All reagents were purchased from Sigma-Aldrich, Life Technologies, or Fisher Chemical and were of analytical grade. Acetonitrile hypergrade for LC-MS LiChrosolvR was supplied by Merck KGaA (Darmstadt, Germany). Methanol hypergrade for LC-MS LiChrosolvR was supplied by Merck KGaA (Darmstadt, Germany). Acetic acid was obtained from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). The Purelab Classic (ELGA LabWater, High Wycombe, Bucks, UK) was used to generate high purity water for preparation of aqueous mobile phase.
Purelab classic
Manufactured by Elga LabWater
Sourced in United Kingdom
The Purelab Classic is a water purification system designed to produce high-quality ultrapure water. It employs a multi-stage purification process to remove impurities and meet the specifications required for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Caffeine, by Merck Group (2 mentions)
Jacobine, by Phytoplan (2 mentions)
Retrorsine n oxide, by Phytoplan (2 mentions)
Jacobine n oxide, by Phytoplan (2 mentions)
Formic acid, by Merck Group (2 mentions)
Intermedine, by Phytoplan (2 mentions)
Retrorsine, by Phytoplan (2 mentions)
Senkirkine, by Phytoplan (2 mentions)
Indicine n oxide, by Phytoplan (2 mentions)
Echimidine, by Phytolab (2 mentions)
Echimidine n oxide, by Phytolab (2 mentions)
Heliotrine, by Phytolab (2 mentions)
Heliotrine n oxide, by Phytolab (2 mentions)
Lycopsamine, by Phytolab (2 mentions)
Lycopsamine n oxide, by Phytolab (2 mentions)
Acetic acid, by Merck Group (1 mentions)
Lichrosolvr, by Merck Group (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
10 protocols using purelab classic
1
Analytical Grade Chemical Procurement
2
Synthesis of Fluorescent Thermoresponsive Hydrogels
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N-isopropylacrylamide, ≥99%, (NIPAM), N,N’-methylenebisacrylamide (BIS), potassium persulfate (KPS), fluorescein isothiocyanate isomer I (FITC), fluorescein O-methacrylate (Fluo-MA) (see Figure S1 of Supporting Material ), poly(ethylene glycol) average Mn 4000 Da, (PEG), dimethyl sulfoxide (DMSO), sodium carbonate and sodium bicarbonate were purchased from Sigma-Aldrich (Merck Life Science S.r.l., Milan, Italy). Methacryloxyethyl thiocarbamoyl rhodamine B (Rhod-MA) (see Figure S2 of Supporting Material ) was purchased from Polysciences (Polysciences Europe GmbH, Hirschberg, Germany). All products were reagent grade and used as received. Water was Milli-Q purity grade (18.2 MΩ cm), produced with a deionization–ultrafiltration apparatus (Elga Pure-Lab Classic, ELGA LabWater, High Wycombe, UK) and filtered prior to use (nylon syringe filter, 0.20 μm pore size).
3
Fabrication of Silk Fibroin Solution
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Silk fibroin solution was prepared as previously reported.31 (link) Briefly, B. mori cocoons were cut into small pieces and rinsed with ultra-purified water with a specific resistivity of 18.2 MΩ cm (PURELAB Classic, ELGA LabWater, LLC). After drying, 2.5 gram cocoons were added to 1 L of boiling 0.02 M sodium carbonate (Na2CO3) (Sigma Aldrich) for degumming 15 min, 30 min, and 60 min, respectively to examine the effect of the degumming time. After degumming, the remaining fibroin bundle was rinsed with ultra-purified water thoroughly and dried overnight. The dried fibroin bundle was then dissolved in a 9.3 M lithium bromide (LiBr) solution with a ratio of 1 to 4. Then we put them in a water bath at 60 °C for at least 4 hours. After being fully dissolved, the solution was then dialyzed against 1 L of ultra-purified water (water was changed after 1, 4, 8, 12, 24, 36, and 40 hours) with a 3–12 mL dialysis cassette (3500 MWCO). The solution was then centrifuged to remove insoluble impurities and the final purified silk fibroin solution was stored at 4 °C for further usage.
4
Sodium Alginate Hydrogel Synthesis
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Sodium alginate (Na-Alg) was purchased from Sigma-Aldrich, Dorset, UK (CAS: 9005-38-3) and dissolved in ultrapure water to ethanol absolute = 8 : 2 solution. Ultrapure water was produced with a Purelab Classic from Elga Labwater, Lane End, High Wycombe, UK. ethanol absolute was purchased from Tianli Chemical Reagent Co., Ltd., Tian Jin City, China. CaCl2 anhydrous was obtained from Heng Xing, Tianjin, China and dissolved in ultrapure water. Polyallylamine hydrochloride (PAH) (MW = 50.000 g mol−1) and fluorescein isothiocyanate (FITC) (MW = 389.38 g mol−1) were purchased from Sigma (St. Louis, USA). FITC was labelled to PAH according to ref. 29 (link) PAH-FITC was used for fluorescence marking and dissolved in ultrapure water at a concentration of 2 g L−1.
5
Cationic Surfactant Comparative Analysis
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ACS reagent acetone and absolute methanol were purchased from Sigma-Aldrich (St Quentin Fallavier, France). Cationic surfactants used for comparative purposes, dodecyltrimethylammonium chloride (DTAC) and tetradecyltrimethylammonium chloride (TTAC), were also the products of Sigma-Aldrich with a high purity (purum, ≥98.0%). ACS reagent 1H-Pyrazol-1-carboxamidine hydrochloride and primary amines, decylamine, dodecylamine, tetradecylamine, and hexadecylamine were obtained from ABCR (Karlsruhe, Germany). All chemicals were employed without further purification. The 18.2 MΩ-cm water utilized in calorimetry, conductivity, and surface tension measurements was obtained with the aid of a combined Purite Select Analyst (France Eau, Fargues St Hilaire, France) and PURELAB® Classic (ELGA LabWater, Atony, France) water purification system.
6
Quantification of Pyrrolizidine Alkaloids
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Analytical standards were obtained from different suppliers as follows: echimidine (purity 97%), echimidine N-oxide (purity 97%), heliotrine (purity 91%), heliotrine N-oxide (purity 91%), lycopsamine (purity 80%) and lycopsamine N-oxide (purity 80%) from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany); senecionine (purity 99%), senecionine N-oxide (purity 99%), seneciphylline (purity 94%) and seneciphylline N-oxide (purity 94%) from Carl Roth & Co. KG (Karlsruhe, Germany); indicine-N-oxide (purity 99%), intermedine (purity 99%), jacobine (purity 98%), jacobine N-oxide (purity 98%), retrorsine (purity 90%), retrorsine N-oxide (purity 96.0%) and senkirkine (purity 98%) from Phytoplan (Heidelberg, Germany); caffeine (purity 98%), used as an internal standard, was from Sigma-Aldrich (Steinheim, Germany).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
7
Analytical Grade Chemicals for LC-MS
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Methanol (MeOH) and acetonitrile (ACN) LC-MS grade and formic acid (FA) purity 99% were obtained from Carlo Erba Reagents (Chaussée du Vexin, France). Petroleum ether and diethyl ether analytical grade were purchased from Carl Roth GmbH (Karlsruhe, Germany), and deuterochloroform (CDCl 3 ) was obtained from Euriso-Top (Saint-Aubin, France). Potassium permanganate was obtained from Acros Organics (Geel, Belgium).
Ultrapure water was provided by a Purelab Classic (ELGA LabWater, High Wycombe, Bucks, UK).
Ultrapure water was provided by a Purelab Classic (ELGA LabWater, High Wycombe, Bucks, UK).
8
Quantification of Pyrrolizidine Alkaloids
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Analytical standards were obtained from different suppliers as follows: echimidine (purity 97%), echimidine N-oxide (purity 97%), heliotrine (purity 91%), heliotrine N-oxide (purity 91%), lycopsamine (purity 80%) and lycopsamine N-oxide (purity 80%) from PhytoLab GmbH & Co. KG (Vestenbergsgreuth, Germany); senecionine (purity 99%), senecionine N-oxide (purity 99%), seneciphylline (purity 94%) and seneciphylline N-oxide (purity 94%) from Carl Roth & Co. KG (Karlsruhe, Germany); indicine-N-oxide (purity 99%), intermedine (purity 99%), jacobine (purity 98%), jacobine N-oxide (purity 98%), retrorsine (purity 90%), retrorsine N-oxide (purity 96.0%) and senkirkine (purity 98%) from Phytoplan (Heidelberg, Germany); caffeine (purity 98%), used as an internal standard, was from Sigma-Aldrich (Steinheim, Germany).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
Methanol (LC-MS grade) and sulphuric acid (98% purity, analytical grade) were from Carlo Erba reagents (Milan, Italy). Ammonia (28% purity, analytical grade) was from VWR Chemicals. Formic acid (98% purity, LC-MS grade) was from Sigma-Aldrich. Ultra-pure water was obtained from a water purification system (Purelab Classic, ELGA Lab Water, High Wycombe, UK).
9
Protein Labeling for Separation Analysis
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Proteins were covalently labeled with a fluorescent dye to ensure they could be readily detected during separations method development studies. β-galactosidase and EGF were labeled by incubating 400 μg of each protein with AZDye 594 1:10 in 100 mM HEPES, pH 8.2 for 1.5 h at room temperature. Excess dye was removed using Amicon ultra centrifugal filters (Millipore Sigma) by washing with 10 mM Tris-HCl and PBS. Protein stock solutions were aliquoted in PBS and stored at -20 °C. All aqueous solutions were prepared using 18.2 MΩ•cm resistance water (Purelab Classic, ELGA LabWater, High Wycombe, UK).
10
PVA Synthesis and Fluorescent Labeling
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Poly(vinyl alcohol) (PVA) with a number-average molecular weight, determined by membrane osmometry, of 30,000 ± 5000 g/mol, and a weight-average molecular weight of 70,000 ± 10,000 g/mol, determined by static light scattering were taken; sodium (meta)periodate (NaIO 4 ), fluorescein isothiocyanate isomer I (FITC), and dimethylsulfoxide (DMSO) were also taken as Sigma-Aldrich (Milan, IT) products. Water with Milli-Q purity grade (18.2 MΩ•cm) was produced using a PureLab Classic (ELGA LabWater, High Wycombe, UK) deionisation/purificaton apparatus.
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