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Perifosine

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Perifosine is a synthetic organic compound that functions as an inhibitor of the phosphoinositide 3-kinase (PI3K) and Akt signaling pathways. It is used as a research tool in the study of cellular processes and disease mechanisms.

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Lab products found in correlation

Microplate reader, by Bio-Rad (1 mentions) Lapatinib, by Selleck Chemicals (1 mentions) Cck 8 agent, by Dojindo Laboratories (1 mentions) A8167, by Apexbio (1 mentions) Rapamycin, by Apexbio (1 mentions)

2 protocols using perifosine

1

Evaluating Cell Viability with CCK8 Assay

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Cell viability was determined using the CCK8 assay. First, the three groups of cells were seeded on 96-well plates at 40,000 cells/well. Following attachment, cells were incubated with different concentrations of lapatinib (S2111, Selleckchem, Houston, TX, USA) and/or perifosine (1.5–100 μM APExBIO, Houston, TX, USA) and cultured for 48 h. CCK8 agent (Dojindo Molecular Technologies, Inc., Japan) was added, and after 2 h, the absorbance at 450 nm was measured using a microplate reader (Bio-Rad Laboratories, Hercules, CA, USA).
Yang Z.Y., Yang L., Xu C.W., Wang X.J, & Lei L. (2020). An insertion mutation of ERBB2 enhances breast cancer cell growth and confers resistance to lapatinib through AKT signaling pathway. Biology Open, 9(1), bio047662.
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2

Inhibition of Akt and mTOR in N2a Cells

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Perifosine (A8309, APExBIO, USA, 10 μM, dissolved in PBS) and rapamycin (A8167, APExBIO, USA, 50 nM, dissolved in PBS) were used to inhibit Akt and mTOR, respectively. In the corresponding treatment group, each drug was added to the growth medium of N2a cells at the onset of reoxygenation.
He J., Liu J., Huang Y., Tang X., Xiao H., Liu Z., Jiang Z., Zeng L., Hu Z, & Lu M. (2021). OM-MSCs Alleviate the Golgi Apparatus Stress Response following Cerebral Ischemia/Reperfusion Injury via the PEDF-PI3K/Akt/mTOR Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2021, 4805040.
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