All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl2 and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).
Culture mediums
Culture mediums are laboratory products used to support the growth and propagation of microorganisms, cells, or tissues in a controlled environment. They provide the necessary nutrients, pH, and other conditions required for the targeted organisms to thrive. Culture mediums are essential for various applications in microbiology, cell biology, and biotechnology.
Lab products found in correlation
3 protocols using culture mediums
Transglutaminase 2 Purification and Detection
All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl2 and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).
Transglutaminase 2 Purification and Detection
All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl2 and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).
Transglutaminase 2 Purification and Detection
All restriction enzymes, X-gal, and IPTG were obtained from Fermentas (Lithuania). dNTP and Taq DNA polymerase were purchased from Roche (Germany). Culture mediums were purchased from Gibco (USA). Cellfectin II and TRIZOL were purchased from Invitrogen (USA). Coomassie brilliant blue, HCL, NaOH and boric acid were from Merck (Germany). Nitrocellulose membrane from Schleicher & Schuell BioScience (Germany). Gentamicin, Tetracycline, Kanamycin, Tris, SDS, Protease inhibitor, Laemmli buffer, DAB, dansylcadaverine, N,N dimethyl casein, EDTA, Cacl2 and DTT were obtained from Sigma (USA). Primers were synthesized by Pishgam biotech (Iran). cDNA synthesis were performed by Takara kit (Japan). Spodoptera frugiperda (Sf9) cells were from National Cell Bank of Iran (NCBI, Pasteur Institute of Iran). Recombinant human transglutaminase 2/TGM2 was purchased from R&D systems (USA). Anti-TG2 antibody was from Abcam (UK) and anti-rabbit IgG was obtained from Santa Cruz Biotechnology (USA).
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