Proximity ligation assay (PLA) was performed using the anti-Mouse MINUS probe, anti-Rabbit PLUS probe and the orange detection reagent (Sigma-Aldrich) as previously described(53 (link)). Actin was visualized using actistain 670 and imaged on a Leica scanning confocal (SP5) with a HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective. The total number of distinct PLA spots per cell were quantified using the threshold function of ImageJ.
Hcx plan apochromat 63 1.40 0 60 na oil λ blue objective
Manufactured by Leica
The HCX Plan Apochromat 63×/1.40–0.60 NA oil λ blue objective is a high-performance microscope objective designed for use with Leica microscopes. It provides a magnification of 63x and a numerical aperture range of 1.40–0.60, making it suitable for a variety of imaging applications. The objective is optimized for use with oil immersion and is compatible with the blue light spectrum.
Automatically generated - may contain errors
2 protocols using hcx plan apochromat 63 1.40 0 60 na oil λ blue objective
1
Proximity Ligation Assay for Protein Interactions
2
Proximity Ligation Assay for Protein Interactions
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