Freshly drawn fasting blood samples were used for analyses of comprehensive metabolic panel (automated clinical analyzer, Abbott Architect Instruments, OU Medical Center laboratory). For other assays, sera were promptly frozen at −80° C for subsequent analyses of biomarkers. Obesity-related hormones [C-peptide, ghrelin, gastric inhibitory polypeptide (GIP), glucagon, insulin, leptin, plasminogen activator inhibitor-1 (PAI-1), glucagon-like peptide-1 (GLP-1), resistin, visfatin], and inflammatory factors [IL-11, 19, 20, 26, 27, 28A, 29, 32, 34, 35, TNFSF14, Thymic stromal lymphopoietin (TSLP), osteocalcin, pentraxin-3, interferon (IFN)-β, gp130, a proliferation-inducing ligand/tumor necrosis factor superfamily 13 (APRIL/TNFSF13), B cell activating factor of the TNF family (BAFF/TNFSF13B), chitinase 3-like 1 (CHI3L1),: soluble CD30/TNF receptor superfamily member 8 (sCD30/TNFRSF8), sCD163, soluble tumor necrosis factor receptor (sTNF)-R1, R2, and TNF homologue with weak apoptosis-inducing activity (TWEAK)] were measured using Bio-Plex Pro™ magnetic bead assays (Bio-Rad Laboratories, Hercules, CA), according to the manufacturer’s instruction. High sensitivity TNF-α (hs-TNF-α) was determined using ELISA kits (R&D Systems, Minneapolis, MN) based on the manufacturer’s protocol: inter-assay CV was 7.5%.
Automated clinical analyzer
Manufactured by Abbott
The Automated Clinical Analyzer is a lab equipment product designed to perform automated analysis of clinical samples. It is capable of processing and analyzing various types of clinical samples, such as blood, urine, or other bodily fluids, to provide accurate and reliable diagnostic results.
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Lab products found in correlation
2 protocols using automated clinical analyzer
1
Comprehensive Metabolic and Biomarker Analysis
2
Comprehensive Metabolic and Inflammatory Profiling
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Freshly drawn blood samples were sent to the OU Medical Center laboratory for an analysis of a comprehensive metabolic panel, including serum glucose, lipid profiles, HbA1c, and high-sensitivity C-reactive protein (hs-CRP), using an automated clinical analyzer (Abbott Architect Instruments). Serum IL-6, IL-1β, and MMP-3 and 8 were measured using ELISA kits based on the manufacturer’s protocol (R&D Systems, Minneapolis, MN, USA) with inter-assay CVs (Coefficient of variation) of 4.5%, 7.6%, 3.6%, and 7.5%, respectively. Serum nitrite was measured using the Griess Reagent System (Promega Corporation, Madison, WI, USA) with a mean inter-assay CV of 3.3%.
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