Acinetobacter calcoaceticus and Stenotrophomonas maltophilia, previously isolated from DW,31 (link) were grown overnight at 25 °C and under agitation (120 rpm) in R2A broth medium according to Gomes et al.32 (link) Afterwards, bacterial cells were harvested by centrifugation (Eppendorf centrifuge 5810R) at 3777 × g, 15 min, and resuspended in synthetic tap water (STW) composed by 100 mg L−1 NaHCO3 (Fisher Scientific, Leicestershire, UK), 13 mg L−1 MgSO4·7H2O (Merck, Darmstadt, Germany), 0.7 mg L−1 K2HPO4 (Applichem Panreac, Darmstadt, Germany), 0.3 mg L−1 KH2PO4 (CHEM-LAB, Zedelgem, Belgium), 0.01 mg L−1 (NH4)2SO4 (Labkem, Barcelona, Spain), 0.01 mg L−1 NaCl (Merck, Darmstadt, Germany), 0.001 mg L−1 FeSO4·7H2O (VWR PROLABO, Leuven, Belgium), 1 mg L−1 NaNO3 (Labkem, Barcelona, Spain), 27 mg L−1 CaSO4 (Labkem, Barcelona, Spain), 1 mg L−1 humic acids (Sigma-Aldrich, Steinheim, Germany).33,34 The cell density was adjusted to 1 × 106 CFU per mL for further experiments.
L 1 nacl
Manufactured by Merck Group
Sourced in Germany
The L-1 NaCl is a laboratory equipment used for the measurement and analysis of sodium chloride (NaCl) concentrations. It is a compact and reliable device designed to provide accurate and precise results. The core function of the L-1 NaCl is to measure the concentration of NaCl in various liquid samples, such as water, food, or pharmaceutical products.
Automatically generated - may contain errors
Lab products found in correlation
L 1 mgso4 7h2o, by Merck Group (2 mentions)
Centrifuge 5810r, by Eppendorf (1 mentions)
Cacl2 2h2o, by Merck Group (1 mentions)
Cellulose nitrate filter, by Sartorius (1 mentions)
L 1 yeast extract, by Merck Group (1 mentions)
Chromeleon 7, by Thermo Fisher Scientific (1 mentions)
Gswp04700, by Merck Group (1 mentions)
G3000swxl hplc column, by Tosoh (1 mentions)
Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions)
Tskgel swxl guard column, by Tosoh (1 mentions)
Nutrient broth, by Merck Group (1 mentions)
L 1 peptone, by Merck Group (1 mentions)
5 protocols using l 1 nacl
1
Growth and Preparation of Acinetobacter and Stenotrophomonas
Check if the same lab product or an alternative is used in the 5 most similar protocols
Acinetobacter calcoaceticus and Stenotrophomonas maltophilia, previously isolated from DW,31 (link) were grown overnight at 25 °C and under agitation (120 rpm) in R2A broth medium according to Gomes et al.32 (link) Afterwards, bacterial cells were harvested by centrifugation (Eppendorf centrifuge 5810R) at 3777 × g, 15 min, and resuspended in synthetic tap water (STW) composed by 100 mg L−1 NaHCO3 (Fisher Scientific, Leicestershire, UK), 13 mg L−1 MgSO4·7H2O (Merck, Darmstadt, Germany), 0.7 mg L−1 K2HPO4 (Applichem Panreac, Darmstadt, Germany), 0.3 mg L−1 KH2PO4 (CHEM-LAB, Zedelgem, Belgium), 0.01 mg L−1 (NH4)2SO4 (Labkem, Barcelona, Spain), 0.01 mg L−1 NaCl (Merck, Darmstadt, Germany), 0.001 mg L−1 FeSO4·7H2O (VWR PROLABO, Leuven, Belgium), 1 mg L−1 NaNO3 (Labkem, Barcelona, Spain), 27 mg L−1 CaSO4 (Labkem, Barcelona, Spain), 1 mg L−1 humic acids (Sigma-Aldrich, Steinheim, Germany).33,34 The cell density was adjusted to 1 × 106 CFU per mL for further experiments.
+ Expand
Acinetobacter calcoaceticus and Stenotrophomonas maltophilia, previously isolated from DW,31 (link) were grown overnight at 25 °C and under agitation (120 rpm) in R2A broth medium according to Gomes et al.32 (link) Afterwards, bacterial cells were harvested by centrifugation (Eppendorf centrifuge 5810R) at 3777 × g, 15 min, and resuspended in synthetic tap water (STW) composed by 100 mg L−1 NaHCO3 (Fisher Scientific, Leicestershire, UK), 13 mg L−1 MgSO4·7H2O (Merck, Darmstadt, Germany), 0.7 mg L−1 K2HPO4 (Applichem Panreac, Darmstadt, Germany), 0.3 mg L−1 KH2PO4 (CHEM-LAB, Zedelgem, Belgium), 0.01 mg L−1 (NH4)2SO4 (Labkem, Barcelona, Spain), 0.01 mg L−1 NaCl (Merck, Darmstadt, Germany), 0.001 mg L−1 FeSO4·7H2O (VWR PROLABO, Leuven, Belgium), 1 mg L−1 NaNO3 (Labkem, Barcelona, Spain), 27 mg L−1 CaSO4 (Labkem, Barcelona, Spain), 1 mg L−1 humic acids (Sigma-Aldrich, Steinheim, Germany).33,34 The cell density was adjusted to 1 × 106 CFU per mL for further experiments.
2
Microbial Community Cultivation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the cells of a microbial community originating from an activated sludge basin of a wastewater treatment plant (Eilenburg, Saxonia, Germany—51°27’39.4″ N, 12°36’17.5″ E) as our model microbial community described in Liu et al. [21 (link)]. The entire workflow employed in this study, including the experimental scheme of the sequential batch cultivation and treatment of the microbial community, is presented in Supplementary File S1: Figure S1A,B . The activated sludge sample (30 mL thawed inoculum) was first cultured in a 1-L batch flask in 300 mL medium at 30 °C and 125 rpm for 17 h to reduce the undegraded and inorganic particles from the sludge. The second and third batches (each also in 1-L flasks) were inoculated with an initial optical density (OD) of 0.05 (OD600nm, d = 0.5 cm), each, and cultivated in the same way.
The medium was a mixture of 98% synthetic wastewater and 2% peptone. The medium constituted 0.198 g L−1 peptone (from meat), 0.2 g L−1 meat extract, 0.219 g L−1 yeast extract, 0.1 g L−1 glucose, 0.49 g L−1 Na-propionate (filtered), 0.0059 g L−1 CaCl2·2H2O, 0.0294 g L−1 KCl, 0.06 g L−1 NaCl, 0.04 g L−1 K2HPO4, 0.2156 g L−1 KH2PO4 and 0.0196 g L−1 MgSO4·7H2O, purchased from: Merck KGaA (Darmstadt, Germany), SERVA Electrophoresis GmbH (Heidelberg, Germany) and Carl Roth GmbH (Karlsruhe, Germany).
The medium was a mixture of 98% synthetic wastewater and 2% peptone. The medium constituted 0.198 g L−1 peptone (from meat), 0.2 g L−1 meat extract, 0.219 g L−1 yeast extract, 0.1 g L−1 glucose, 0.49 g L−1 Na-propionate (filtered), 0.0059 g L−1 CaCl2·2H2O, 0.0294 g L−1 KCl, 0.06 g L−1 NaCl, 0.04 g L−1 K2HPO4, 0.2156 g L−1 KH2PO4 and 0.0196 g L−1 MgSO4·7H2O, purchased from: Merck KGaA (Darmstadt, Germany), SERVA Electrophoresis GmbH (Heidelberg, Germany) and Carl Roth GmbH (Karlsruhe, Germany).
3
Air Sampling of Microbial Diversity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Air samples were collected by impaction with a single-stage air sampler Microflow α (AQUARIA srl, Italy), holding 380 jets with a diameter of 1 mm. The sampler was placed in a central position of the cave at 1 m height. The impaction flow rate was set at 120 L min−1, and the airstream was directed on 90-mm-diameter agar Petri dishes containing different growth media, mainly Lysogeny Broth Agar (LBA) (10 g L−1 NaCl (Sigma-Aldrich, USA), 5 g L−1 yeast extract (Merck, Germany), 15 g L−1 agar (Difco, Michigan) and Plate Count Agar (PCA) (Difco, Michigan), or on cellulose nitrate filters with a pore size of 0.45 µm (Sartorius, Germany). Different sampling volumes, between 250 and 1000 L, were collected in duplicate. Filters were stored at −20 °C in sterile tubes until genomic DNA extraction.
4
Antibody Characterization by Size Exclusion Chromatography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Size exclusion chromatography was used to assess antibody yield, purity, and the amount of HMWI. High‐performance liquid chromatography was carried out by isocratic elution on a Dionex UltiMate 3000 HPLC system equipped with a diode array detector (Thermo Scientific, Waltham, MA, USA). The running buffer was 50 mmol L‐1 sodium phosphate buffer with 150 mmol L‐1 NaCl (Sigma‐Aldrich) at a pH of 7.0, prepared with 0.22 µm filtration (GSWP04700, Merck KGaA). 10 µL of a 0.2 µm vacuum‐filtered sample (0.2 µm GHP AcroPrep™ 96 filter plate; Pall Life Sciences, Ann Arbor, MI, USA) was applied to a TSKgel® G3000SWXL HPLC Column (5 µm, 7.8 × 300 mm) with a TSKgel SWXL Guard Column (7 µm, 6.0 × 40 mm; Tosoh, Tokyo, Japan). Chromeleon™ 7 software (Thermo Scientific) was used to monitor the signals at 215 nm (for HMWI) and 280 nm (for purity and yield). Product purity was defined by the ratio of product peak area (monomer) to sum of all peak areas. Product yield was calculated dividing the product peak area (monomer) of the flocculated material by the product peak area (monomer) of the cell culture broth.
5
Pseudomonas taetrolens LMG 2336 Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pseudomonas taetrolens LMG 2336 (from the Belgian Coordinated Collection of Microorganisms, Ghent, Belgium) was used. The microorganism was inoculated on Nutrient Broth (NB, containing 1 g L−1 meat extract, 2 g L−1 yeast extract, 5 g L−1 peptone and 5 g L−1 NaCl, all from Sigma-Aldrich, Steinheim, Germany) agar (20 g L−1, VWR Chemicals, West Chester, PA, USA) plates and incubated at 30 °C for 48 h. A loopful from a fresh NB agar plate was inoculated in a 500 mL Erlenmeyer flask containing 100 mL of NB broth (Sigma-Aldrich) (ratio medium air 1:4). The inoculum was incubated in an orbital shaker (Model G25; New Brunswick Scientific Co., Edison, NJ, USA) at 250 rpm and 30 °C for 10 h. After this time, biomass was separated by centrifugation (10,000 rpm, 10 min) and was subsequently used as a bulk starter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!