Immunoblotting was carried out as previously described.10 Antibodies against c‐Ski (#A303‐518A; Bethyl Laboratories, Montgomery, TX, USA), Noggin (4C9; Sigma‐Aldrich), and HA (3F10; Sigma‐Aldrich) were used as primary antibodies. Other primary antibodies and secondary antibodies were prepared as previously described.7 , 8 , 10
Ha 3f10
Manufactured by Merck Group
Sourced in United States
HA (3F10) is a monoclonal antibody produced by Merck Group. It is a laboratory reagent used for research applications. The core function of HA (3F10) is to serve as a detection tool for the identification and quantification of target proteins or other biomolecules in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Ell1 a301 645a, by Fortis Life Sciences (1 mentions)
Enl a302 268a, by Fortis Life Sciences (1 mentions)
Anti aff4 ab57077 antibody, by Abcam (1 mentions)
Ab5392, by Abcam (1 mentions)
Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Myc 9e10, by Santa Cruz Biotechnology (1 mentions)
Nf κb p65 d14e12, by Cell Signaling Technology (1 mentions)
Rangap1, by Abcam (1 mentions)
Anti trfp antibody, by Evrogen (1 mentions)
Anti histone h3, by Abcam (1 mentions)
5 protocols using ha 3f10
1
Immunoblotting Protein Detection Protocol
2
Antibodies for Transcription Regulation
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Polyclonal antibodies against AFF1 (A302-344A), ELL1 (A301-645A), ELL2 (A302-505A), ENL (A302-268A) and AF9 (A300-595A) were purchased from Bethyl Laboratories. Anti-AFF4 (ab57077) antibody was purchased from Abcam. The monoclonal antibodies against Flag (M2) and HA (3F10) were from Sigma-Aldrich and Roche, respectively. The antibodies against CDK9, LARP7 and HEXIM1 were generated in our own laboratory and have been described previously (17 (link),18 (link)).
3
Immunostaining with Fluorescent Antibodies
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The following primary antibodies were used for immunofluorescence imaging and immunoblotting: GFP (3E6; Molecular Probes/Life Tech), GFP (11814460001, clones 7.1 and 13.1; Roche) RFP (600-401-379; Rockland), and HA (3F10; Sigma-Aldrich). Secondary antibodies were high-crossed goat–anti-mouse Alexa Fluor 488 and goat–anti-rabbit, goat–anti-rat Alexa Fluor 568 (Molecular Probes), and goat–anti-rabbit IRdye680TL and goat–anti-mouse IRdyeCW (LI-COR/Westburg).
4
Immunocytochemistry of Neuronal Markers
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Neurons were fixed in 4% PFA/PBS for 10 min, then permeabilized in 0.5% Triton™ X-100/PBS for 5 min. For blocking, 3% bovine serum albumin/PBS was used for 30 min. Primary antibodies were diluted in PBS and incubated at room temperature for 1 h. Coverslips were washed in PBS three times for 5 min before the addition of Alexa Fluor® conjugated secondary antibodies (Life Technologies) for 1 h. Coverslips were then washed three times for 5 min in PBS, and mounted in ProLong™ Gold Antifade Mountant with DAPI (Life Technologies). All steps were carried out at room temperature. Images were collected using a 40× oil lens with 1.4 NA on a Zeiss LSM 710. Primary antibodies used were LAMP2 (Abl-93, University of Iowa Hybridoma Bank), β-tubulin (5568, Cell Signalling), HA (3F10, Sigma) and MAP2 (ab5392, Abcam).
5
Comprehensive Immunofluorescence Protein Labeling
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TDP-43 (CAC-TIP-TD-P09, Cosmo bio Tokyo, Japan), myc (9E10, Santa Cruz Biotechnology, Dallas, TX, USA), HA (3F10, Sigma), NF-κB p65 (D14E12, Cell Signaling Technology, Danvers, MA, USA), Ran (ab53775, Abcam, Cambridge, UK), RanGAP1 (ab92360, Abcam), Anti-Histone H3 (ab10799, Abcam), LAMP1 (Ly1C6, Enzo life sciences, Farmingdale, NY, USA), TIAR (BD life sciences, Durham, NC, USA), Tumor Necrosis Factor alpha, human recombinant (rHuTNFα, 50435.50, Biomol, Hamburg, Germany), GAPDH (AM4300, ThermoFisher Scientific, Waltham, MA, USA). Anti-GFP (N86/8, UC Davis/NeuroMab Facility, UC Davis, CA, USA), Anti-tRFP antibody (AB233, Evrogen, Russian Federation).
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