Male c57bl 6 mice

Male C57/Bl6 mice purchased from Taconic (Ry, Denmark) were used at the age of 10–11 weeks. Mice fasted overnight (12 h) were injected intraperitoneally (i.p.) with apoAI-WT or apoAI-M (14 mg/kg) (control animals received NaCl). Blood samples were collected three hours following treatment. Serum samples (2 µL) were separated by SDS-PAGE, in the presence or absence of the reducing agent dithiothreitol (Sigma), and transferred to nitrocellulose membranes, probed with anti-human apoA-I antibodies (Abcam) and immune detection performed with HRP-conjugated secondary antibodies (GE Healthcare). Blots were imaged using the Odyssey Fc system (LI-COR) and quantified using Image studio v2.0 software. The animal procedures were approved by the Malmö/Lund Committee for Animal Experiment Ethics.

Male C57BL/6 mice (Taconic Farms) were housed in high efficiency particulate air (HEPA) filtered cages with sterilized bedding and fed autoclaved chow and water ad libitum throughout the experiment. Prior to antibiotic treatment, mouse faecal pellets were plated onto Sabouraud agar containing chloramphenicol and streptomycin to verify that the mice were not colonized with Candida spp. The mice were divided into a control and two treatment groups. The following antibiotics were added to the drinking water of mice in the two treatment groups and continued for the duration of the experiment: a) vancomycin (0.5 mg/ml), and b) a combination of penicillin (1.5 mg/ml), streptomycin (2 mg/ml), and gentamicin (0.1 mg/ml) (PSG) (Figure 1). After 7 days of antibiotic treatment, 10⁵ yeast cells of C. albicans SC5314 were administered by gavage to some of the mice. Others were continued on antibiotic treatment alone and were not colonized. All colonized mice were quarantined to prevent cross-infection. The mouse studies were carried out in accordance with the National Institutes of Health guidelines for the ethical treatment of animals. This protocol was approved by the Institutional Animal Care and Use Committee of the Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center.

Male C57BL/6 mice (8–12 wks old) were purchased from Taconic Farms (Germantown, NY, USA). Animals were allowed to acclimate for at least one week prior to experimentation. All animals were housed under standard conditions (room temperature 22°C with a 12h light–dark cycle) with access to chow and water. All animal experiments were performed in accordance with the National Institutes of Health Guidelines and according to protocol #2009–002, which was approved by the Institutional Animal Care and Use Committee (IACUC) of the Feinstein Institute for Medical Research at Northwell Health. The LLC-PK₁ porcine renal epithelial, MCF7 human differentiated breast cancer and CT26 mouse colon carcinoma cell lines were purchased from ATCC (Manassas, VA, USA). The H460 human large cell lung cancer cell line was provided by Dr. H Simpkins (The Feinstein Institute for Medical Research, Manhasset, NY, USA).

Male C57Bl/6 mice (22–30 g, Taconic Farms) or male Fischer 344 rats (250–350 g, Taconic Farms) were used for all experiments. The animals were group housed at room temperature and kept on a 12∶12 hour light:dark cycle with ad libitum access to water and food. The animals were habituated to the experimental room for one day before testing and at least two hours before each experiment. All experiments were approved by the Stony Brook University Institutional Animal Care and Use Committee. Unless otherwise stated, animals were euthanized with CO₂ and subsequently decapitated. The experimenter was blinded to the treatment condition of each animal.