Anti egfr d38b1 xp

Hematoxylin and eosin (H&E) staining and periodic acid-Schiff (PAS) staining were used to evaluate fat and glycogen content respectively of liver tissue. Formalin-fixed, paraffin-embedded, dehydrated and cleared liver sections were subjected to standard procedures of immunohistochemistry ^{19 (link)}. The following primary antibodies were used for immunohistochemistry: anti-EGFR (D38B1) XP (1:50 dilution; Cell Signaling Technology, Danvers, MA) and anti-PCNA (1:100 dilution; Sigma WH0005111M2). EGFR staining was performed as described by Cell Signaling Technology EGFR antibody IHC data sheet. PCNA staining was performed using routine histological protocol. Briefly, slides were passed through xylene, graded alcohol, and rinsed in phosphate buffered saline (PBS). Liver section antigens were retrieved in sodium citrate buffer (10mM Sodium citrate, 0.05% Tween 20, pH6.0). Endogenous peroxide was inactivated using 3% hydrogen peroxide, followed by a one hour incubation at room temperature with the primary antibody, incubated in Dako EnVision+ System- HRP labeled Polymer secondary antibody (Dako North America, Inc) for 30min at room temperature. Signal was detected and developed using the Dako liquid DAB+Substrate Chromogen System (Dako North America, Inc).