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Human intesticult organoid growth media

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Human IntestiCult organoid growth media is a cell culture media formulation designed to support the growth and maintenance of human intestinal organoids in vitro.

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Lab products found in correlation

Rock inhibitor, by STEMCELL (2 mentions) Primocin, by InvivoGen (2 mentions) A83 01, by Bio-Techne (2 mentions) Gastrin 1, by Merck Group (2 mentions) Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (2 mentions) Tryple express, by Thermo Fisher Scientific (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions) Matrigel, by Corning (2 mentions)

Close spelling variants of this product

Intesticult (16 citations) IntestiCult Organoid Growth Media (11 citations) IntestiCult media (4 citations) Human Intesticult (3 citations)

2 protocols using human intesticult organoid growth media

1

Culturing Human Intestinal Organoids

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Polyps were dissociated and washed as described in the COLON MAP scRNA-seq, Encapsulation and Library Generation section. After dissociation, cells were washed 3 times with PBS containing 10 μM ROCK inhibitor (STEMCELL Technologies) and pelleted by quick-pulse centrifugation for 7 s. Human organoid models were generated from COLON MAP individuals of both sexes (70% female, 30% male). Polyp-derived cells were grown with Human IntestiCult organoid growth media (STEMCELL Technologies) supplemented with 10 μM Y-27632, 10 nM Gastrin I (Sigma-Aldrich), 1 mM N-acetyl-L-cysteine (Sigma-Aldrich), 500 nM A83–01 (Tocris), 50 ng/mL FGF-2 (Thermo Fisher), 100 ng/mL IGF-1 (BioLegend), 100 μg/mL Primocin (InvivoGen), and Matrigel (Corning) in a 3:1 ratio of Matrigel to media. Media was replaced every 2–3 days, and passaging was performed by dissociating the organoids in TrypLE Express (Thermo Fisher) with 10 μM Y-27632 for 15 minutes at 37°C while shaking and triturating.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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2

Culturing Human Intestinal Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyps were dissociated and washed as described in the COLON MAP scRNA-seq, Encapsulation and Library Generation section. After dissociation, cells were washed 3 times with PBS containing 10 μM ROCK inhibitor (STEMCELL Technologies) and pelleted by quick-pulse centrifugation for 7 s. Human organoid models were generated from COLON MAP individuals of both sexes (70% female, 30% male). Polyp-derived cells were grown with Human IntestiCult organoid growth media (STEMCELL Technologies) supplemented with 10 μM Y-27632, 10 nM Gastrin I (Sigma-Aldrich), 1 mM N-acetyl-L-cysteine (Sigma-Aldrich), 500 nM A83–01 (Tocris), 50 ng/mL FGF-2 (Thermo Fisher), 100 ng/mL IGF-1 (BioLegend), 100 μg/mL Primocin (InvivoGen), and Matrigel (Corning) in a 3:1 ratio of Matrigel to media. Media was replaced every 2–3 days, and passaging was performed by dissociating the organoids in TrypLE Express (Thermo Fisher) with 10 μM Y-27632 for 15 minutes at 37°C while shaking and triturating.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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