Total RNA was extracted with the Absolutely RNA RT-PCR microprep kit (Agilent Technologies, Santa Clara, CA, USA). The quality and quantity of the total RNA was checked by spectrophotometry. The RNA was reverse transcribed by the high-capacity complementary DNA reverse transcription kit (Roche Applied Science, Basel, Switzerland). Taqman probe for RSPO1 was used (Applied Biosystems, Grand Island, NY, USA). Real-time PCR was performed with a 7500 Real-Time PCR System (Applied Biosystems) using the following parameters: 2 min at 50 °C, 10 min at 95 °C, then 40 cycles of 15 s at 95 °C and 1 min at 60 °C. The results are presented as relative gene expression compared with the internal control 18S using the 2−ΔΔCt method. Determination was made in triplicate from three separate samples.
Taqman probe for rspo1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TaqMan probe for RSPO1 is a molecular assay designed for the detection and quantification of the RSPO1 gene. It utilizes real-time PCR technology to provide accurate and reliable results. The probe specifically targets the RSPO1 sequence, enabling researchers to study its expression and role in various biological processes.
Automatically generated - may contain errors
3 protocols using taqman probe for rspo1
1
Quantitative Analysis of RSPO1 Gene Expression
2
Quantitative RT-PCR Analysis of RSPO1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted with the Absolutely RNA RT-PCR microprep kit (Agilent Technologies, Santa Clara, CA, USA). The quality and quantity of the total RNA was checked by spectrophotometry. The RNA was reverse transcribed by the high capacity complementary DNA reverse transcription kit (Roche Applied Science, Basel, Switzerland). Taqman probe for RSPO1 was used (Applied Biosystems, Grand Island, NY, USA) Real-time PCR was performed with a 7500 Real-Time PCR System (Applied Biosystems) using the following parameters: 2 minutes at 50°C, 10 minutes at 95°C, then 40 cycles of 15 seconds at 95°C and 1 minute at 60°C. The results are presented as relative gene expression compared with the internal control 18S using the 2 -ΔΔCt method. Determination was made in triplicate from three separate samples.
3
Quantitative RT-PCR Analysis of RSPO1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted with the Absolutely RNA RT-PCR microprep kit (Agilent Technologies, Santa Clara, CA, USA). The quality and quantity of the total RNA was checked by spectrophotometry. The RNA was reverse transcribed by the high capacity complementary DNA reverse transcription kit (Roche Applied Science, Basel, Switzerland). Taqman probe for RSPO1 was used (Applied Biosystems, Grand Island, NY, USA) Real-time PCR was performed with a 7500 Real-Time PCR System (Applied Biosystems) using the following parameters: 2 minutes at 50°C, 10 minutes at 95°C, then 40 cycles of 15 seconds at 95°C and 1 minute at 60°C. The results are presented as relative gene expression compared with the internal control 18S using the 2 -ΔΔCt method. Determination was made in triplicate from three separate samples.
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