The pomegranate variety Ganesh was procured in Palampur (H.P.), India (32.1109° N, 76.5363° E). All reference standards used were obtained from Sigma-Aldrich. The total dietary fiber kit (K-TDFR, 200A) was procured from Megazyme (Wicklow, Ireland).
K tdfr 200a
Manufactured by Megazyme
Sourced in Ireland
K-TDFR-200A is a laboratory equipment designed for the determination of total dietary fiber in food samples. The equipment utilizes enzymatic and gravimetric methods to measure the total dietary fiber content in a variety of food matrices.
Automatically generated - may contain errors
Lab products found in correlation
K tsta 100a, by Megazyme (1 mentions)
K amyl, by Megazyme (1 mentions)
Folin reagent, by Merck Group (1 mentions)
Fluorescein fl disodium salt, by Merck Group (1 mentions)
2 20 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
K rstar, by Megazyme (1 mentions)
6 protocols using k tdfr 200a
1
Pomegranate Variety Ganesh Fiber Analysis
2
Starch, Amylose, and Dietary Fiber Analysis
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Total starch (K-TSTA-100A), amylose/amylopectin assay (K-AMYL), and dietary fibre (K-TDFR-200A) kits were purchased from Megazyme Ltd. (Bray, Ireland), and the analyses were performed according to the manufacturer’s protocols.
3
Dietary Fiber Composition Analysis
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The soluble DF (SDF) and insoluble DF (IDF) contents were determined according to AACC Method 32-07 using the Megazyme total dietary fibre assay kit K-TDFR-200A. The total DF (TDF) was calculated as a sum of SDF and IDF. The major DF components were determined as total arabinoxylan and water-soluble arabinoxylan (WE) according to the colorimetric method of Hashimoto et al. (1987 ). The total β-glucan, water-insoluble β-glucan (WUE) and fructan were estimated by the AACC Approved Methods 32-23 and 32-32, respectively. The water-insoluble arabinoxylan (WUE) was calculated as a difference between total arabinoxylan and WE arabinoxylan. The water-soluble β-glucan (WE) was the difference between total β-glucan content and its WUE fraction. The β-glucan and fructan contents were analysed using the Megazyme β-glucan mixed linkage and fructan assay kits K-BGLU and K-FRUC, respectively. All analyses were run in triplicates.
4
Dietary Fiber Content Determination
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The determination of the total dietary fiber content as well as the contents of soluble and insoluble dietary fiber was carried out according to the AOAC 991.43 (Association of Analytical Chemists [AOAC], 2000 ) method using an enzyme assay kit (K‐TDFR‐200A, Megazyme Ltd).
5
Comprehensive Bread Composition Analysis
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The moisture content was determined by the weight loss after drying in a 120 °C oven for 24 hours. The total carbohydrate content was measured as the sum of the fibre and starch content. The Megazyme K-TSTA kit (Megazyme, Co. Wicklow, Ireland) was used to measure the total starch content of samples containing resistant starch following the manufacturer’s instructions. This method variant uses dimethyl sulfoxide and boiling bath, and dissolution in dimethyl sulfoxide at 100 °C is effective to solubilize all starches in the bread, including the resistant starch. The fibre content was determined using the Megazyme total fibre assay kit (K-TDFR-200A, Megazyme, Co. Wicklow, Ireland) following the manufacturer’s instructions. The K-TDFR-200A kit measure mainly the cell wall polysaccharides and some RS.
6
Dietary Fiber and Antioxidant Analysis
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All chemicals used were of reagent grade. Megazyme (Dublin, Ireland) reagents were used for total dietary fiber (K-TDFR-200A), total starch (K-RSTAR), and free glucose (K-GLUC) content. Folin reagent, 2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylch-roman-2-acid (Trolox), fluorescein (FL) disodium salt, 2,20-azobis (2-methylpropionamidine) dihydrochloride (AAPH), gallic acid, and caramel were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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