S aureus atcc 25923

S. aureus (ATCC 25923) and MRSA (ATCC 43300) strains were obtained from China Center of Industrial Culture Collection. It is usually acknowledged that the logarithmic phase cells are more sensitive to external stress factors, while stationary phase cells are more resistant [33 ,34 (link)]. The bacteria strains were cultured overnight to the logarithmic phase of growth at 37 °C (approximately 16 h) in Luria-Bertani broth culture medium (AOBOX Biotechnology, Beijing, China). Both inoculums were measured at OD600 and adjusted to the concentration of 5 × 10⁷ CFU/mL using the McFarland standard. Culture purity was examined by streaking each culture on plates of LB agar and nutrient agar for S. aureus and MRSA and incubating at 37 °C overnight.

10-HDA (≥98%) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). The standard strain S. aureus ATCC25923 was purchased from the China Center of Industrial Culture Collection (Beijing, China). 10-HDA was dissolved in dimethyl sulfoxide (DMSO) (0.5%, v/v) and serially diluted to different concentrations by Tryptic Soytone Broth (TSB) liquid medium.

Agar, gentamicin sulfate (AR), ethylene glycol (AR), sodium cyanoborohydride and sodium periodate (AR) were all purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). E. coli (CMCC(B) 44103) and S. aureus (ATCC 25923) were all obtained from China Center of Industrial Culture Collection, Beijing, China. Tryptic soy Agar (TSA) and Tryptone soya broth (TSB) were obtained from Beijing Land Bridge Technology Co., Ltd. (Beijing, China).