Fcεr1

ILC2 isolation and sorting from lung tissue was performed as described previously^{27 (link)}. Briefly, lungs were perfused with 10 ml PBS through right ventricle of heart, and then filled with 1 ml RPMI medium with Liberase TM (50 μg/ml final concentration) and DNase I (1 μg/ml final concentration) and digested in 5 ml RPMI digestion medium for 30–45 min at 37 °C with vortexing every 10 min. The resultant samples were mashed by 70 μm cell strainers, washed with Dulbecco’s modified Eagle media [DMEM; supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Pittsburgh, PA)], and the remaining red blood cells were lysed. Cell suspensions were used for subsequent flow cytometry staining. For ILC2 sorting, total lung cells were stained with lineage cocktail Abs, against B220, CD3, CD4, CD5, CD8α, CD11b, CD11c, CD19, Gr-1, TCRβ, Ter-119, γδTCR, NK1.1, and FcεR1 (eBioscience, San Diego, CA); anti-CD45 Ab (eBioscience); anti-ST2 Ab (MD Biosciences, Oakdale, MN); and anti-CD90.2 Ab (eBioscience), at 4 °C for 30 min. ILC2 were defined as Lin⁻CD90.2⁺CD45⁺ST2⁺ and sorted by FACSAria (BD Biosciences). The average purity of ILC2 is >98%.