The HMC3 human microglial cell line (ATCC CRL-3304) and EMEM medium (ATCC 30–2003) were purchased from American Type Culture Collection (ATCC, Manassas, VA). BrdU (59–14-3) and mouse anti-BrdU (B2531) monoclonal antibody (mAb) were purchased from Sigma (St. Louise, MO). BAY 11–7082 was obtained from Cayman Chemical (Ann Arbor, MI, cat# 10010266). Human IL-6 DuoSet ELISA (DY206) kits were purchased from R&D Systems (Minneapolis, MN). Phospho-p53 (Ser 15) antibody (#9284), p53 mouse mAb (#2524), NF-κB p65 rabbit mAb (#8242), p21 Waf1/Cip1 rabbit mAb (#2947), and β-Actin mouse mAb (#3700) were purchased from Cell Signaling (Danvers, MA). Alexa Fluor-594 labeled goat anti-mouse IgG (#A10680) and Alexa Fluor-647 labeled goat anti-rabbit IgG (#A21244) were purchased from Thermo Fisher (Waltham, MA). Mouse anti-human p16 mAb (cat# 51–1325GR) was obtained from BD Biosciences. Mouse anti-human CD68 mAb (Cat# 14–0688-82, clone: KP1) was obtained from Thermo Fisher. Pro-Long Gold Anti-Fade Reagent (#9071) was obtained from Cell Signaling Technology (Danvers, MA).
P21 waf1 cip1 rabbit mab
Manufactured by Cell Signaling Technology
P21 Waf1/Cip1 rabbit mAb is an antibody product from Cell Signaling Technology. It is a monoclonal antibody that recognizes the p21 protein, which is involved in cell cycle regulation.
Automatically generated - may contain errors
Lab products found in correlation
Nf κb p65 rabbit mab, by Cell Signaling Technology (1 mentions)
β actin mouse mab, by Cell Signaling Technology (1 mentions)
Bay 11 7082, by Cayman Chemical (1 mentions)
Hmc3 human microglial cell line, by American Type Culture Collection (1 mentions)
Alexafluor 594 labeled goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 labeled goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade reagent, by Cell Signaling Technology (1 mentions)
Emem medium, by American Type Culture Collection (1 mentions)
Anti igm f ab 2, by Jackson ImmunoResearch (1 mentions)
Ki67 mouse mab, by Agilent Technologies (1 mentions)
Gapdh mouse mab, by Abcam (1 mentions)
2 protocols using p21 waf1 cip1 rabbit mab
1
Characterizing Human Microglial Cell Responses
2
Multiplex Western Blot Antibody Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cyclin D1/2 mouse monoclonal antibody (mAb) (1:1000; Sigma Aldrich #05-362), CDK4 mouse mouse mAb (1:2000; Cell Signaling Technology #2906), GAPDH mouse mAb (1:15.000; abcam #ab8245), Ki-67 mouse mAb (1:100; Dako, #M7240), MMP14 mouse mAb (1:1000; LSBio #LS-C8915), p21 Waf1/Cip1 rabbit mAb (1:1000; Cell Signaling Technology #2947), PCNA mouse mAb (1:2000; Cell Signaling Technology #2586), phospho-p53 (Ser15) mouse mAb (1:1000; Cell Signaling Technology #9286), phospho-vimentin (Ser56) rabbit polyclonal (1:1000; Cell Signaling Technology #3877), SYK mouse mAb (1:1000; abcam #ab3993), cleaved/uncleaved-PARP rabbit polyclonal (1:1000; Cell Signaling Technology #9542), anti-IgM F (ab)2 (Jackson Immuno Research Laboratories, #309-005-107).
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