All reagents used for the experiments were purchased from Sigma-Aldrich (Munich, Germany) and Merck Co. (Darmstadt, Germany) and used without further purification. They had a class of the purity declared by the manufacturer. The melting points of the obtained compounds were determined with a Fisher–Johns apparatus (Fisher Scientific, Schwerte, Germany), without any correction. The purity of the compounds obtained was assessed through thin layer chromatography (TLC) on plates covered with silica gel (aluminum oxide 60 F-254) by Merck. Chloroform-ethanol mixtures in the ratio 10:1 (v/v) were used as the mobile phase. Spots were developed by irradiation with UV light with a wavelength λ = 254 nm. FT-IR spectra were recorded on a Nicolet 6700 spectrometer (Thermo Scientific, USA); in cm−1. 1H and 13C NMR spectra were recorded on the Bruker Avance 300 and 600 apparatus (Bruker BioSpin GmbH, Hamburg, Germany). The compounds were dissolved in dimethyl sulfoxide (DMSO-d6) for analysis. Tetramethylsilane (TMS) was used as an internal standard. Chemical shift values are given in ppm. Then elemental analyses were determined by a Perkin Elmer 2400 series II CHNS/O analyzer (PerkinElmer, Waltham, MA, USA), and the results were within ±0.4% of the theoretical value.
Perkin elmer 2400 series 2 chns o analyzer
Manufactured by PerkinElmer
Sourced in United States
The PerkinElmer 2400 Series II CHNS/O Analyzer is a laboratory instrument designed to perform elemental analysis. It is capable of determining the carbon, hydrogen, nitrogen, sulfur, and oxygen content in a wide range of organic and inorganic samples.
Automatically generated - may contain errors
Lab products found in correlation
Aluminum oxide 60 f 254, by Merck Group (1 mentions)
Nicolet 6700 spectrometer, by Thermo Fisher Scientific (1 mentions)
Fisher johns apparatus, by Thermo Fisher Scientific (1 mentions)
Avance 300 and 600, by Bruker (1 mentions)
S 4300 e n fesem, by Hitachi (1 mentions)
Countess 2 fl automated cell counter, by Thermo Fisher Scientific (1 mentions)
Technics hummer 5 sputter coater, by Anatech (1 mentions)
2.2.2 cryptand crypt 222, by Merck Group (1 mentions)
Tetrabutylammonium hexafluorophosphate nbu4npf6, by Merck Group (1 mentions)
5 protocols using perkin elmer 2400 series 2 chns o analyzer
1
Synthesis and Characterization of Organic Compounds
2
Characterization of Allene Derivatives
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Melting points were determined on a Büchi apparatus (Büchi, Flawil, CH) in open tubes and are uncorrected. IR spectra were recorded on a Perkin-Elmer 1725 X spectrophotometer (Perkin-Elmer, Waltham, MA, USA). Element analyses were taken on a Perkin-Elmer 2400, Series II CHNS/O Analyzer (Perkin-Elmer, Waltham, MA, USA). Mass spectra were determined on a VG-70EQ apparatus (Waters, Milford, MA, USA). 1H-NMR (300 MHz) and 13C-NMR (75 MHz) spectra were taken with a Bruker Fourier 300 instrument (in CDCl3 solutions at room temperature) (Bruker Corporation, Billerica, MA, USA). Chemical shifts are given as parts per million from tetramethylsilane. Coupling constants (J) values are given in hertz and are quoted to ±0.1 Hz consistently with NMR machine accuracy. NOESY experiments were performed by setting the following parameters: relaxation delay (d1) 2 s, irradiation power (dl2) 74 dB and total irradiation time (for each signal) 1.8 s.
The following compounds are known in the literature: methoxycarbonylallene1a : Ref. [16 ]; sulfonylallene 1b : Ref. [45 (link)]; tetramethylallene 2a : Ref. [43 (link)]; tetrafluoroallene 2b : Ref. [44 (link)]; arylazides 3a –c : Ref. [8 (link)].
The following compounds are known in the literature: methoxycarbonylallene
3
Characterizing Pollen Shell Interior
4
Inert Atmosphere Synthesis Protocols
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All manipulations were performed
in either an argon- or nitrogen-filled MBraun glovebox with an atmosphere
of <0.1 ppm of O2 and <0.1 ppm of H2O.
House nitrogen was purified through an MBraun HP-500-MO-OX gas purifier.
Tetrahydrofuran (THF) was predried by refluxing over potassium for
several days, distilled, and stirred over Na/K alloy for final purification.
Dimethoxyethane (DME) and toluene were dried over potassium, and nhexane was dried over calcium hydride. All
solvents were distilled under N2 from their drying agents,
and water/oxygen absence was confirmed via benzophenone solution as
an indicator within a glovebox prior to use. Deuterated solvents were
purchased from Cambridge Isotope Laboratories, dried over Na/K alloy
for several days, and filtered prior to use. Anhydrous ErCl3 was purchased from Sigma-Aldrich and used as received. 2.2.2-Cryptand
(crypt-222) was purchased from Sigma-Aldrich and crystallized from
hot nhexane prior to use. Tetrabutylammonium
hexafluorophosphate [nBu4N][PF6] was purchased from Sigma-Aldrich and crystallized several
times before use. Dibenzocyclooctatetraene (dbCOT)40 (link) and potassium graphite (KC8)41 (link) were prepared according to literature procedures. Elemental
analysis was performed at Michigan State University using a PerkinElmer
2400 Series II CHNS/O analyzer.
in either an argon- or nitrogen-filled MBraun glovebox with an atmosphere
of <0.1 ppm of O2 and <0.1 ppm of H2O.
House nitrogen was purified through an MBraun HP-500-MO-OX gas purifier.
Tetrahydrofuran (THF) was predried by refluxing over potassium for
several days, distilled, and stirred over Na/K alloy for final purification.
Dimethoxyethane (DME) and toluene were dried over potassium, and nhexane was dried over calcium hydride. All
solvents were distilled under N2 from their drying agents,
and water/oxygen absence was confirmed via benzophenone solution as
an indicator within a glovebox prior to use. Deuterated solvents were
purchased from Cambridge Isotope Laboratories, dried over Na/K alloy
for several days, and filtered prior to use. Anhydrous ErCl3 was purchased from Sigma-Aldrich and used as received. 2.2.2-Cryptand
(crypt-222) was purchased from Sigma-Aldrich and crystallized from
hot nhexane prior to use. Tetrabutylammonium
hexafluorophosphate [nBu4N][PF6] was purchased from Sigma-Aldrich and crystallized several
times before use. Dibenzocyclooctatetraene (dbCOT)40 (link) and potassium graphite (KC8)41 (link) were prepared according to literature procedures. Elemental
analysis was performed at Michigan State University using a PerkinElmer
2400 Series II CHNS/O analyzer.
5
Comprehensive Compost Characterization Protocol
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Temperature was measured at a depth of 60 cm from the top of pile using digital thermometer with long-handled stainless-steel probe in the four edges and center of the pile. Moisture content determination was performed by microwave oven drying as previously described63 . The pH was determined from suspensions of fresh compost samples in 0.9% sodium chloride using a pH electrode. Total Carbon, Hydrogen and Nitrogen content were determined using PerkinElmer 2400 series II CHNS/O analyzer (Perkin-Elmer, USA). Elemental analysis (Al, Fe, Mg, P, As, Cd, Cr, Cu, K, Ni, Pb, Se and Zn) was performed by inductively-coupled plasma optical emission spectrometry using Spectro Arcos ICP-OES analyzer (Spectro, Germany).
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