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Transblot sd

Manufactured by GE Healthcare
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The TransBlot SD is a semi-dry electrophoretic transfer cell used for the efficient transfer of proteins from polyacrylamide gels to membranes. It provides a simple and reliable method for western blotting applications.

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Lab products found in correlation

Illustra g 25 microspin columns, by GE Healthcare (2 mentions) Semi dry transfer system, by Bio-Rad (2 mentions) Hybond n membrane, by GE Healthcare (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Phosphor screen, by GE Healthcare (2 mentions) Ultrahyb oligo, by Thermo Fisher Scientific (2 mentions) T4 polynucleotide kinase, by New England Biolabs (2 mentions) Hybond, by Cytiva (2 mentions)

2 protocols using transblot sd

1

Northern Blot Analysis of RNA

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20 μg of total RNA isolated with Trizol Reagent (Life Technologies) was separated on a 12% polyacrylamide/Urea/TBE gel and transferred to HyBond N+ membrane (GE Healthcare) using the TransBlot SD. Semi-dry Transfer System (Bio-Rad). DNA oligo probes were γ-32P end labeled using T4 polynucleotide kinase (New England Biolabs) and purified using Illustra G-25 MicroSpin columns (GE Healthcare). Blots were blocked in UltraHyb Oligo (Life Technologies) at 42°C for 30 min and hybridized with 5′ labeled DNA probe overnight at 42°C. Blots were washed twice with pre-warmed 2xSSC and 0.5% SDS at 42°C for 30 minutes. RNA was visualized with phosphor screens from GE-HealthCare.
Kelly T.J., Brümmer A., Hooshdaran N., Tariveranmoshabad M, & Zamudio J.R. (2019). Temporal Control of the TGF-β Signaling Network by Mouse ESC MicroRNA Targets of Different Affinities. Cell reports, 29(9), 2702-2717.e7.
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2

Northern Blot Analysis of RNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 μg of total RNA isolated with Trizol Reagent (Life Technologies) was separated on a 12% polyacrylamide/Urea/TBE gel and transferred to HyBond N+ membrane (GE Healthcare) using the TransBlot SD. Semi-dry Transfer System (Bio-Rad). DNA oligo probes were γ-32P end labeled using T4 polynucleotide kinase (New England Biolabs) and purified using Illustra G-25 MicroSpin columns (GE Healthcare). Blots were blocked in UltraHyb Oligo (Life Technologies) at 42°C for 30 min and hybridized with 5′ labeled DNA probe overnight at 42°C. Blots were washed twice with pre-warmed 2xSSC and 0.5% SDS at 42°C for 30 minutes. RNA was visualized with phosphor screens from GE-HealthCare.
Kelly T.J., Brümmer A., Hooshdaran N., Tariveranmoshabad M, & Zamudio J.R. (2019). Temporal Control of the TGF-β Signaling Network by Mouse ESC MicroRNA Targets of Different Affinities. Cell reports, 29(9), 2702-2717.e7.
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