Whole hearts of TIP30 WT and Het mice were perfused and fixed in 150 mM HEPES buffer, pH 7.35, containing 1.5% paraformaldehyde and 1.5% glutaraldehyde over night. 2‐mm cubes of heart tissue were then washed in 0.15 M HEPES buffer (2 × 6 min) and 0.1 M cacodylate buffer, pH 7.35 (4 × 6 min), postfixed in 1% osmium tetroxide in cacodylate buffer (2 h), followed by washing steps (4 × 5 min cacodylate buffer, 2 × 5 min water) and 4% aqueous uranyl acetate (over night at 4°C). The heart tissue was then washed in water (2 × 5 min), dehydrated in acetone, and embedded in Epon. 50‐nm sections were poststained with 4% uranyl acetate and lead citrate. Electron microscopic examinations were performed by a blinded observer with a FEI Morgagni 268 transmission electron microscope (FEI, Eindhoven, Netherlands) operated at 80 kV using a Veleta CCD camera (Olympus Soft Imaging Solutions).
Veleta ccd camera
Manufactured by Olympus
Sourced in Germany, Japan, Netherlands, United States
The Veleta CCD camera is a high-performance scientific imaging device designed for laboratory applications. It features a large-format CCD sensor that delivers high-resolution, low-noise images. The Veleta CCD camera is capable of capturing detailed visual data for a wide range of scientific and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Tecnai spirit, by Thermo Fisher Scientific (6 mentions)
Tecnai g2 spirit biotwin, by Thermo Fisher Scientific (5 mentions)
Em uc6 ultramicrotome, by Leica (5 mentions)
Item software, by Olympus (5 mentions)
Leo 912ab omega electron microscope, by Zeiss (5 mentions)
Morgagni 268 tem, by Thermo Fisher Scientific (4 mentions)
Tia software, by Thermo Fisher Scientific (4 mentions)
Morgagni 268 transmission electron microscope, by Thermo Fisher Scientific (3 mentions)
Em uc7 ultramicrotome, by Leica (3 mentions)
Agar 100 resin, by Agar Scientific (3 mentions)
Tecnai g2 tem, by Olympus (3 mentions)
Pelco easiglow, by Ted Pella (3 mentions)
7100 electron microscope, by Hitachi (2 mentions)
Ultrostain 2, by Leica (2 mentions)
Tecnai 12 spirit bio twin, by Thermo Fisher Scientific (2 mentions)
Em uc7, by Leica (2 mentions)
Durcupan acm resin, by Merck Group (2 mentions)
Formvar carbon coated copper grid, by Ted Pella (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Formvar carbon coated 200 mesh copper grids, by Electron Microscopy Sciences (2 mentions)
82 protocols using veleta ccd camera
1
Ultrastructural Analysis of Mouse Hearts
2
Freeze Fracture Replica Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Freeze fracture replicas were produced using a previously described method (Tarusawa et al., 2009 (link)) with some modifications. Cells (2 × 105 cells/ml) were seeded onto polyethylene terephthalate filters with 0.4-µm pore size (#353090; Corning) and cultured for 6 d. The cells were washed with 0.1 M phosphate buffer (PB) and fixed with 2% glutaraldehyde in 0.1 M PB at 4°C overnight. After washing with 0.1 M PB, the samples were cryoprotected with 30% glycerol in 0.1 M PB at 4°C overnight, and then rapidly frozen in between two copper carriers using a high-pressure freezing machine (HPM010; BAL-TEC). The cells were then fractured by separation of the two carriers at −120°C and replicated by platinum (45° unidirectional from horizontal level, 2 nm thick) and carbon (20 nm thick) in a freeze-fracture replica machine (BAF060; BAL-TEC). The replicated materials were transferred to a solution containing kitchen bleach (50%) and incubated with shaking until cell debris was removed from the replicas. The replicas were washed twice with distilled water and picked up onto grids coated with Pioloform (Agar Scientific). The samples were observed with a JEM1010 transmission EM (JEOL) at 100 kV accelerating voltage. Images were captured with a Veleta CCD camera using iTEM software (Olympus Soft Imaging Solutions). All freeze-fracture images are presented apical-side up in the figures.
3
Electron Microscopy of Mouse Lymph Nodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mLNs were fixed by immersion in 150 mM HEPES, pH 7.35, containing 1.5% formaldehyde and 1.5% glutaraldehyde. After overnight incubation at 4 °C with 1% OsO4 (2 h at RT) and 4% uranyl acetate, the mLNs were dehydrated in acetone and embedded in Epon. Subsequently, 50-nm sections were poststained with uranyl acetate and lead citrate (48) and observed with a Morgagni TEM (FEI, Eindhoven, Netherland). Images were captured with a side-mounted Veleta CCD camera (Olympus Soft Imaging Solutions, Münster, Deutschland).
4
Ultrastructural Analysis of CR.pIX Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single-cell cultures (not suspended aggregates) of 20 mL × 2 × 106 CR.pIX cells/mL were infected with MOI of 8 and cultivated for 30 h. The infected cells were collected by centrifugation for 5 min with 200 ×g and resuspended in 2.5% glutaraldehyde in 100 mmol/L phosphate buffer, pH 7.3, and stored at 4 °C until further processing. The fixed cells were rinsed in 100 mmol/L phosphate buffer, pH 7.4 and then treated with 2% osmium tetroxide in 100 mmol/L phosphate buffer, pH 7.4 at 4 °C for 2 h. They were next dehydrated in ethanol and acetone prior to embedding in LX-112 (Ladd, Burlington, Vermont, USA). Ultrathin sections (approximately 50-60 nm) were prepared using a Leica ultracut UCT (Leica, Wien, Austria) and contrasted with uranyl acetate followed by lead citrate. The ultrathin sections were examined in a Tecnai G2 Spirit BioTWIN transmission electron microscope (FEI Company, Eindhoven, The Netherlands) and digital images were acquired using a 2 k × 2 k side-mounted Veleta CCD camera (Olympus Soft Imaging Solutions, GmbH, Münster, Germany).
5
Ultrastructural Analysis of Spinal Cord
6
Ultrastructural Analysis of Larval Ovaries
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LL3 ovaries were dissected from larvae in EBR (130 mM NaCl, 5 mM KCl, 2 mM CaCl2, 10 mM HEPES [pH 6.9]), and then fixed for several hours at room temperature in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.3). After the samples were rinsed three times for 5 min each in 0.1 M sodium cacodylate buffer containing 3% sucrose, they were fixed again for 1 h on ice in 1% OsO4 in 0.1 M sodium cacodylate buffer. After the samples were rinsed three times for 5 min each in ice-cold distilled water, they were stained en bloc with 0.5% aqueous uranyl acetate for 1 h on ice, and then dehydrated in an ethanol series (50%, 70%, 90% for 5 min each on ice, and then 99.5% three times for 5 min each at room temperature). The samples were then embedded in Epon 812 (TAAB) and cured at 70°C for 3 d. The embedded samples were cut into semi-thin sections (1 µm thick), stained with toluidine blue to select the areas of interest, and then cut into ultrathin sections (70–80 nm). These ultrathin sections were collected on copper grids and stained with 2% uranyl acetate and lead citrate [30] (link). Electron micrographs were obtained using a VELETA CCD camera (Olympus Soft Imaging Solutions) mounted on a JEM-1010 electron microscope (Jeol Ltd.)
7
Ultrastructural Analysis of Dorsal Horn
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For electron microscopy, after development of the immunoperoxidase reaction, sections were first treated with 1% OsO4 in 0.1 m PB for 10 min in the dark, on ice, and then dehydrated in an ascending series of ethanol solutions, followed by acetonitrile. An additional treatment with uranyl acetate (1% in 70% ethanol for 10 min in the dark, on ice) was included during the dehydration process. Sections were embedded in Durcupan (ACM, Fluka, Buchs, Switzerland). Areas of interest containing the dorsolateral fasciculus (Lissauer’s tract) and the superficial laminae were cut from the dorsal horn of lumbar segments of both MGL+/+ and MGL−/− spinal cords, and re-sectioned to produce ultrathin 50-nm thin sections with a Leica EM UC6 Ultramicrotome (Leica Microsystems). These sections were collected on a Formvar-coated single-slot copper grid, contrasted with lead citrate (Ultrostain2; Leica), and examined with a Hitachi 7100 electron microscope (Hitachi High-Technologies, Tokyo, Japan). Electron micrographs at 40 000 × magnification were acquired with a Veleta CCD camera (Olympus Soft Imaging Solutions, Munster, Germany).
8
Liposome Morphology Characterization by TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) was used to examine the morphology of the manufactured liposomes. The sample preparation using the negative staining technique was slightly modified from the method developed by Ruozi et al. [49 (link)]. Liposome samples were diluted 20 times before placing a small droplet (~80 µL) on the parafilm. A 200-mesh formvar copper grid (Agar Scientific Ltd., Essex, UK) was placed into the liposome droplet for 4 min. To stain the grid, a drop of 2% uranyl acetate was placed on the parafilm and the grid (coated with liposomes) was incubated in the solution for 4 min. The stained copper grid needed approximately 2 min for drying at room temperature. Finally, the microstructure of the liposomes was seen under TEM (FEI Tecnai G2 Spirit BioTWIN, Brno-Černovice, Czech Republic) at 100 kV. A Veleta CCD camera (Olympus Soft Imaging Solutions, Munster, Germany) was used to capture the images.
9
Visualizing Amyloid-β Fibrils with TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5 μL of Aβ42 fibrils with
or without Cu(II), obtained directly after the aggregation kinetics
experiments, was spotted on formvar/carbon coated 400 mesh copper
TEM grids. After 10 min, the excess sample was removed with blotting
paper. Then, the grid was washed twice with MQ water followed by staining
with 1% aqueous uranyl acetate solution for 5 min. The grid was air-dried
and then TEM imaging (FEI Tecnai 12 Spirit BioTWIN, operated at 100
kV) was performed. Images were recorded using a 2 k × 2 k Veleta
CCD camera (Olympus Soft Imaging Solutions, GmbH, Münster,
Germany). Images were recorded at magnification of 43,000×.
or without Cu(II), obtained directly after the aggregation kinetics
experiments, was spotted on formvar/carbon coated 400 mesh copper
TEM grids. After 10 min, the excess sample was removed with blotting
paper. Then, the grid was washed twice with MQ water followed by staining
with 1% aqueous uranyl acetate solution for 5 min. The grid was air-dried
and then TEM imaging (FEI Tecnai 12 Spirit BioTWIN, operated at 100
kV) was performed. Images were recorded using a 2 k × 2 k Veleta
CCD camera (Olympus Soft Imaging Solutions, GmbH, Münster,
Germany). Images were recorded at magnification of 43,000×.
10
Ultrastructural Analysis of hPSC-RPE Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
hPSC-RPE cells were grown on transwell inserts coated with LN521 (20 μg/mL) for 60 days. They were fixed by immersion in 2.5% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4. The transwell membrane was cut out and into thin strips, rinsed in 0.1 M phosphate buffer, followed by post fixation in 2% osmium tetroxide in 0.1 M phosphate buffer, pH 7.4, at 4 °C for 2 h. The membrane strips were subjected to stepwise ethanol dehydration and finally flat embedded in LX-112. Ultrathin sections (~50–60 nm) were prepared using a Leica EM UC7 and contrasted with uranyl acetate, followed by lead citrate. Transmission electron microscopy imaging was done on a Hitachi HT7700 transmission electron microscope (Hitachi High-Technologies) operated at 80 kV and digital images were acquired using a Veleta CCD camera (Olympus Soft Imaging Solutions).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!