The following antibodies were used for mouse tissues immunohistochemistry and immunofluorescence: Mouse anti-Human TDP-43 (Novus 2E2–D3); Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2); Rabbit anti-Mouse TDP-43 C-Term (Proteintech 12892–1);Rabbit anti-Synaptophysin (Life Technologies Z66, 1:500); Mouse anti-Pan-Axonal neruofilament (Covance, SMI-312R, 1:1000). The following antibodies were used for cortex and spinal cord immunoblot: Mouse anti-Human TDP-43 (Novus 2E2–D3); Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2); Rabbit anti-Mouse TDP-43 C-Term (Proteintech 12892–1); Mouse anti-GAPDH (Sigma, GAPDH-71.1).The following antibodies were used for i3Neuron stable cell lines immunoblot: Rabbit anti-TDP-43 (Proteintech 10782–2-AP 1:1000); Mouse anti-FLAG (Sigma F1804, 1:5000); Rabbit anti-GAPDH (CST 2118, 1:1000); Mouse anti-Tubulin (Thermofisher 14–4502–82, 1:1000). The following antibodies were used for QBI-293 stable cell lines immunoblot: Mouse anti-TDP-43 (abcam ab104223, 1:2000); Rabbit anti-GFP (Cell signalling mAb 2956, 1:1000); Mouse anti-Beta Actin (Proteintech 66009–1, 1:2000). The following antibody was used for HEK-293 cells immunoblot: Rabbit anti-Mouse TDP-43 N-Term (Proteintech 10782–2, 1:2000).
Mouse anti beta actin
Manufactured by Proteintech
Sourced in United States, China
Mouse anti-Beta Actin is a monoclonal antibody that specifically recognizes the beta-actin protein, a highly conserved cytoskeletal protein found in all eukaryotic cells. This antibody can be used for the detection and quantification of beta-actin in various sample types, such as cell lysates and tissue extracts, using techniques like Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Mouse anti gapdh, by Merck Group (1 mentions)
Mouse anti human tdp 43, by Proteintech (1 mentions)
Rabbit anti mouse tdp 43 n term, by Proteintech (1 mentions)
Rabbit anti mouse tdp 43 c term, by Proteintech (1 mentions)
Rabbit anti synaptophysin, by Thermo Fisher Scientific (1 mentions)
Mouse anti human tdp 43, by Novus Biologicals (1 mentions)
Rabbit anti tdp 43, by Proteintech (1 mentions)
Mouse anti tubulin, by Thermo Fisher Scientific (1 mentions)
Mouse anti tdp 43, by Abcam (1 mentions)
Rabbit anti gfp, by Cell Signaling Technology (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Smi 312r, by Fortrea (1 mentions)
Ecl kit, by Proteintech (1 mentions)
Ab126832, by Abcam (1 mentions)
Ab20034, by Abcam (1 mentions)
Ab32364, by Abcam (1 mentions)
Enhanced chemi luminescence (ecl), by Bio-Rad (1 mentions)
Enhanced chemiluminescence, by Beyotime (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
4 protocols using mouse anti beta actin
1
Antibodies for TDP-43 Immunohistochemistry and Immunoblotting
2
Western Blot Analysis of DDX21 and Actin
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Cells were lysed in buffer containing NP-40. Equal amounts of protein were separated by SDS-PAGE. After incubation with the indicated antibodies, the immune complexes on the membrane were detected by an ECL kit (Proteintech, # PK10002). The following antibodies were acquired from commercial sources: mouse anti-DDX21 (Proteintech, #10528-1-AP) and mouse anti-Beta Actin (Proteintech, #66009-1-Ig).
3
Quantification of CD27+/- Treg Cells
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The quantity of CD27+/− Tregs was measured using a BCA Protein Assay
Kit (23227; Thermo Fisher Scientific, Lithuania). The antibodies used in this
study were mouse anti-human Foxp3 (1:1,000, #ab20034; Abcam, UK), rabbit
anti-Helios (1:1,000, #42427T; CST, USA), rabbit anti-STAT5 (1:1,000, #ab126832;
Abcam, UK), rabbit anti-phosphorylated-STAT5 (p-STAT5, 1:1,000, #ab32364; Abcam,
UK), rabbit anti-JAK3 (1:1000, #8827T; CST, USA), and rabbit
anti-phosphorylated-JAK3 (1:1000, #8827S; CST, USA). Mouse anti-beta-actin
(1:5,000, #66009-1-Ig; Proteintech, Rosemount, IL, USA) was used as an internal
control. The blots were visualized using enhanced chemiluminescence (ECL;
BioRad, California, USA). Band intensity was analyzed using Quantity One
software.
Kit (23227; Thermo Fisher Scientific, Lithuania). The antibodies used in this
study were mouse anti-human Foxp3 (1:1,000, #ab20034; Abcam, UK), rabbit
anti-Helios (1:1,000, #42427T; CST, USA), rabbit anti-STAT5 (1:1,000, #ab126832;
Abcam, UK), rabbit anti-phosphorylated-STAT5 (p-STAT5, 1:1,000, #ab32364; Abcam,
UK), rabbit anti-JAK3 (1:1000, #8827T; CST, USA), and rabbit
anti-phosphorylated-JAK3 (1:1000, #8827S; CST, USA). Mouse anti-beta-actin
(1:5,000, #66009-1-Ig; Proteintech, Rosemount, IL, USA) was used as an internal
control. The blots were visualized using enhanced chemiluminescence (ECL;
BioRad, California, USA). Band intensity was analyzed using Quantity One
software.
4
Western Blot Analysis of SNAP-25 in Spinal Cord
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Spinal cord tissues were harvested as described previously. Tissues were homogenized in protein extraction reagent (Beyotime Biotechnology, Nantong, China). In detail, proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA) in transfer buffer. Membranes were blocked by 5% skim milk and incubated with monoclonal rabbit anti-SNAP-25 (1:200; Boster, Wuhan, Hebei Province, China) or mouse anti-beta-actin (1:4,000; ProteinTech, Chicago, IL, USA) overnight at 4°C. Horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG (1:10,000; ProteinTech) antibodies were applied for 2 hours at 37°C after membranes were washed by Tris-buffered saline containing 0.5% Tween-20. Membranes were detected by Enhanced Chemiluminescence (Beyotime Biotechnology) and exposure to film in the dark. The optical density intensity of each band was measured using Quantity One software (BioRad, Waltham, MA, USA). Results were shown as the optical density ratio to beta-actin.
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