Activated charcoal, agar agar Kobe I, and mannitol were purchased from Carl Roth (Karlsruhe, Germany). Soy flour (Hensel Bio-Voll-Soja) was from W. Schoenenberger GmBH Co. KG (Magstadt, Germany). HPLC grade methanol was purchased from VWR International GmbH (Bruchsal, Germany). Ethyl acetate was distilled prior to use. For preparation of media, double distilled water was used. All other chemicals were from Sigma-Aldrich (Taufkirchen, Germany). Petri dishes were purchased from Greiner Bio-One GmbH (Frickenhausen, Germany).
Activated charcoal
Manufactured by Carl Roth
Sourced in Germany
Activated charcoal is a highly porous form of carbon that has been treated to increase its surface area and adsorption capacity. It is a versatile laboratory material used for a variety of applications, including the removal of impurities, the purification of liquids and gases, and the adsorption of organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade methanol, by Avantor (1 mentions)
Agar agar kobe 1, by Carl Roth (1 mentions)
Mannitol, by Carl Roth (1 mentions)
Petri dishes, by Greiner (1 mentions)
Noradrenaline, by Merck Group (1 mentions)
Sephadex g 15, by GE Healthcare (1 mentions)
Adenosine triphosphate (atp), by Roche (1 mentions)
Adenosine diphosphate (adp), by Roche (1 mentions)
Ethyl acetate, by Carl Roth (1 mentions)
Methanol, by Carl Roth (1 mentions)
Toluene, by Carl Roth (1 mentions)
Hypoxia chamber, by Biospherix (1 mentions)
Oxycycler controller, by Biospherix (1 mentions)
3 protocols using activated charcoal
1
Microbial Growth Media Preparation
2
Synthesis and Analysis of Inositol Phosphates
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Prostaglandin E1 was obtained from Cayman Chem. Comp. (Ann Arbor, MI, USA); BCA-protein-assay-reagent from Pierce (Rockfield, IL, USA); pentobarbital-sodium (Nembutal R) from Wirtschaftsgenossenschaft dtsch. Tierärzte (Hannover, Germany); ATP, GTP, UTP, CTP, ADP, and GDP were obtained from Roche Diagnostics (Mannheim, Germany); inositol(1,4)P2, inositol(2,4)P2, inositol(4,5)P2, inositol(5,6)P2, inositol(1,4,5)P3, noradrenaline, adrenaline, and silicic acid SIL-350 were purchased from Millipore-Sigma (Darmstadt, Germany); Sephadex G15, HL-Q-Sepharose HP, [5,6(n)-3H] PGE1, [8-3H] GTP, [8-14C] ADP, [2-3H] inositol with stabilizer PT6-271, [2-3H] inositol(1,4)P2, and [3H] inositol(1,4,5)P3 were obtained from GE-Healthcare (Solingen, Germany); activated charcoal, toluene, ethyl acetate, methanol, Rotiscint 11, and Rotiscint 22 were obtained from Carl Roth (Karlsruhe, Germany); all other chemicals of reagent grade were from E. Merck (Darmstadt, Germany).
A regiospecific ionsitol-P5/inositol-P4-phosphohydrolase from Dictyostelium discoideum was exploited for the synthesis of inositol (1,6)-bisphosphate [35 (link)].
Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 were drawn with Microsoft office Excel 2007 (12.0.6787.5000) SP3 MSO (12.0.6785.5000).
A regiospecific ionsitol-P5/inositol-P4-phosphohydrolase from Dictyostelium discoideum was exploited for the synthesis of inositol (1,6)-bisphosphate [35 (link)].
3
Hypoxia-Induced Cardiac Remodeling in Mice
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Mice were housed under normoxic (21% oxygen in room air) or hypoxic conditions in a hypoxia chamber (BioSpherix, Parish, NY, USA) to mimic lower partial pressure of inspired oxygen present at high altitude. To avoid hypobaropathy, FiO2 was decreased in the hypoxia chamber by 1%/day from 21% to 12% and was followed by a continuous exposure of 12% oxygen, which mimics an altitude of ≈4200 m above sea level (Figure 1A ). Following acclimatization for the duration of 1 week, mice housed under hypoxic conditions were subjected to LVPO by TAC (HxTAC group) or to sham surgery (HxSham group). Mice continuously housed under normoxic conditions post sham surgery (NxSham group) or TAC (NxTAC group) served as controls (Figure 1A ). FiO2 in the hypoxia chamber was automatically adjusted by ventilation and controlled by an OxyCycler controller (BioSpherix). Soda lime (Dräger Medizintechnik, Lübeck, Germany) and activated charcoal (Roth, Karlsruhe, Germany) were used for the removal of CO2 and ammonia. CO2 concentration was monitored to not exceed 10 000 ppm (Fuehler Systeme, Nürnberg, Germany).
23 (link) Mice housed under hypoxic conditions received food and water ad libitum. Because hypoxia exposure decreases food intake in mice, mice housed under normoxic conditions were given equivalent amounts of food with unrestricted access to water (pair‐feeding).
4 (link)
23 (link) Mice housed under hypoxic conditions received food and water ad libitum. Because hypoxia exposure decreases food intake in mice, mice housed under normoxic conditions were given equivalent amounts of food with unrestricted access to water (pair‐feeding).
4 (link)
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