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Hpaf 2 cells

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HPAF-II cells are a well-characterized human pancreatic cancer cell line derived from a primary tumor. These cells are adherent and can be used for a variety of in vitro studies.

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Lab products found in correlation

Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Rapamycin, by Cell Signaling Technology (1 mentions) Metformin hydrochloride, by Bio-Techne (1 mentions)

Close spelling variants of this product

HPAF-II (136 citations) CD18/HPAF-II cells (2 citations)

2 protocols using hpaf 2 cells

1

Cell Culture Protocol: MiaPaCa-2, HPAF-II, and HeLa-NF-κB Cells

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MiaPaCa-2 cells were obtained from Dr. Thiruvengadam Arumugam (MD Anderson Cancer Center, Houston, TX, USA) and grown in DMEM. HPAF-II cells were purchased from ATCC (Manassas, VA, USA) and grown in EMEM media. HeLa-NF-κB luciferase cells were purchased from Signosis Inc. (Santa Clara, CA, USA) and were grown in DMEM supplemented with hygromycin B (50 µg/mL). All the cells were maintained in their respective media supplemented with 10% FBS, 2 mM l-glutamine, penicillin (100 U/mL), and streptomycin (10 mg/mL) and maintained at 37 °C in a 5% CO2 humidified incubator.
Muralidharan-Chari V., Kim J., Abuawad A., Naeem M., Cui H, & Mousa S.A. (2016). Thymoquinone Modulates Blood Coagulation in Vitro via Its Effects on Inflammatory and Coagulation Pathways. International Journal of Molecular Sciences, 17(4), 474.
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2

Metformin and Torin 2 Efficacy Study

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Reagents were obtained from the following sources: Metformin hydrochloride (N,N-Dimethyllimidodicarbonimidic diamide hydrochloride) (cat # 2864) and Torin 2 (9-(6-Aminopyridin-3-yl)-1-(3-trifluromethyl)-phenyl)benozo[h][1,6]naphthyridin-2(1H) (Cat # 4248) were obtained from from Tocris Bioscience (R & D Systems, USA). Rapamycin was obtained from Cell Signaling (Cat # 9904). Other chemicals were obtained either from Sigma or Fisher. Immobilon-P polyvinylidene difluoride membrane (0.45 μm) was from Millipore, reagents for enhanced chemiluminescence (ECL) were from Millipore (Immobilon Western chemiluminescent horseradish peroxidase). HPAF II cells (Cat # CRL 1997), EMEM media (Cat 30-2003); Fetal Bovine Serum (Cat # 30-2020) were purchased from ATCC.
Soliman G.A., Steenson S.M, & Etekpo A.H. (2016). Effects of Metformin and a Mammalian Target of Rapamycin (mTOR) ATP-Competitive Inhibitor on Targeted Metabolomics in Pancreatic Cancer Cell Line. Metabolomics : open access, 6(3), 183.
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