During behavioral testing, rats were tethered via a flexible lead to 6-channel commutators (P1 Technologies, Roanoke VA) mounted in the ceiling of the operant chambers, which allowed free movement throughout the chambers (Fig. 2B ). The commutators were connected to an 8-channel programmable constant current stimulator (STG 4000, Multi-Channel Systems, Reutlingen, Germany), which in turn was controlled by the ABET II Touch software (Campden Instruments Ltd) to allow VNS delivery at times determined by behavioral task performance of each rat. The VNS parameters used in Experiments 2.1, 2.2, and 2.4, (biphasic 0.8 s pulse train, 60 μs per phase, 700 μA, 30 Hz) were based on those previously shown to enhance cortical plasticity and extinction learning in rats (Engineer et al., 2011 (link); Loerwald, Borland, Rennaker, Hays, & Kilgard, 2018 (link); Peña et al., 2013 (link)). To determine the effects of stimulus frequency on VNS efficacy, the frequency was altered to 10 Hz and 50 Hz in Experiment 2.3.
Stg4000
Manufactured by MultiSciences Biotech
Sourced in Germany
The STG4000 is a high-performance laboratory centrifuge designed for a wide range of applications. It features a robust and durable construction, ensuring reliable operation and consistent results. The centrifuge is capable of reaching speeds up to 4,000 RPM, allowing for efficient separation of samples. The STG4000 is equipped with a temperature control system to maintain the desired sample temperature during the centrifugation process.
Automatically generated - may contain errors
3 protocols using stg4000
1
Vagus Nerve Stimulation in Rat Behavior
2
Retina Stimulation Waveform Recording
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Hantek2000 Oscilloscope (Hantek, Gyeonggi, Republic of Korea) was used for ES waveform and voltage recordings. Eight-week-old C57BL/6J mice were anesthetized with an intraperitoneal injection of Ketamine (100 mg/kg; Dechra Vet Products, Overland Park, KS, USA, 383017-01) and Xylazine (20 mg/kg; Covetrus North America, Dublin, OH, USA, 1XYL006). Under a surgical microscope, the positive probe was connected to a conductive (~ 50 Ω) 30G needle penetrating the retina, and the negative probe was connected to the negative electrode of the pulse generator STG4000 (Multichannel Systems, Reutlingen, Germany), which generated ramp, rectangular, or sine waveforms at 20 Hz and 100 µA current. The pulse generator was set in the amplitude priority (the pulse generator adjusts the voltage settings to reach 100 µA current flow depending on the tissue’s resistance). The positive probe of the pulse generator was applied to the cornea through a conductive gel interface (Spectral 360; Parker Laboratories, Fairfield, NJ, USA). The negative electrode of the pulse generator was placed on the mouse’s abdomen through the conductive gel interface to mitigate the high resistance of the fur and the skin. Thirty recordings were acquired from each waveform and averaged. The mice were euthanized by carbon dioxide inhalation at the end of the recordings.
3
Electrical Stimulation of Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The electric stimulation in cultures was performed with STG4000 (Multichannel Systems, Reutlingen, Germany) pulse generator using: a biphasic ramp waveform (100 µAmp, 20 Hz, 1 h); biphasic rectangular waveform (100 µAmp, pulse duration 25 ms, 20 Hz, 1 h) and biphasic sine (100 µAmp, 20 Hz, 1 h). The electric current was delivered to cultures using a c-dish carbon electrode plate (Ion Optix, Westwood, MA, Unites States). Between uses, the c-plate was incubated in 70% ethanol (15 min), followed by a washed with distilled water (15 min), and dried (1 h).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!