We selected several breast cancer cell lines that were isolated from primary invasive ductal breast carcinomas (IDC; BT-483 (HTB-121) and BT-474 (HTB-20), American Type Culture Collection, ATCC, Manassas, VA) [36 (link)], metastatic pleural or pericardiac effusions (AC; MCF-7 (HTB-22), MDA-MB-453 (HTB-131), and AU-565 (CRL-2351), ATCC, Manassas, VA) [37 (link)-39 (link)], or other metastatic deposits (MC; MDA-MB-231 (HTB-26), ATCC, Manassas, VA) [40 (link)]. Additionally, a continuous cell line (HBL-100, denoted as N; Cat. No. HTB-124; ATCC, Manassas, VA) obtained from a primary culture of cells derived from an early lactation sample of human milk and a normal human breast epithelial cell line (MCF-10A, denoted as F; Cat. No. CRL-10317; ATCC, Manassas, VA) were used as controls. MCF-10A cells were maintained in complete MCF-10A culture medium composed of a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 medium supplemented with 100 ng/mL cholera enterotoxin, 10 μg/mL insulin, 0.5 μg/mL hydrocortisol, and 20 ng/mL epidermal growth factor (Life Technologies, Rockville, MD, USA). MCF-7, MDA-MB-231, HBL-100, and MDA-MB-453 cells were maintained in DMEM; AU-565, BT-474 and BT-483 cells were cultured in RPMI-1640.
Cholera enterotoxin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cholera enterotoxin is a laboratory product used in research and scientific applications. It is a protein produced by the bacterium Vibrio cholerae, the causative agent of cholera. The core function of cholera enterotoxin is to induce secretory diarrhea, a characteristic symptom of cholera infection.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 10a, by Thermo Fisher Scientific (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
Insulin, by Thermo Fisher Scientific (2 mentions)
Hydrocortisol, by Thermo Fisher Scientific (2 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 453, by American Type Culture Collection (1 mentions)
Au565, by American Type Culture Collection (1 mentions)
Bt483, by American Type Culture Collection (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
2 protocols using cholera enterotoxin
1
Characterization of Breast Cancer Cell Lines
2
Culturing Breast Cancer and Normal Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human mammary gland epithelial adenocarcinoma (MCF-7 and MDA-MB-231) and human normal mammary gland epithelial fibrocystic (MCF-10A) cell lines were purchased from American Tissue Cell Culture (Manassas, VA, USA). The MCF-10A cells were maintained in complete MCF-10A culture medium; that is, a 1:1 mixture of Dulbecco's modified Eagle medium (DMEM) and Ham F12 supplemented with 100 ng/mL cholera enterotoxin, 10 μg/mL insulin, 0.5 μg/mL hydrocortisol, and 20 ng/mL epidermal growth factor (Life Technologies, Rockville, MD, USA). The MCF-7 and MDA-MB-231 cells were maintained in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS). All cell lines were grown at 37°C in a humidified 5% CO2 atmosphere. These cells were also free of mycoplasma contamination.
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