The largest database of trusted experimental protocols

260 spectrophotometer

Manufactured by Shimadzu

The Shimadzu 260 spectrophotometer is a laboratory instrument designed for the measurement of light absorption by samples. It is capable of analyzing the absorbance of light at specific wavelengths within the ultraviolet and visible light spectrum.

Automatically generated - may contain errors

2 protocols using 260 spectrophotometer

1

Gastric Emptying Measurement in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gastric emptying was determined as in our previous study [33 (link)] in mice deprived of food but not water for 6–7 h during light phase. Mice had no access to food and water after the surgery and were gavaged with 0.3 ml of 1.5% methylcellulose (Sigma-Aldrich, St Louis, MO) and 0.5% phenol red (Sigma-Aldrich) and 20 min later, euthanized by cervical dislocation. The abdominal cavity was opened, gastric pylorus and cardia were clamped, the stomach removed, rinsed, placed into 15 ml of 0.1 N NaOH, homogenized for 30 s (Polytron; Brinkman Instruments, Westbury, NY). The suspension was settled for 1 h at room temperature and then 5 ml of the supernatant added to 0.5 ml of 20% trichloroacetic acid (w/v; Sigma-Aldrich). The samples were centrifuged at 3000 rpm at 4°C for 20 min. Three ml of the supernatant were mixed with 4 ml of 0.5 N NaOH. The absorbency of the sample (1 ml) was read at 560 nm (Shimadzu 260 spectrophotometer). phenol red recovered from animals euthanized immediately after the administration of the methylcellulose/phenol red solution served as a standard (0% emptying). Percentage of emptying in the 20-min period was calculated as % emptying = (1- absorbance of test sample/absorbance of standard) × 100.
+ Open protocol
+ Expand
2

Gastric Emptying of Viscous Solution

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gastric emptying of a non-nutrient meal (1.5% methylcellulose and 0.05% phenol red viscous solution) was determined as described in our previous studies [60 (link)]. In brief, rats were fasted overnight (1 rat/cage) for 18–20 h with access to water up to the start of the experiments conducted between 9:00 AM and 1:00 PM. Animals received an orogastric gavage (og) of the viscous solution (1.5 mL) and were euthanized 20 min later by CO2 inhalation followed by thoracotomy. The stomach was removed and homogenized in 100 mL of 0.1 N NaOH using a Polytron (Brinkman Instruments, Westbury, NY). Five milliliters of the supernatant were added to 0.5 mL 20% trichloroacetic acid, centrifuged at 3000 rpm at 4 °C for 20 min and 3 mL of the supernatant added to 4 mL of 0.5 N NaOH. The absorbance of the samples was read at 560 nm (Shimadzu 260 Spectrophotometer). Gastric emptying was calculated as percent emptying = (1 − absorbance of test sample/absorbance of standard) × 100. Phenol red recovered from stomach of rats euthanized immediately after gavage of the same volume of solution served as standard.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!