Rat anti-mouse CD45 (clone 30-F11, catalog no. 5505539, 1/400 dilution), CD11b (clone M1/70, catalog no. 550282, 1/400 dilution), and hamster anti-mouse CD11c (clone HL3, catalog no. 550283, 1/50 dilution) were purchased from BD Biosciences (San Jose, CA); F4/80 (clone CI:A3–1, catalogno. MCA497R, 1/400 dilution) and from Bio-Rad Laboratories (Hercules, CA). Goat anti-rat IgG-Alexa Fluor 546 (AF546) (catalog no. A11081, 1/1000 dilution) and goat anti-hamster IgG-AF546 (catalog no. A21111, 1/1000 dilution) were purchased from Thermo Fisher Scientific (Grand Island, NY).
Goat anti rat igg alexa fluor 546 af546
Manufactured by Thermo Fisher Scientific
Goat anti-rat IgG-Alexa Fluor 546 (AF546) is a secondary antibody conjugate used in fluorescence-based applications. It is generated by conjugating goat anti-rat IgG antibodies with Alexa Fluor 546 dye. The Alexa Fluor 546 dye provides a bright, red-orange fluorescent signal that can be detected using appropriate filter sets.
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2 protocols using goat anti rat igg alexa fluor 546 af546
1
Multicolor Flow Cytometry Immunophenotyping
2
Multicolor Flow Cytometry Immunophenotyping
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Rat anti-mouse CD45 (clone 30-F11, catalog no. 5505539, 1/400 dilution), CD11b (clone M1/70, catalog no. 550282, 1/400 dilution), and hamster anti-mouse CD11c (clone HL3, catalog no. 550283, 1/50 dilution) were purchased from BD Biosciences (San Jose, CA); F4/80 (clone CI:A3–1, catalogno. MCA497R, 1/400 dilution) and from Bio-Rad Laboratories (Hercules, CA). Goat anti-rat IgG-Alexa Fluor 546 (AF546) (catalog no. A11081, 1/1000 dilution) and goat anti-hamster IgG-AF546 (catalog no. A21111, 1/1000 dilution) were purchased from Thermo Fisher Scientific (Grand Island, NY).
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