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Mda mb 231

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MDA-MB-231 is a cell line derived from a human breast adenocarcinoma. It is a widely used model in cancer research to study various aspects of breast cancer biology.

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Lab products found in correlation

Mda mb 231, by American Type Culture Collection (33 mentions) Dulbecco modified eagle medium (dmem), by Corning (24 mentions) Mcf 7, by Corning (23 mentions) Mcf 7, by American Type Culture Collection (17 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions) Rpmi 1640, by Corning (10 mentions) Mda mb 468, by Corning (9 mentions) Mcf 10a, by American Type Culture Collection (9 mentions) Rpmi 1640 medium, by Corning (8 mentions) Mda mb 468, by American Type Culture Collection (7 mentions) Penicillin streptomycin, by Corning (7 mentions) Roswell park memorial institute (rpmi), by Corning (7 mentions) Fetal bovine serum (fbs), by Corning (6 mentions) Hydrocortisone, by Merck Group (6 mentions) Hs578t, by American Type Culture Collection (5 mentions) Skbr3, by American Type Culture Collection (5 mentions) Bt549, by American Type Culture Collection (5 mentions) Mcf 10a, by Thermo Fisher Scientific (5 mentions) Insulin, by Merck Group (5 mentions) Streptomycin, by Corning (4 mentions)

Close spelling variants of this product

MDA MB 231 cells (2 citations) Human MDA-MB-231 (2 citations)

61 protocols using mda mb 231

1

Cancer Cell Line Culture Protocols

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Cancer cell lines were purchased from the American Tissue Culture Collection (ATCC) or Thermo Fisher Scientific. MDA-MB-231, BT-549, MCF7, DLD-1, and 4T1-luc cells were cultured in RPMI 1640 (Corning), HeLa, HEK293T, and HEK293FT cells in DMEM (Corning), MDA-MB-231-luc cells in MEM (Corning), and HCT116 cells in McCoy’s 5A (Corning). The above media were supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, and 100 U/ml penicillin and streptomycin. All in vitro experiments were performed using multiple-well plates or dishes pre-treated with Matrigel (BD no. 356234) except tumorsphere assays and ELDA that used ultra-low attachment plates and migration/invasion assays that used commercial 24-well plates with inserts. Glioblastoma stem cells (GSCs) were cultured in Neurobasal medium (Thermo Fisher Scientific no. 11360070) supplemented with B27 without vitamin A (Thermo Fisher Scientific no. A3353501), EGF, and bFGF (20 ng/ml each, R&D Systems), sodium pyruvate (Thermo Fisher Scientific no. 11360070), and GlutaMAX (Thermo Fisher Scientific no. 35050061). All cells were incubated at 37°C in 5% CO2. Cell cultures were authenticated by short tandem repeat (STR) analysis.
Shen J.Z., Qiu Z., Wu Q., Zhang G., Harris R., Sun D., Rantala J., Barshop W.D., Zhao L., Lv D., Won K.A., Wohlschlegel J., Sangfelt O., Laman H., Rich J.N, & Spruck C. (2022). A FBXO7/EYA2-SCFFBXW7 Axis Promotes AXL-mediated Maintenance of Mesenchymal and Immune Evasion Phenotypes of Cancer Cells. Molecular cell, 82(6), 1123-1139.e8.
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2

Culturing Human Breast Cancer Cell Lines

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Hs578T, MDA-MB-231, MDA-MB-436, MDA-MB-468, and HCC1806 human breast cancer cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). MDA-MB-231 LM1 was kindly provided by Professor SJ Lee (Hanyang University, Seoul, Korea). Hs578T, MDA-MB-231, MDA-MB-436, MDA-MB-468, and MDA-MB-231 LM1 were maintained in DMEM (Corning, NY, USA) containing 10% fetal bovine serum (Corning, NY, USA) and 1% antibiotic-antimycotic (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). HCC1806 was maintained in RPMI 1640 (Corning, NY, USA) containing 10% fetal bovine serum (Corning, NY, USA) and 1% antibiotic-antimycotic (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) All cell lines were incubated at 37 °C in 5% CO2 in a humidified atmosphere.
Son S., Kim H., Lim H., Lee J.H., Lee K.M, & Shin I. (2023). CCN3/NOV promotes metastasis and tumor progression via GPNMB-induced EGFR activation in triple-negative breast cancer. Cell Death & Disease, 14(2), 81.
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3

Breast Cancer Cell and TAM Co-culture

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Human MDA-MB-231, T47D, MCF7, SKBR3 and THP-1 cell lines were all obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and passed the test of DNA profiling (short tandem repeat (STR) profiling method). All these breast cancer (BC) cells (MDA-MB-231, T47D, MCF7 and SKBR3) were cultured following the instructions of the American Type Culture Collection (ATCC, Manassas, VA, USA). For M2 tumor-associated macrophages (M2-TAMs) generation, as previously described, THP-1 cells were first treated with 200 nM Phorbol-12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) for 24 h, then PMA-THP-1 cells (polarized into macrophages) were cultured by the addition of conditioned media from BC cells (MDA-MB-231, T47D, MCF7 and SKBR3) for another 48 h. In a co-culture system, THP-1 cells were differentiated in upper chamber (0.4 μm pore) (Corning, USA), and BC cells (MDA-MB-231, T47D, MCF7 and SKBR3) were seeded in the lower chamber. After 48 h, TAMs or BC cells were harvested for further study.
He Y., Zhou S., Deng F., Zhao S., Chen W., Wang D., Chen X., Hou J., Zhang J., Zhang W., Ding L., Tang J, & Zhou Z. (2019). Clinical and transcriptional signatures of human CD204 reveal an applicable marker for the protumor phenotype of tumor-associated macrophages in breast cancer. Aging (Albany NY), 11(23), 10883-10901.
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4

Breast Cancer Cell Lines Treated with AnAc 24:1n5

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MCF-7 and MDA-MB-231 cells were purchased from American Type Tissue Collection (ATCC, Manassas, VA). Cells were used at less than 9 passages from ATCC. MCF-7 and MDA-MB-231 cells were maintained in IMEM (Cellgro, Manassas, VA) containing 5% fetal bovine serum (FBS, Atlanta Biologicals, Lawrenceville, GA) and 1% Penicillin/Streptomycin (Cellgro). Cells were grown in phenol red-free IMEM (ThermoFisher) medium containing 5% dextran coated charcoal (DCC)-stripped FBS (hormone-depleted medium) for 48 h prior to treatment with established IC50 concentrations of AnAc 24:1n5: 13.5 μM for MCF-7 and 35.0 μM for MDA-MB-231 cells [13 (link)] for 6 h and was replicated in three separate experiments.
Schultz D.J., Muluhngwi P., Alizadeh-Rad N., Green M.A., Rouchka E.C., Waigel S.J, & Klinge C.M. (2017). Genome-wide miRNA response to anacardic acid in breast cancer cells. PLoS ONE, 12(9), e0184471.
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5

Culturing Human Cancer Cell Lines

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Human cancer cell lines MDA-MB-231, T-47D, 293T, and PC-3 cells were obtained from American Type Culture Collection (ATCC, USA). MDA-MB-231, MCF-7, and 293T cells were maintained in DMEM (Cellgro), and T-47D and PC-3 cells were cultivated in RPMI-1640, medium (Cellgro), all supplemented with 10% FBS (Biowest), penicillin, streptomycin and amphotericin B (Cellgro). Cells were maintained at 37 °C in an incubator with humidified atmosphere, containing 5% CO2.
Jain A., Fournier P.G., Mendoza-Lavaniegos V., Sengar P., Guerra-Olvera F.M., Iñiguez E., Kretzschmar T.G., Hirata G.A, & Juárez P. (2018). Functionalized rare earth-doped nanoparticles for breast cancer nanodiagnostic using fluorescence and CT imaging. Journal of Nanobiotechnology, 16, 26.
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6

Cell Culture Protocols for Breast Cancer Cell Lines

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MDA-MB-231, Hs578t, MCF-7, and MDA-MB-468 cells were purchased from American Type Culture Collection (ATCC). MDA-MB-231 and MCF-7 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Corning CellGro., Manassas, VA, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), 1% pen–strep, and 1% L-glutamine (Corning, CellGro). MDA-MB-468 was cultured in DMEM (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 1% sodium pyruvate, and 1% Minimum Essential Medium (MEM) amino acids (Corning CellGro). Hs578t was cultured in MEM supplemented with 10% fetal bovine serum, 1% pen–strep, and 1% L-glutamine (Corning CellGro). SUM159PT cells were obtained from Asterand and cultured in Ham’s/F-12 (Corning CellGro) supplemented with 10% fetal bovine serum, 1% pen–strep, 1% L-glutamine, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 μg/mL hydrocortisone, and 5 μg/mL insulin. All cell lines were maintained in a humidified incubator at 5% CO2 and 37 °C.
Andrade D., Mehta M., Griffith J., Oh S., Corbin J., Babu A., De S., Chen A., Zhao Y.D., Husain S., Roy S., Xu L., Aube J., Janknecht R., Gorospe M., Herman T., Ramesh R, & Munshi A. (2019). HuR Reduces Radiation-Induced DNA Damage by Enhancing Expression of ARID1A. Cancers, 11(12), 2014.
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7

Cell Culture Conditions for Cancer Research

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Human breast cancer cells (MDA-MB-231), mouse skeletal muscle cells (C2C12), human lung cancer cells (A549), and human hepatocellular carcinoma cells (HepG2) were obtained from the American Type Culture Collection (Rockville, MD, USA). MDA-MB-231 and A549 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Cellgro, Manassas, VA, USA), while C2C12 and HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Cellgro, Lincoln, NE, USA). All culture media were supplemented with 1% penicillin/streptomycin (Gibco BRL, Carlsbad, MD, USA) and 10% fetal bovine serum (Gibco BRL, Carlsbad, MD, USA). The cells were maintained in a 37 °C incubator with a humidified atmosphere containing 5% CO2 and 95% air.
Lee D., Lee S., Jang Y.S., Ryoo R., Kim J.K., Kang K.S, & Kim K.H. (2023). N,N-Dimethyl-anthranilic Acid from Calvatia nipponica Mushroom Fruiting Bodies Induces Apoptotic Effects on MDA-MB-231 Human Breast Cancer Cells. Nutrients, 15(14), 3091.
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Cell Line Preparation and Reagents

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Cultured MCF7-Luc (DMEM, 10% FBS), SUM225-Luc (DMEM/F12; 5% FBS, insulin 10 μg/mL, hydrocortisone 0.5 μg/mL), MDA-MB-231, and BT474 (DMEM; 10% FBS, Corning Cellgro) were used. The cell lines were authenticated within the last 1 y. The IT, HB21(Fv)-PE40, and the anti-TFR mAb, HB21, were generated in the Pastan Laboratory (National Cancer Institute, Bethesda, MD). Human serum albumin, 0.2% in PBS (HSA, Grifols Biologicals LLC, NDC 68516–5216-1) was used to dilute the IT and HB21 and was used as the vehicle control.
Wang G., Kumar A., Ding W., Korangath P., Bera T., Wei J., Pai P., Gabrielson K., Pastan I, & Sukumar S. (2022). Intraductal administration of transferrin receptor-targeted immunotoxin clears ductal carcinoma in situ in mouse models of breast cancer—a preclinical study. Proceedings of the National Academy of Sciences of the United States of America, 119(24), e2200200119.
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9

Culturing Diverse Breast Cancer Cell Lines

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Human breast cancer cell lines, MDA-MB-231, MCF7, BT-549, MDA-MB-468, and mouse fibroblast NIH-3T3 were purchased from American Type Culture Collection (ATCC, Manassas, VA). MDA-MB-231, MCF-7, NIH-3T3 cells were maintained in DMEM (Cellgro, Manassas, VA), supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies, Carlbad, CA), 100 U/mL penicillin and 100 ug/mL streptomycin (Gibco) at 37 °C in humidified 5% CO2 incubator. BT-549 cells were maintained in RPMI-1640 (Cellgro, Manassas, VA) with 0.023 IU/ml insulin, 10% FBS and 1% penicillin/streptomycin at 37 °C in humidified 5% CO2 incubator. MDA-MB-468 cells were cultured in L-15 (Cellgro, Manassas, VA) with 10% FBS, 1% of penicillin/streptomycin at 37 °C in humidified 0% CO2 incubator.
Lee T.S., Kim Y., Zhang W., Song I.H, & Tung C.H. (2018). Facile Metabolic Glycan Labeling Strategy for Exosome Tracking. Biochimica et biophysica acta, 1862(5), 1091-1100.
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Culturing Common Cell Lines

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MCF10A, MCF7, T47D, DU4475, MDA-MB-231, BT549, and 293T cell lines were obtained from the American Type Culture Collection. MCF10A cells were cultured as previously described [55 (link)] in DMEM/F12 supplemented with 5% horse serum, 20 ng/mL EGF, 0.5 mg/mL hydrocortisone, 100 ng/mL cholera toxin, 10 mg/mL insulin and pen/strep. MCF7, MDA-MB-231 and 293T cells were cultured in DMEM containing 10% FBS (Corning). T47D, DU4475 and BT549 cells were cultured in RPMI-1640 medium supplemented with 10% FBS.
Zhang J., Shao X., Sun H., Liu K., Ding Z., Chen J., Fang L., Su W., Hong Y., Li H, & Li H. (2016). NUMB negatively regulates the epithelial-mesenchymal transition of triple-negative breast cancer by antagonizing Notch signaling. Oncotarget, 7(38), 61036-61053.
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