Log In Sign up
The largest database of trusted experimental protocols

Anti mouse tcrb constant domain clone h57 597

Manufactured by BD
Visit Supplier

The Anti-mouse TCRβ constant domain (clone H57-597) is a monoclonal antibody that specifically recognizes the constant region of the mouse T cell receptor beta chain (TCRβ). This antibody is a useful tool for the identification and analysis of mouse T cells.

Automatically generated - may contain errors

Lab products found in correlation

Bovine serum albumin (bsa), by Thermo Fisher Scientific (2 mentions) Anti human hla a 02 clone bb7.2, by BD (2 mentions) Ir dye, by Thermo Fisher Scientific (2 mentions) Anti human cd8 clone rpa t8, by BD (2 mentions)

Spelling variants of this product

H57-597 (9 citations) Mouse anti (2 citations)

2 protocols using anti mouse tcrb constant domain clone h57 597

1

Flow Cytometry Analysis of Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry: anti-human CD8, (clone RPA-T8; BD biosciences), anti-human IFNγR (CD119, Clone GIR-208; eBioscience), anti-human HLA-A*02 (clone BB7.2; BD Biosciences), anti-mouse TCRb constant domain (clone H57-597; BD Biosciences) and anti-human PD-L1 (CD274, clone MIH1, eBioscience). Cell surface expression of indicated markers was assessed by staining of cells with fluorochrome-labeled antibodies in FACS buffer (0.5% w/v bovine serum albumin (Fisher Scientific) in PBS) for 20–30 min at 4 °C, while protected from light. After incubation, cells were washed twice with FACS buffer before resuspension in FACS buffer for analysis. IR-Dye (Invitrogen) was used to allow for live cell selection.
Hoekstra M.E., Bornes L., Dijkgraaf F.E., Philips D., Pardieck I.N., Toebes M., Thommen D.S., van Rheenen J, & Schumacher T.N. (2020). Long-distance modulation of bystander tumor cells by CD8+ T cell-secreted IFNγ. Nature cancer, 1(3), 291-301.
+ Open protocol
+ Expand
2

Flow Cytometry Analysis of Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry: anti-human CD8, (clone RPA-T8; BD biosciences), anti-human IFNγR (CD119, Clone GIR-208; eBioscience), anti-human HLA-A*02 (clone BB7.2; BD Biosciences), anti-mouse TCRb constant domain (clone H57-597; BD Biosciences) and anti-human PD-L1 (CD274, clone MIH1, eBioscience). Cell surface expression of indicated markers was assessed by staining of cells with fluorochrome-labeled antibodies in FACS buffer (0.5% w/v bovine serum albumin (Fisher Scientific) in PBS) for 20–30 min at 4 °C, while protected from light. After incubation, cells were washed twice with FACS buffer before resuspension in FACS buffer for analysis. IR-Dye (Invitrogen) was used to allow for live cell selection.
Hoekstra M.E., Bornes L., Dijkgraaf F.E., Philips D., Pardieck I.N., Toebes M., Thommen D.S., van Rheenen J, & Schumacher T.N. (2020). Long-distance modulation of bystander tumor cells by CD8+ T cell-secreted IFNγ. Nature cancer, 1(3), 291-301.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!