A1r live cell confocal

Manufactured by Nikon

The Nikon A1R Live Cell Confocal is a high-performance confocal microscope designed for live-cell imaging. It features a resonant scanner for rapid image acquisition and a sensitive detector for low-light applications. The A1R Live Cell Confocal provides optical sectioning capabilities to enable the visualization of 3D cellular structures within living specimens.

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Lab products found in correlation

Trypan blue, by Merck Group (1 mentions)

Close spelling variants of this product

A1R-TiE live-cell imaging confocal system Nikon A1R Live Cell Confocal microscope A1R Live Cell Imaging Confocal Microscope A1R Live Cell Inverted Confocal microscope A1R confocal

2 protocols using a1r live cell confocal

Imaging Membrane-Associated LPS in LSECs

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LSEC isolated from C57BL/6 mice liver was seeded to collagen-coated dishes for 2h in RMPI media containing 10% FBS and then washed twice. The LPS or LPS with different concentrations of HDL plus LBP was then incubated with LSEC cells for different time points and washed twice with 4°C RPMI media without FBS. Cells were analyzed using Nikon A1R Live Cell Confocal for imaging with plan Apo optics of 60x in oil, laser wavelength at 487.8nm, laser power 1.0, pinhole radius at 28.1μm, calibration of 0.21μm/px. The membrane-associated LPS was then quenched with 4mg/ml Trypan blue (Sigma) in PBS. Fluorescent images after quenching of Trypan blue were obtained at the same spot. The images were quantified by ImageJ.

Cabral F., Al-Rahem M., Skaggs J., Thomas T.A., Kumar N., Wu Q., Fadda P., Yu L., Robinson J.M., Kim J., Pandey E., Sun X., Jarjour W.N., Rajaram M.V., Harris E.N, & Ganesan L.P. (2021). Stabilin receptors clear LPS and control systemic inflammation. iScience, 24(11), 103337.

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Fluorescent Peptide Uptake in HeLa Cells

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HeLa cells (5 × 10⁴ cells/mL) were seeded in a 35-mm glass-bottomed microwell dish (Grenier Bio-One) and cultured overnight. The next day, cells were treated with 5 μM fluorescein-labeled peptide 1 or 2 for 2 h in DMEM media containing 1% FBS and 1% Abs. After removal of the media, the cells were gently washed with DPBS twice and imaged on a Nikon A1R live-cell confocal equipped with a 100X oil objective. Data was analyzed using NIS-Elements AR.

Salim H., Song J., Sahni A, & Pei D. (2019). Development of a Cell-Permeable Cyclic Peptidyl Inhibitor against the Keap1-Nrf2 Interaction. The Journal of organic chemistry, 85(3), 1416-1424.

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