Baicalin

Manufactured by Chengdu Must Bio-Technology

Sourced in China

Baicalin is a naturally-occurring compound extracted from the roots of Scutellaria baicalensis, a medicinal plant. It serves as a key active ingredient in various laboratory procedures and research applications. The core function of Baicalin is to act as a potent antioxidant and anti-inflammatory agent, with potential applications in areas such as cell culture studies and biochemical assays. Further details on intended use would require a more specialized discussion.

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Lab products found in correlation

8 protocols using baicalin

Isolation and Identification of Phytochemicals from Forsythia suspensa

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Lianqiaoxinside A was isolated by the author from the fruits of F. suspensa. Chlorogenicacid, caffeic acid, forsythiaside B, rutin, hyperoside, forsythiaside A, cynaroside, scutellarin, (+)-pinoresinol-β-d-glucoside, isochlorogenic acid A, baicalein, baicalin, phillyrin, phillygenin, arctiin, quercetin, luteolin (≥98.0%)were purchased from the Chengdu MUST Biotechnology Co., Ltd. (Chengdu, China). HPLC grade acetonitrile (ACN) and formic acid were obtained from Fisher Scientific (Waltham, MA, USA). Purified water was used from a Milli-Q system (Millipore, Bedford, MA, USA). All other reagents were of analytical grade.

Liang J., Sun H.M, & Wang T.L. (2017). Simultaneous Determination of Multiple Classes of Hydrophilic and Lipophilic Components in Shuang-Huang-Lian Oral Liquid Formulations by UPLC-Triple Quadrupole Linear Ion Trap Mass Spectrometry. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(12), 2057.

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Baicalin Preparation for Experiments

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Baicalin (purity, ≥98%), a yellowish crystalline powder, was purchased from Chengdu Must Bio-technology Co., Ltd. (Chengdu, China). Prior to experiments, Baicalin was dissolved in 2 ml physiological saline at an initial concentration of 22.4 µmol/ml and stored at −20°C in the dark prior its to use in subsequent experiments.

Huang X., Wu H., Wang L., Zheng L, & Zhao J. (2017). Protective effects of baicalin on rabbit articular chondrocytes in vitro. Experimental and Therapeutic Medicine, 13(4), 1267-1274.

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Baicalin, Wogonoside, and Baicalein Effects

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Baicalin, wogonoside and baicalein (≥98% purity, HPLC) were obtained from Chengdu MUST Bio-technology Company Ltd. (Chengdu, China). SB with the place of origin in Ji Lin province of China was purchased from Beijing Tong Ren Tang Zhuhai Pharmacy Co., Ltd. (Guangdong, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) and ThT were purchased from Sigma (St. Louis, MO, United States). Milli-Q water was prepared by the Milli-Q integral water purification system (Millipore, Billerica, MA, United States) in our laboratory. Acetonitrile was purchased from Anaqua Chemicals Supply (Houston, TX, United States). Aβ (1–42) was obtained from the China Peptides Co., Ltd. (Shanghai, China). EZ-Link NHS-LC-LC-Biotin was obtained from Thermo Scientific Waltham (MA, United States). Super Streptavindin (SSA) biosensors were purchased from FortéBIO, PALL Life Sciences (Port Washington, NY, United States). The Annexin V staining kit was purchased from BD Biosciences (San Jose, CA, United States). The native PAGE Bis-Tris Gel (4–16%), running buffer (20X) and the native PAGE sample buffer were obtained from Invitrogen (Carlsbad, CA, United States). The PVDF membrane was obtained from PALL Life Sciences (Port Washington, NY, United States).

Yu L., Wu A.G., Wong V.K., Qu L.Q., Zhang N., Qin D.L., Zeng W., Tang B., Wang H.M., Wang Q, & Law B.Y. (2019). The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on β-Amyloid Fibrillation From Scutellaria baicalensis. Frontiers in Pharmacology, 10, 194.

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Baicalin Modulates NF-κB Signaling

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Baicalin (purity ≥ 98%) was purchased from Chengdu Must Biotechnology Co., Ltd. (Chengdu, China). Luria-Bertani (LB) medium was obtained from Sigma-Aldrich (St.Louis, MO, USA). Fetal bovine serum (FBS) and DMEM were obtained from Gibco (Invitrogen S.r.l., Milan, Italy). TRIZOL reagent and PrimeScriptTM RT Reagent Kit with gDNA Eraser were purchased from TaKaRa (Da Lian, Liaoning, China). All other chemicals were of reagent grade. Chicken mAb phospho-NF-κB p65 antibodies and chicken mAb Phospho-IκBα were purchased from Sangon Biotech Company (Shanghai, China).

Peng L.Y., Yuan M., Wu Z.M., Song K., Zhang C.L., An Q., Xia F., Yu J.L., Yi P.F., Fu B.D, & Shen H.Q. (2019). Anti-bacterial activity of baicalin against APEC through inhibition of quorum sensing and inflammatory responses. Scientific Reports, 9, 4063.

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Alcohol Precipitation Sampling Protocol

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Alcohol precipitation samples were collected from one sampling point of the enterprise production site of Yangtze River Pharmaceutical Group (Taizhou, China). Sampling was started after the 1.5th hour of the alcohol precipitation process and sampled at intervals of 10 min. In a total of 6 batches, 50 samples were obtained. To ensure the representativeness of the sample set and expand the sample size, 51 additional samples were collected randomly during the whole process.
Standard geniposide (≥99%) and baicalin (≥98%) were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). HPLC-grade methanol, acetonitrile, and phosphoric acid were obtained from Merck (Darmstadt, Germany). Analytical grade anhydrous sodium dihydrogen phosphate was used for the mobile phase. Deionised water was purified using a Milli-Q purification system (Millipore, Bedford, MA, USA).

Ma H., Chen M., Zhang S., Pan H., Chen Y, & Wu Y. (2022). Rapid Determination of Geniposide and Baicalin in Lanqin Oral Solution by Near-Infrared Spectroscopy with Chemometric Algorithms during Alcohol Precipitation. Molecules, 28(1), 4.

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Comprehensive Compound Sourcing for Research

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Ginsenoside Ro (98%), oleanolic acid (99%), Stigmasterol (95%), palmatine hydrochloride (98%), betherine (98%), epiberberine (98%), coptisine (98%), astragalin (98%), isoquercitrin (98%), baicalin (98%), wogonin (98%), chrysophanol (98%), physcion (98%) and Geniposide (98%) were sourced from the Chengdu Must Bio-technology Co., Ltd (Chengdu, Sichuan Province, China). Betaine (98%), nonanedioic acid (99.5%), succinic acid (90%), allantoin (98.5%), rutin (95%), β-sitosterol (95%) and 5-hydroxymethyl furaldehyde (99%) were sourced from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). β-ecdysterone was sourced from J&K Scientific Ltd (Beijing, China).

Liu Y., Yang F., Pu H., Su J., Liu Z., Dhiloo K.H, & Wang Z. (2016). The Sublethal Effects of β-Ecdysterone, a Highly Active Compound from Achyranthes bidentata Blume, on Grape Phylloxera, Daktulosphaira vitifoliae Fitch. PLoS ONE, 11(11), e0165860.

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Quantification of Bioactive Compounds in Lanqin Oral Liquid

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Lanqin oral liquid (lot no. 19122821) was purchased from Yangtze River Pharmaceutical Group (Jiangsu, China). Reference compounds (purity > 98%) of chlorogenic acid, caffeic acid, scutellarin, baicalin, palmatine, wogonoside, apigenin, baicalein, wogonin, indirubin, and indigo were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). Acetonitrile and formic acid of HPLC grade were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Purified water was purchased from Hangzhou Wahaha Corporation (Hangzhou, China). All other chemical reagents used in this study were of analytical grade.

Liu T, & Lin S. (2023). Systematic Screening of the Chemical Constituents of Lanqin Oral Liquid by Ultra-High-Performance Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Molecules, 28(20), 7053.

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Quantitative Analysis of Bioactive Compounds

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LOS samples of 101 production batches were provided by Yangtze River Pharmaceutical Group (Taizhou, China). The relationship between the production batch number and self-numbering of the sample is shown in Table S1.
Standards of epigoitrin (purity > 99.0%, catalog no: A0529), geniposide (purity > 99.0%, catalog no: A0178) and baicalin (purity > 98.0%, catalog no: A0016) were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). HPLC-grade methanol, acetonitrile and phosphoric acid were obtained from Merck (Darmstadt, Germany). Deionized water was purified by a Milli-Q purification system (Millipore, Bedford, MA, USA).
The murine macrophage RAW 264.7 cell line and ZQ-120 Dulbecco’s modified Eagle medium (DMEM) were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China). NO assay kits (S0021) were provided by Beyotime (Nanjing, China). The phosphate-buffered solution was obtained from Labgic Technology Co., Ltd. (Hefei, China). LPS was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA).

Ma H., Xiao L., Xu D., Geng Y., Liu X., Chen Y, & Wu Y. (2022). Non-Invasive Detection of Anti-Inflammatory Bioactivity and Key Chemical Indicators of the Commercial Lanqin Oral Solution by Near Infrared Spectroscopy. Molecules, 27(9), 2955.

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