Ridaifen b

Most compounds used in the estrogen receptor library screen were synthesized in-house and have been reported.^{32 (link)–35 (link), 56 (link), 57 (link)} Toremifene, tamoxifen, raloxifene, bazedoxifene, ospemifene, ridaifen-B, nafoxidine, RAD-1901, MPP, acolbifene, and compound 7 were purchased from Sigma-Aldrich or Med Chem Express. All compounds were dissolved in DMSO at a final concentration of 10 mM and were stored at −20 °C until use.

Ridaifen-B, CP-55,940, and JWH-015 were purchased from Sigma Aldrich (St. Louis, MO). WIN-55,212-2, AM-630, and AM-281 were obtained from Tocris Bioscience (Minneapolis, MN). SR-144528 was procured from Cayman Chemicals (Ann Arbor, MI). The radioligand [³H]CP-55,940 (131.4 Ci/mmol) was purchased from Perkin Elmer (Waltham, MA) and [³⁵S]GTPγS (1250 Ci/mmol) from American Radiolabeled Chemicals (St. Louis, MO). All compounds were diluted to 10⁻²M in 100% DMSO and stored at −20 °C. N-1 napthylethlene, sulfanilamide, and lipopolysaccharide from E. coli 0111:B4 were purchased from Sigma Aldrich (St. Louis, MO). ELISA kits to measure levels of mouse TNFα and mouse IL-6 were obtained from R&D systems (Minneapolis, MN). Mouse IL-1α release was quantified by ELISA kits procured from Ray Biotech (Norcross, GA). The WST-1 cell proliferation reagent from CellPro Roche was obtained from Sigma Adrich (St. Louis, MO). Reagents for TRAP staining were purchased from Sigma Aldrich (St. Louis, MO). All other supplies were purchased from Fisher Scientific.