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Epr 800

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The EPR-800 is a compact and versatile electron paramagnetic resonance (EPR) spectrometer designed for a wide range of applications. It offers high-performance capabilities and is suitable for various research and analytical tasks.

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Lab products found in correlation

Stg 3008, by MultiSciences Biotech (4 mentions) Powerlab, by ADInstruments (2 mentions) Labchart, by ADInstruments (1 mentions) Bio amp, by ADInstruments (1 mentions) Labchart software, by ADInstruments (1 mentions) Ecgauto2, by EMKA Technologies (1 mentions) Norepinephrine bitartrate, by Merck Group (1 mentions) Powerlab station, by ADInstruments (1 mentions)

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Millar 1.1F octapolar EP catheter (EPR-800; (2 citations)

21 protocols using epr 800

1

Cardiac Electrophysiology Protocols in Mice

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Simultaneous atrial and ventricular pacing and recording were performed via 1.1F octapolar catheter (EPR-800, Millar) inserted via a jugular vein in mice anesthetized with isoflurane (2–3%). The standard pacing protocols11 (link)–13 (link) were programmed in the multichannel stimulator STG 3008 (Multichannel systems), which was analyzed by LabChart (ADInstruments). Sinus nodal function was evaluated by measuring sinus node recovery time (SNRT) at three pacing drive rates (150, 120 and 100 ms) and corrected SNRT (SNRT minus sinus cycle length, SCL). AV conduction properties were assessed with rapid atrial pacing at rates with a minimum pacing cycle length of 50 ms (up to 1,200 bpm). The minimum cycle length maintaining 1:1 AV conduction, the Wenckebach paced cycle length, and the maximum paced cycle length causing 2:1 AV block were determined. AV nodal effective refractory periods were determined by the maximum coupling interval causing AV block at the two pacing drive rates (150 and 120 ms). Right ventricular effective refractory periods were determined by the maximum coupling interval that failed to stimulate ventricles at the two pacing drive rates (150 and 120 ms). Right ventricular burst pacing was performed at rates with a pacing cycle length of 150 to 50 ms (400-1,200 bpm) to assess retrograde ventriculo-atrial conduction.
Chowdhury R., Ashraf H., Melanson M., Tanada Y., Nguyen M., Silberbach G.M., Wakimoto H., Benson D.W., Anderson R.H, & Kasahara H. (2015). A Mouse Model of Human Congenital Heart Disease: Progressive Atrioventricular Block Induced by a Heterozygous Nkx2-5 Homeodomain Missense Mutation. Circulation. Arrhythmia and electrophysiology, 8(5), 1255-1264.
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Ventricular Arrhythmia Induction Protocol

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An octapolar catheter (EPR-800, Millar) was inserted into the right jugular vein and positioned in the right atrium and ventricle. Ventricular effective refractory periods were measured using electrical stimulation with overdrive pacing trains at 100 ms, followed by single extra-stimuli. Ventricular arrhythmia induction was performed with triple extra-stimuli and pacing at gradually faster rates to a pacing cycle length of 10 ms.
Grune J., Lewis A.J., Yamazoe M., Hulsmans M., Rohde D., Xiao L., Zhang S., Ott C., Calcagno D.M., Zhou Y., Timm K., Shanmuganathan M., Pulous F.E., Schloss M.J., Foy B.H., Capen D., Vinegoni C., Wojtkiewicz G.R., Iwamoto Y., Grune T., Brown D., Higgins J., Ferreira V.M., Herring N., Channon K.M., Neubauer S., Sosnovik D.E., Milan D.J., Swirski F.K., King K.R., Aguirre A.D., Ellinor P.T, & Nahrendorf M. (2022). Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nature cardiovascular research, 1(7), 649-664.
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Ventricular Arrhythmia Induction Protocol

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An octapolar catheter (EPR-800, Millar) was inserted into the right jugular vein and positioned in the right atrium and ventricle. Ventricular effective refractory periods were measured using electrical stimulation with overdrive pacing trains at 100 ms, followed by single extra-stimuli. Ventricular arrhythmia induction was performed with triple extra-stimuli and pacing at gradually faster rates to a pacing cycle length of 10 ms.
Grune J., Lewis A.J., Yamazoe M., Hulsmans M., Rohde D., Xiao L., Zhang S., Ott C., Calcagno D.M., Zhou Y., Timm K., Shanmuganathan M., Pulous F.E., Schloss M.J., Foy B.H., Capen D., Vinegoni C., Wojtkiewicz G.R., Iwamoto Y., Grune T., Brown D., Higgins J., Ferreira V.M., Herring N., Channon K.M., Neubauer S., Sosnovik D.E., Milan D.J., Swirski F.K., King K.R., Aguirre A.D., Ellinor P.T, & Nahrendorf M. (2022). Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nature cardiovascular research, 1(7), 649-664.
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In Vivo Electrophysiology Study in Mice

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An in vivo EPS was performed 2–4 weeks after PAC or sham operation. Mice were anaesthetised with a mixture of 0.3 mg/kg medetomidine, 4.0 mg/kg midazolam, and 5.0 mg/kg butorphanol. The heart rate was maintained at 400–500 bpm during the EPS. Programmed electrical stimulations (burst pacing and extrastimulus pacing) were delivered from the right ventricle using 1.1-Fr electrophysiology catheters (EPR-800, Millar, Houston, TX, USA), and electrocardiograms were recorded by electrodes placed on the extremities.
Ito S., Hashimoto H., Yamakawa H., Kusumoto D., Akiba Y., Nakamura T., Momoi M., Komuro J., Katsuki T., Kimura M., Kishino Y., Kashimura S., Kunitomi A., Lachmann M., Shimojima M., Yozu G., Motoda C., Seki T., Yamamoto T., Shinya Y., Hiraide T., Kataoka M., Kawakami T., Suzuki K., Ito K., Yada H., Abe M., Osaka M., Tsuru H., Yoshida M., Sakimura K., Fukumoto Y., Yuzaki M., Fukuda K, & Yuasa S. (2022). The complement C3-complement factor D-C3a receptor signalling axis regulates cardiac remodelling in right ventricular failure. Nature Communications, 13, 5409.
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Cardiac Electrophysiology Stimulation Protocols

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A series of in vivo experiments applied programmed stimulation procedures. This used an ultraminiature octapolar 1.1F electrophysiological catheter (EPR-800, Millar Instrument, Inc., Houston, TX, USA) inserted via the jugular vein and placed into the right atrium. The stimulation protocols were performed after 10 min of stable simultaneous recordings of the baseline surface ECG.
Pacing protocols including (i) sinus node recovery time (SNRT): deliver atrial pace at drive cycle lengths of 100 ms for 30 s. Three runs with 10 s intervals; (ii) atrioventricular (AV) node conduction: deliver atrial burst pacing starting at 150 ms and decremented by 5 ms every train and down to AV Wenckebach cycle length. Continue delivering atrial burst pacing and decrement by 5 ms down to 2 : 1 conduction; and (iii) atrial fibrillation threshold (AFT): three trains of 20 pulses at 20 ms cycle length, with 2 s interval between trains, starting from twice threshold and incrementally increased by 1 mA until induction of AF.
Jung E., Capel R., Jiang C., Venturi E., Neagu G., Pearcey S., Zhou Y., Zhang Y, & Lei M. (2023). Cardiac deficiency of P21-activated kinase 1 promotes atrial arrhythmogenesis in mice following adrenergic challenge. Philosophical Transactions of the Royal Society B: Biological Sciences, 378(1879), 20220168.
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Intracardiac Electrophysiology in Mouse Model

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Intracardiac electrophysiological recording and stimulation was performed on mice sedated with inhaled isofluorane. A 1.1-F octapolar catheter (Millar EPR-800) was inserted through the right jugular vein and into the right atria and ventricle in mice sedated with inhaled isoflurane. Animals were positioned prone on a heated table and surface electrocardiograms were measured with limb leads in the table. Protocols for burst pacing of 50 ms cycle length were used to try and elicit bouts of atrial tachycardia or atrial fibrillation [28 (link)]. If atrial tachycardia was observed in two or more burst pacing protocols the animal was considered positive. Following baseline recordings, animals were treated with intraperitoneal injection of 4 mg/kg isoproterenol and burst pacing was repeated to assess the occurrence of arrhythmia.
Barefield D.Y., Yamakawa S., Tahtah I., Sell J.J., Broman M., Laforest B., Harris S., Alvarez A.A., Araujo K.N., Puckelwartz M.J., Wasserstrom J.A., Fishman G.I, & McNally E.M. (2022). Partial and complete loss of myosin binding protein H-Like cause cardiac conduction defects. Journal of molecular and cellular cardiology, 169, 28-40.
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Transesophageal Burst Pacing for Atrial Fibrillation Induction

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Transesophageal burst pacing was performed after 6 weeks of SD intervention. Mice underwent anesthesia with 1.5–2% isoflurane, and electrodes were fixed on limbs to yield a surface II-lead echocardiogram (ECG). AF was induced via transesophageal burst rapid pacing with a 1.1-Fr octapolar catheter (EPR-800, Millar Instruments, Houston, TX, USA) [32 (link)]. Each mouse was stimulated five times continuously. AF was defined as an abnormal ECG with an irregular atrial rhythm, P wave loss, and irregular R-R intervals, persisting for at least 1 s. Successful AF induction was defined as at least 2/5 episodes of AF. In addition, the percentage of mice with induced AF was displayed as AF inducibility, which was verified by an experienced cardiac electrophysiologist.
Zuo K., Fang C., Fu Y., Liu Z., Liu Y., Liu L., Wang Y., Wang H., Yin X., Liu X., Li J., Zhong J., Chen M., Yang X, & Xu L. (2022). The Impact of Sleep Disturbance on Gut Microbiota, Atrial Substrate, and Atrial Fibrillation Inducibility in Mice: A Multi-Omics Analysis. Metabolites, 12(11), 1144.
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8

Intracardiac Electrogram Acquisition in Mice

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Detailed protocols for intracardiac electrograms have been previously described (Nadadur et al., 2016 (link)). Briefly, 8- to 10- week-old mice were anesthetized with isoflurane and a vertical skin cut-down at the right jugular vein was performed. A 1.1 F octapolar catheter (EPR-800, Millar Instruments) was advanced in the right jugular vein to perform electrical stimulation. The catheter was connected to ADI BioAmp and PowerLab apparatus and signals were recorded using LabChart Software (ADInstruments). Atrial induction pacing was performed using burst pacing and the presence of at least three cycles of atrial tachycardia or fibrillation at least twice was considered positive.
Dai W., Laforest B., Tyan L., Shen K.M., Nadadur R.D., Alvarado F.J., Mazurek S.R., Lazarevic S., Gadek M., Wang Y., Li Y., Valdivia H.H., Shen L., Broman M.T., Moskowitz I.P, & Weber C.R. (2019). A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. eLife, 8, e41814.
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9

Induction of Atrial Arrhythmias in Mice

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Programmed intracardiac stimulation was performed to evaluate inducibility of atrial arrhythmias as previously described (68 (link)). Briefly, an incision was made to the right of the midline near the clavicle to access the right jugular vein for insertion of a 1.1F octapolar catheter (EPR-800; Millar Instruments) into the right atrium and ventricle. Atrial arrhythmias were induced using a burst pacing protocol where a 2-second burst with a cycle length of 40 ms was applied followed by successive bursts each with 2 ms decrements down to a cycle length of 10 ms. Pacing protocols were performed in triplicate and mice were considered positive for arrhythmia inducibility if pacing evoked arrhythmias lasting 1 second or longer, at least 2 out of 3 times. The incidence of inducible atrial arrhythmia was calculated as the percentage of arrhythmia-positive mice divided by the total number of mice studied.
Rao A.N., Campbell H.M., Guan X., Word T.A., Wehrens X.H., Xia Z, & Cooper T.A. (2021). Reversible cardiac disease features in an inducible CUG repeat RNA–expressing mouse model of myotonic dystrophy. JCI Insight, 6(5), e143465.
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10

Intracardiac Electrophysiology in Mice

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Mice were anesthetized with isoflurane and placed on a heated pad to maintain body temperature at 37°C. A 1.1 French octapolar electrode catheter (EPR-800, Millar Instruments, Houston, TX) was advanced through the right jugular vein into the right atrium and right ventricle. Programmed electrical stimulation was performed using 2-ms current pulses delivered by an external stimulator (STG3008, Multichannel Systems, Reutlingen, Germany). Surface 6-lead ECG was recorded and monitored throughout the experimental procedures. The 8-lead intracardiac electrograms were recorded and analyzed with a computer-based data acquisition system (IOX-2 acquisition software and ecgAUTO2 software, Emka Technologies, VA, USA).
Right atrial overdrive pacing protocols of either S1S2 or S1S1 stimulus were used to determine atrial effective refractory period (AERP),, Wenckebach point, 2:1 atrioventricular block point or atrial tachycardia, respectively. Right ventricular overdrive pacing protocols of either S1S2 or S1S1 stimulus were performed to determine ventricular effective refractory period (VERP) or inducibility of ventricular tachycardia, respectively..
Yu Y., Ye L., Li Y.G., Burkin D.J, & Duan D.D. (2016). Heart-Specific Overexpression of the Human Short CLC-3 Chloride Channel Isoform Limits Myocardial Ischemia-induced ERP and QT Prolongation. International journal of cardiology, 214, 218-224.
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