Anhydrous na2co3

Manufactured by Merck Group

Sourced in Germany

Anhydrous sodium carbonate (Na2CO3) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and has a mildly alkaline pH. Anhydrous Na2CO3 is primarily used as a desiccant, pH buffer, and as a source of carbonate ions in various chemical reactions and analyses.

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7 protocols using anhydrous na2co3

Purification of Recombinant Proteins

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Escherichia coli Trans 5α and BL21(DE3) strains and affinity chromatography ProBond resin (Ni²⁺) were bought from TransGen Biotech (Beijing, China) and Promega (Madison, WI), respectively. Trypsase, BamHI and HindIII were merchandised from New England Biolabs (Beijing, China). Tris base of molecular biology grade, NaCl, CaCl₂, anhydrous Na₂CO₃, NH₄HCO₃, NaHCO₃, and NaOH were purchased from Sigma-Aldrich Co. (St Louis, USA). HCl solution (37%), acetonitrile, formic acid (FA), glacial acetic acid were obtained from Thermo Fisher Scientific (Billerica, USA).
Ten percent sodium dodecyl sulfate (SDS) solution, ammonium persulfate, tricine, 5× sample buffer, glycine, 30% acrylamide/bis (29:1), and N,N,N',N' -tetramethylethylenediamine (TEMED) are electrophoresis purity reagents purchased from Bio-Rad Laboratories Inc. (Hercules, USA). Exact-Pro broad range (11-180kDa) pre-stained protein ladder was from Genstar (Beijing, China). Na₂S₂O₃, Coomassie brilliant blue were purchased from Sigma-Aldrich Co. (St Louis, USA). Iso-propyl-thio-β-galactoside (IPTG), kanamycin and imidazole were purchased from Sigma (St Louis, USA). Bacto-yeast extract and Bacto-tryptone were obtained from OXOID (UK).

Liu J., Feng X., Jin Y., Sun Z., Meng H., Zhang Z., Hu L, & Yang Z. (2017). The antitumor activity and preliminary modeling on the potential mechanism of action of human peroxiredoxin-5. Oncotarget, 8(16), 27189-27198.

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Phytochemical Characterization of Helleborus purpurascens

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All reagents used in this study were of analytical grade, purchased from commercial sources and used without further purification. Ethanol, methanol, chloroform, dichloromethane, FeCl₃·6H₂O, FeSO₄·7H₂O and anhydrous Na₂CO₃ were from Sigma Aldrich (München, Germany). 1,10-Phenantroline; Folin–Ciocalteu phenol reagent (2 N); 2,2-diphenyl-1-picrylhydrazyl reagent; gallic acid; ascorbic acid; AgNO₃; sodium citrate; sodium carbonate; potassium persulfate; sodium phosphate; ammonium molybdate; and potassium chloride of 99% were also purchased from Sigma-Aldrich (München, Germany). Propyl gallate (purum) was purchased from Fluka (Buchs, Switzerland). Chitosan (MW 100,000–300,000) was purchased from Acros Organics (Verona, Italy). Ultrapure water (DDW) was used throughout the experiments.
The Helleborus purpurascens Waldst. & Kit. samples (whole plant: stem (28 cm in height), leaves, roots and rhizome (8 cm)) were harvested in March 2023 from an area in Prahova County, Romania (geographic coordinates: 45°20’46.4689750856423” N, 25°33’22.9191970825194” E), and taxonomically authenticated at the University of Medicine and Pharmacy Craiova, Romania.

Segneanu A.E., Vlase G., Vlase T., Sicoe C.A., Ciocalteu M.V., Herea D.D., Ghirlea O.F., Grozescu I, & Nanescu V. (2023). Wild-Grown Romanian Helleborus purpurascens Approach to Novel Chitosan Phyto-Nanocarriers—Metabolite Profile and Antioxidant Properties. Plants, 12(19), 3479.

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Validated Standards for Polyphenol Analysis

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Standards of gallic acid (99%), 3-hydroxytyrosol (99.5%), (−)- gallocatechin (≥98%), caftaric acid (≥97%), (−)-epigallocatechin (≥95%), (+)-catechin (≥99%), (−)-epicatechin (≥95%), (−)- epigallocatechin gallate (≥95%), caffeic acid (99%), syringic acid (≥95%), coumaric acid (99%), ferulic acid (≥99%), piceatannol (99%), trans-resveratrol (≥99%), quercetin hydrate (95%), cinnamic acid (99%), quercetin 3-b-D-glucoside (≥90%), kaempferol-3-glucoside (≥99%) and malvidin-3-O- glucoside chloride (≥95%) were purchased from Sigma–Aldrich. The standard of 2-(4-hydroxyphenyl) ethanol (tyrosol) (≥99.5%) was obtained from Fluka (Buchs, Switzerland). Stock solutions of the above mentioned compounds were prepared in methanol at concentration levels of 1000 µgm l⁻¹. Calibration standards were dissolved in the initial mobile phase of each method (LMWP or anthocyanins, respectively). HPLC-grade Acetonitrile (MeCN) and formic acid (FA) were acquired from Mallinckrodt Baker (Inc. Phillipsburg, NJ, USA). Primary-secondary amine (PSA) and octa- decylsilane (C₁₈) were both obtained from Waters (Milford, MA, USA). Reagent grade NaCl, anhydrous Na₂CO₃, anhydrous MgSO₄ and anhydrous CaCl₂ were purchased from Sigma–Aldrich. Ultrapure water was obtained from a Milli-Q system (Millipore, Billerica, MA, USA).

Alonso R., Berli F.J., Fontana A., Piccoli P, & Bottini R. (2021). Abscisic Acid’s Role in the Modulation of Compounds that Contribute to Wine Quality. Plants, 10(5), 938.

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Phytochemical Screening of Extracts

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Methanol, HCl, H₂SO₄, lead acetate, sodium hydroxide and ethanol were acquired from Wako Pure Chemicals Ind., Ltd, Japan. NaCl, LB media, and bacto agar were obtained from Liofilchem in Italy. Fehling's solution A and B, FeCl₃, CuSO₄, sodium nitroprusside, DMSO, CHCl₃ were obtained from Merck in Germany. Ampicillin discs were obtained from Bio-Rad in USA. Anhydrous Na₂CO₃, ninhydrin and sodium citrate were acquired from Sigma-Aldrich in Germany.

Rahman M.M., Islam M.R., Talukder M.E., Atif M.F., Alam R., Uddin A.F., Anis-Ul-Haque K.M., Islam M.S., Uddn M.J, & Akhter S. (2024). Unveiling therapeutic efficacy of extract and multi-targeting phytocompounds from Christella dentata (Forssk.) Brownsey & Jermy against multidrug-resistant Pseudomonas aeruginosa. RSC Advances, 14(9), 6096-6111.

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Folin-Ciocalteu Assay and ZIF-8 Preparation

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The preparation of solutions along this work was done with Milli-Q water (MQW, resistivity > 18 MΩ cm, Millipore, Bedford, MA, USA) and all the chemicals were analytical grade, unless otherwise stated.
The Folin-Ciocalteu (FC) assays were performed using FC reagent and anhydrous sodium carbonate. The FC reagent was prepared by diluting a commercial FC solution (2 M with respect to the acid, Sigma-Aldrich) to a concentration of 0.5 M with MQW.
The alkali solution of 15% (m/v) sodium carbonate was weekly prepared by weighing anhydrous Na₂CO₃ (≥ 98%, Sigma-Aldrich) to a 20 mL MQW.
The methanolic (HPLC grade) ZIF-8 dispersion (10 g L⁻¹) was prepared by weighing 20 mg of previously sieved MOF (50–100 µm) and then adjusting the volume to 2 mL with pure methanol (MeOH).
A 1 g L⁻¹ stock solution of gallic acid (GA, ≥ 99%, Sigma-Aldrich) was prepared by dissolving 50 mg of the solid in 50 mL MQW and stored at 4 °C in the darkness until used. Daily dilutions were carried out with MQW to prepare working standards in the range of 1–50 mg L⁻¹.
The same procedure was followed to perform the oenotannin assays (Martin Vialatte, France) in the analysis of fruit samples.

Martínez-Pérez-Cejuela H., Mesquita R.B., Simó-Alfonso E.F., Herrero-Martínez J.M, & Rangel A.O. (2023). Combining microfluidic paper-based platform and metal–organic frameworks in a single device for phenolic content assessment in fruits. Mikrochimica Acta, 190(4), 126.

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Phytochemical Characterization of Helleborus purpurascens

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Synthesis and Characterization of Ba2NaMoO5.5

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Ba₂NaMoO_5.5 was
synthesized by reaction between barium molybdate (BaMoO₄) and stoichiometric amounts of barium carbonate (BaCO₃) (Fluka, >99%) and anhydrous sodium carbonate Na₂CO₃ (Sigma–Aldrich, >99.5%). The stoichiometric
mixture was heated under oxygen flow at 973 K for 60 h with intermediate
regrinding steps. Samples were analyzed by X-ray and neutron diffraction
at room temperature.
The starting reagent BaMoO₄ was
synthesized by grinding barium carbonate BaCO₃ (Fluka,
>99%) and molybdenum trioxide MoO₃ (Alfa Aesar, >99.5%)
together in stoichiometric ratio and heating under dry oxygen at 1073
K for 45 h with intermediate regrinding steps. Samples were analyzed
by X-ray and neutron diffraction at room temperature (see Figure S1 in the Supporting Information).
Na₂Mo₂O₇ was synthesized for solution
calorimetry measurement purposes by heating a stoichiometric mixture
of anhydrous Na₂CO₃ (Sigma–Aldrich, 99.5%)
and Na₂MoO₄ (Sigma–Aldrich, 99.5%) under
dry oxygen at 773 K for 60 h with intermediary regrinding. The sample
then was checked by XRD and DSC measurements (see Figures S2 and S3 in the Supporting Information).
In
every analysis, no secondary phase was observed. Therefore,
the purity of every compound is expected to be better than 99.5%.

Kauric G., Epifano E., Martin P.M., van Eijck L., Bouëxière D., Clavier N., Guéneau C, & Smith A.L. (2020). Structural and Thermodynamic Investigation of the Perovskite Ba2NaMoO5.5. Inorganic Chemistry, 59(9), 6120-6130.

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