectrometer and Agilent (Milan, Italy) 6230 Accurate-Mass TOF LC/MS instruments. Analytical and preparative TLC were performed on silica gel (Merck, Darmstadt, Germany, Kieselgel 60, F254, 0.25 and 0.5 mm respectively) plates. The spots were visualized by exposure to UV radiation (253), or iodine vapour. Colum chromatography (CC) was performed using silica gel (Merck, Kieselgel 60, 0.063–0.200 mm).