Triglyceride monostearate (TG-18) was purchased from AK Scientific (USA). Rosiglitazone (R2408) (molecular weight: 357.43 g/mol) and matrix metalloproteinase nine (MMP-9) (PF024) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-NF-κB (Cat#8242), mouse anti-p-NF-κB (Cat#3036) and rabbit anti-GAPDH (Cat#5174) were from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-adiponectin (ab22554) and rabbit anti-PPARγ (ab59256) were from Abcam (Cambridge, UK). Mouse anti-cytokeratin 14 (sc-53253) was from Santa Cruz Biotechnology (CA, USA). Rabbit anti-MMP-3 (GB11131) and anti-MMP-9 (GB11132) were from Servicebio (Wuhan, China). LIVE/DEAD® Kit Contents Viability/Cytotoxicity Assay Kit (L3224), Alexa Fluor 594-conjugated IgG (A11058), Alexa Fluor 488-conjugated IgG (A11055, A21206), 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine Perchlorate (DiD) (D307) and BCA protein assay kit (23225) were from Thermo Fisher Scientific (USA). 40,6-diamidino-2-phenylindole (DAPI, H-1200) was from Vector (Burlingame, CA, USA). Quantibody Mouse Cytokine Array 2 was from Ray Biotech (Norcross, GA, USA).
Rosiglitazone r2408
Manufactured by Merck Group
Sourced in United States
Rosiglitazone (R2408) is a pharmaceutical compound developed by Merck Group. It is a synthetic thiazolidinedione derivative. The core function of Rosiglitazone is to act as a peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonist.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit 23225, by Thermo Fisher Scientific (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Indomethacin i7378, by Merck Group (1 mentions)
Microplate reader, by Thermo Fisher Scientific (1 mentions)
Cck 8 solution, by Beyotime (1 mentions)
Gw9662, by Selleck Chemicals (1 mentions)
6 protocols using rosiglitazone r2408
1
Triglyceride Monostearate and Rosiglitazone-Mediated Anti-Inflammatory Effects
2
Adipocyte Differentiation Assay
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Tissue culture media were from Biological Industries (Beit-Haemek, Israel). Indomethacin (I7378), dexamethasone (D4902), 3-isobutylmethylxanthine (IBMX) (I7018), and rosiglitazone (R2408) were from Sigma–Aldrich. Recombinant murine/human TNF (410-MT, 210-TA respectively) and murine IL-1β (401-ML) were purchased from R&D Systems Inc.
3
Cell Viability Assay with Rosiglitazone and GW9662
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The cells were placed in 96-well plates at a density of 5 × 103 cells/well and cultured overnight. Then, the cells were cultured with a complete medium containing different concentrations of rosiglitazone (R2408, Sigma-Aldrich; Merck KGaA) or GW9662 (No.S2915, Selleck Chemicals, Houston, TX, USA) for 24, 48, and 72 h. After 24, 48 and 72 h, cells were incubated with CCK8 solution (10 μl/well, Beyotime Biotechnology, no. C0038) for 1 h at 37 °C. The absorbance was measured at OD 450 nm by a microplate reader (Thermo Fisher Scientific Inc.).
4
Osteoblast Differentiation Antibodies
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Anti-C/EBPβ (04-1153) and anti-C/EBPα (04-1104) antibodies were purchased from Upstate Biotechnologies (Lake Placid, NY, USA). Anti-Osterix (sc-22538), anti-Myc (sc-40), anti-HA (sc-57592), and anti-α-tubulin (sc-53646) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-PPARγ was purchased from Chemicon International Inc. (MAB3872, Temecula, CA, USA). Insulin (I2643), dexamethasone (D4902), 3-isobutyl-1-methylxanthine (I5879), and rosiglitazone (R2408) were purchased from Sigma (St. Louis, MO).
5
Thiazolidinedione Compounds Acquisition
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Rosiglitazone (R2408), pioglitazone (E6910), and nTZDpa (SML0616) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Troglitazone (209-19481) was purchased from FUJFILM Wako Pure Chemical Corporation (Osaka, Japan). Sodium acetate buffer solution (06893-24) was purchased from Nacalai Tesque (Kyoto, Japan).
6
Rosiglitazone: PPAR-gamma agonist effects
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Rosiglitazone (R2408) (ROSI, A PPARγ pathway agonist) and DMSO (D2650) were purchased from Sigma (St. Louis, MO, USA). For culture experiments, 10 µM ROSI was dissolved in DMSO, which was further diluted in RPMI1640 medium and added to the culture to a final DMSO concentration of 0.1%.
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